Mesenchymal Cell News Volume 7.35 | Sep 8 2015

Mesenchymal Stromal Cells Differentiating to Adipocytes Accumulate Autophagic Vesicles Instead of Functional Lipid Droplets
The authors investigated the structural and functional features of bone marrow mesenchymal stromal cells (BMSCs)-derived adipocytes as compared with adipocytes from adipose tissue, and the structure and functionality of lipid vesicles formed during BMSCs differentiation to adipocytes. [J Cell Physiol] Abstract

Femtosecond Laser Nano/Micro Patterning of Titanium Influences Mesenchymal Stem Cell Adhesion and Commitment
Three biomimetic textures were fabricated on titanium surface: micropits with nano-ripples in the pits, micropits with nano-ripples around the pits, and a texture with only nano-ripples. Mesenchymal stem cells grown on these surfaces displayed altered morphometric parameters, and modified their focal adhesions in term of number, size, and distribution depending on surface type. [Biomed Mater] Abstract

Migrational Changes of Mesenchymal Stem Cells in Response to Cytokines, Growth Factors, Hypoxia, and Aging
Using the in vitro scratch assay, scientists showed that the inflammatory cytokines, chemokines, and growth factors TNF-α, SDF-1, PDGF, and bFGF enhance migration of rat mesenchymal stem cells (MSCs) under normoxic conditions, while TNF-α, IFN-γ, PDGF, and bFGF promote MSC migration under hypoxic conditions. [Exp Cell Res] Abstract

Activation of PKA/CREB Signaling Is Involved in BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells
Researchers explored the detailed role of PKA/CREB signaling in BMP9-induced osteogenic differentiation. Using PKA inhibitors and a dominant negative protein of CREB, they investigated the effect of PKA/CREB signaling on BMP9-induced osteogenic differentiation. [Cell Physiol Biochem] Full Article

BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype
Mesenchymal stem cells (MSCs) were cultured with various gradients of bone morphogenetic protein 3 (BMP3) and BMP3/transforming growth factor (TGF)-β, and compared with cultures in basal and TGF-β media. Cell proliferation, glycosaminoglycan content, gene expression, and signaling proteins were measured to assess the effects of BMP3 and BMP3/TGF-β on MSCs. [Int J Mol Sci] Abstract | Download Full Article

Mesenchymal Stromal Cells Derived from Cervical Cancer Tumors Induce TGF-β1 Expression and IL-10 Expression and Secretion in the Cervical Cancer Cells, Resulting in Protection from Cytotoxic T Cell Activity
Researchers provide evidence that mesenchymal stromal cells (MSCs) derived from cervical tumors (CeCa) cocultured with CeCa cells induced significant expression of TGF-β1 and secretion of IL-10 by CeCa cells compared to MSCs derived from the normal cervix and normal bone marrow. [Cytokine] Abstract

Bone-Marrow Derived Mesenchymal Stem Cells Assembled with Low Dose BMP-2 in a Three Dimensional Hybrid Construct Enhances Posterolateral Spinal Fusion in Syngeneic Rats
Scientists hypothesized that the synergistic osteoinductive capacity of low dose bone bone morphogenetic protein 2 (BMP-2) combined with undifferentiated bone marrow derived stromal cells (BMSCs) is comparable to that of osteogenically differentiated BMSCs when used in a rodent model of posterolateral spinal fusion. [Spine J] Abstract

The Characteristics of Human Cranial Bone Marrow Mesenchymal Stem Cells
Bone marrow-derived mesenchymal stem cells from cranial bone waste and cells obtained from the iliac crest showed expression of cell surface markers associated with mesenchymal stem cells and multipotency traits such as differentiation into osteogenic and adipogenic lineages. [Neurosci Lett] Abstract

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BioLife Solutions Customer TiGenix Phase III Clinical Trial Meets Primary Endpoint
BioLife Solutions, Inc. announced that its customer TiGenix NV, a biopharmaceutical company focused on developing and commercializing novel therapeutics from its proprietary platforms of allogeneic expanded stem cells, recently disclosed that its lead compound Cx601 met the primary endpoint in the Phase III ADMIRE-CD trial of complex perianal fistula in Crohn’s Disease patients. [BioLife Solutions, Inc.] Press Release

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NEW Faculty Positions – Stem Cells and Regenerative Medicine (King Abdullah University of Science and Technology)

Postdoctoral Fellow – Cardiovascular Stem Cell Biology and Tissue Engineering (Yale University)

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Professorship – Cellular and/or Molecular Biology and Immunology (Nanjing University)

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Postdoctoral Position – Stem Cells (Technion – Israel Institute of Technology)

Postdoctoral Fellow/Research Associate – Leukemia, Stem Cells and Development (Josep Carreras Leukemia Research Institute)

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