TGF-β-Induced KDM4B Promotes Chondrogenic Differentiation of Human MSCs Investigators found that TGF-β dramatically induced the expression of KDM4B in mesenchymal stem cells (MSCs). When KDM4B was overexpressed, chondrogenic differentiation was significantly enhanced while KDM4B depletion by shRNA led to a significant reduction in chondrogenic potential. [Stem Cells] Abstract RECK (Reversion-Inducing Cysteine-Rich Protein with Kazal Motifs) Regulates Migration, Differentiation and Wnt/β-Catenin Signaling in Human Mesenchymal Stem Cells Knockdown of RECK in human mesenchymal stem cells (hMSCs) promoted monolayer regeneration and chemotactic migration of hMSCs, as demonstrated by scratch wound and chemotaxis assay analyses. Moreover, expression of endogenous RECK was upregulated upon osteogenic differentiation and diminished after adipogenic differentiation of hMSCs. [Cell Mol Life Sci] Abstract A Regulatory Loop Containing miR-26a, GSK3β and C/EBPα Regulates the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells Scientists analyzed the role of miR-26a in the regulation of human adipose-derived mesenchymal stem cell (hADSC) osteogenesis. The endogenous expression of miR-26a increased during the osteogenic differentiation. The overexpression of miR-26a promoted hADSC osteogenesis, whereas osteogenesis was repressed by miR-26a knockdown. [Sci Rep] Full Article Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application In vitro experiments showed that bone marrow-derived mesenchymal stem cells (BMSCs) cultured from C57BL/6J mice secreted estrogen (E2) in a time-dependent manner. In vivo studies identified a significantly increased E2 level in serum after a single administration of BMSCs, and a sustained elevation of E2 level upon a repetitive administration. [Sci Rep] Full Article Mesenchymal Stem Cells Suppress Neuronal Apoptosis and Decrease IL-10 Release via the TLR2/NFκB Pathway in Rats with Hypoxic-Ischemic Brain Damage Researchers studied the mechanisms by which mesenchymal stem cells mediate immunomodulation via the key effectors Toll-like receptor 2 (TLR2) and interleukin-10 (IL-10). [Mol Brain] Full Article Protease Inhibitors Enhance Extracellular Collagen Fibril Deposition in Human Mesenchymal Stem Cells The authors investigated the fate of collagen when it is in contact with mesenchymal stem cells and hypothesized that protease inhibition would enhance their extracellular deposition of collagen fibrils. [Stem Cell Res Ther] Full Article Macrophages Derived from THP-1 Promote the Osteogenic Differentiation of Mesenchymal Stem Cells through the IL-23/IL-23R/β-Catenin Pathway Researchers investigated the stimulatory effects of THP-1 macrophage conditioned medium on the osteogenic differentiation of human mesenchymal stem cells (MSCs) and the associated signaling pathways. The osteogenic differentiation of MSCs was induced after exposure to osteogenic differentiation medium. [Exp Cell Res] Abstract MiR-455-3p Regulates Early Chondrogenic Differentiation via Inhibiting Runx2 Investigators showed that miR-455-3p is increased in ATDC5 cells from 0 days to 21 days, but rapidly decreases at 28 days, and a similar expression kinetic is detected in the development of mouse embryos. They showed that miR-455-3p functions as an activator for early chondrogenic differentiation, most likely by inhibiting the expression of Runt-related transcription factor 2 (Runx2) as indicated by luciferase reporter assays. [FEBS Lett] Abstract Osteogenic Profile of Mesenchymal Cell Populations Contributing to Alveolar Bone Formation The authors investigated the osteogenic profile of mesenchymal cells dissected from mouse mandible slices at the stage of early alveolar bone formation. [Cells Tissues Organs] Abstract |