Mesenchymal Cell News Volume 8.18 | May 10 2016

Ginsenoside Rg1 Promotes Osteogenic Differentiation of rBMSCs and Healing of Rat Tibial Fractures through Regulation of GR-Dependent BMP-2/SMAD Signaling
Researchers investigated the effect of Rg1 on osteogenic differentiation of cultured bone marrow mesenchymal stem cells (BMSCs) and related mechanisms and on the fracture healing in a fracture model. [Sci Rep] Full Article

Autonomous Magnetic Labelling of Functional Mesenchymal Stem Cells for Improved Traceability and Spatial Control in Cell Therapy Applications
The authors demonstrated efficient live mesenchymal stem cell (MSC) labelling using silica-coated magnetic particles, which enables 3D tracking and guidance of stem cells. A procedure developed for the efficient and unassisted particle uptake was shown to support MSC viability and integrity, while surface marker expression and MSC differentiation capability were also maintained. [J Tissue Eng Regen Med] Full Article

TGF-β3-Induced miR-494 Inhibits Macrophage Polarization via Suppressing PGE2 Secretion in Mesenchymal Stem Cells
Researchers found that miR-494 over-expressed decidual mesenchymal stem cells inhibit M2 macrophages polarization and this inhibitory effect is mediated by miR-494-reduced PGE2 secretion. [FEBS Lett] Abstract | Full Article

Establishment of a Cytocompatible Cell-Free Intervertebral Disc Matrix for Chondrogenesis with Human Bone Marrow-Derived Mesenchymal Stromal Cells
Scientists prepared a cell-free decellularized human intervertebral disc (IVD) scaffold and compared neotissue formation in response to recellularization with human IVD cells or human bone marrow-derived mesenchymal stromal cells. [Cells Tissues Organs] Full Article

Osteoblastic Differentiation of Bone Marrow Mesenchymal Stromal Cells in Bruck Syndrome
Mononuclear cells obtained from the bone marrow of BS, OI patients and healthy donors were cultured and osteogenic differentiation was induced. The gene expression of osteoblast specific markers were also evaluated during the osteoblastic differentiation of mesenchymal stem cells by qRT-PCR using an ABI7500 Sequence Detection System. [BMC Med Genet] Full Article

IN VIVO

E-Prostanoid 2 Receptor Overexpression Promoted Mesenchymal Stem Cell Attenuated Lung Injury
Near-infrared imaging and immunofluorescence both showed that compared with mesenchymal stem cells (MSCs)-GFP, MSCs-E-prostanoid 2 (EP2) significantly enhanced MSC homing to injured lung tissue. In vivo experimental results suggested that compared with the MSCs-GFP, the MSCs-EP2 further reduced LPS-induced pulmonary vascular permeability, as reflected by reduced levels of Evans blue in lung parenchymal homogenates. [Hum Gene Ther] Abstract

Preclinical Biosafety Evaluation of Genetically Modified Human Adipose Tissue–Derived Mesenchymal Stem Cells for Clinical Applications to Brainstem Glioma
Scientists injected a stereotactically low, medium, or high dose of human adipose tissue-derived mesenchymal stem cell secreted form of tumor necrosis factor-related apoptosis-inducing ligand (hAT-MSC.sTRAIL) into the brainstems of immune-deficient mice reflecting the plan of a future clinical trial. Local toxicity, systemic toxicity, secondary tumor formation, and bio-distribution of hAT-MSC.sTRAIL were investigated. [Stem Cells Dev] Abstract

Chm-1 Gene-Modified Bone Marrow Mesenchymal Stem Cells Maintain the Chondrogenic Phenotype of Tissue-Engineered Cartilage
The authors compared chondromodulin-1 (Chm-1) expression between chondrocytes and mesenchymal stem cells (MSCs). They found that chondrocytes expressed a high level of Chm-1. They then adenovirally transduced MSCs with Chm-1 and applied modified cells to engineer cartilage in vivo. [Stem Cell Res Ther] Full Article

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Cynata Enters License Option Agreement with apceth GmbH & Co. KG
Cynata Therapeutics Limited announced that it has entered into a worldwide license option agreement with apceth GmbH & Co. KG for the use of Cynata’s unique Cymerus technology in combination with genetic modification technologies designed to open up prospective new therapeutic fields to mesenchymal stem cells, particularly the treatment of cancer. [Cynata Therapeutics Limited (PR Newswire Association LLC)] Press Release

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NEW Postdoctoral Fellow – Tissue Regeneration (University of Pennsylvania)

NEW Postdoctoral Researcher – Stem Cell Biology, Regenerative Medicine (NUI Galway)

Research Technologist – Mesenchymal Stem Cell Biology (STEMCELL Technologies Inc.)

PhD Student Position – Bone Biology (KU Leuven)

Postdoctoral Position – Craniofacial Molecular Biology (University of Southern California)

Engineering Project Lead (Cytonome)

Postdoctoral Position – Epigenetic Regulation of Cell Fate, Cancer and Metabolism (University Medical Center Freiburg)

Postdoctoral Fellow – Mechanoregulation of Fibrosis and Vascular Injury (St. Michael’s Hospital)

Cell Culture Technician (California Institute of Technology)

Tenure-Track Faculty Positions – Assistant/Associate/Full Professor (Nanyang Technological University)

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