IN VITRO Urine of Preterm Neonates as a Novel Source of Kidney Progenitor Cells Neonatal stem/progenitor cells expressed markers of nephron progenitors but also, stromal progenitors, with many single cells coexpressing these markers. Furthermore, these cells presented mesenchymal stem cell features and protected cocultured tubule cells from cisplatin-induced apoptosis. [J Am Soc Nephrol] Abstract | Press Release MiR-708 Promotes Steroid-Induced Osteonecrosis of Femoral Head, Suppresses Osteogenic Differentiation by Targeting SMAD3 Researchers investigated the differential expression of miRs in the mesenchymal stem cells (MSCs) of patients with osteonecrosis of femoral head (ONFH), and aimed to explain the relationship between glucocorticoid use and the development of MSC dysfunction in ONFH. [Sci Rep] Full Article Characterization of Notch Signaling During Osteogenic Differentiation in Human Osteosarcoma Cell Line MG63 Scientists investigated, in vitro, the involvement of Notch pathway during all the developmental stages of osteogenic differentiation in human osteosarcoma cell line MG63. [J Cell Physiol] Abstract Loss-of-Function of HtrA1 Abrogates All-Trans Retinoic Acid-Induced Osteogenic Differentiation of Mouse Adipose-Derived Stromal Cells through Deficiencies in p70S6K Activation Investigators assessed the influence of small interfering RNA-induced repression of high temperature requirement protease A1 (HtrA1) production on mouse adipose-derived stromal cell osteogenesis and examined its effects on all-trans retinoic acid-mediated mammalian target of rapamycin signaling. [Stem Cells Dev] Abstract In Vitro Osteogenic Induction of Human Marrow-Derived Mesenchymal Stem Cells by PCL Fibrous Scaffolds Containing Dexamethazone-Loaded Chitosan Microspheres Researchers report the encapsulation of dexamethasone within the chitosan microspheres embedded in a fibrous structure of poly ɛ-caprolactone (PCL) to provide a platform for osteogenic differentiation of human mesenchymal stem cells. [J Biomed Mater Res A] Abstract Comparative Study of Equine Mesenchymal Stem Cells from Healthy and Injured Synovial Tissues: An In Vitro Assessment Investigators evaluated phenotypic characteristics and differentiation potential of equine mesenchymal stem cells from synovial fluid and synovial membrane of healthy joints, joints with osteoarthritis and joints with osteochondritis dissecans to determine the most suitable synovial source for an allogeneic therapy cell bank. [Stem Cell Res Ther] Full Article TLR4 Activation Promotes Bone Marrow MSC Proliferation and Osteogenic Differentiation via Wnt3a and Wnt5a Signaling Researchers found that activated TLR4 by its ligand LPS promoted the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro. TLR4 activation by LPS also increased cytokine IL-6 and IL-1β production in MSCs. [PLoS One] Full Article IN VIVO A Novel Therapeutic Approach Using Mesenchymal Stem Cells to Protect against Mycobacterium abscessus Scientists demonstrated the use of mesenchymal stem cells (MSCs) as a novel therapeutic strategy against M. abscessus, which is the most drug-resistant and difficult-to-treat mycobacterial pathogen. The systemic intravenous injection of MSCs not only improved mouse survival but also enhanced bacterial clearance in the lungs and spleen. [Stem Cells] Abstract In Vivo Immunomodulatory Effects of Adipose-Derived Mesenchymal Stem Cells Conditioned Medium in Experimental Autoimmune Encephalomyelitis The authors conducted a comparative analysis of the immunomodulatory properties of adipose tissue mesenchymal stem cells and their conditioned media, derived from C57/BL6 mice, for mitigating the adverse clinical course of experimental autoimmune encephalomyelitis. [Immunol Lett] Abstract |