CARDIAC MUSCLE CELLS Multicolor Mapping of the Cardiomyocyte Proliferation Dynamics that Construct the Atrium To understand the structural divergence of the cardiac chambers, researchers determined the contributions of individual embryonic cardiomyocytes to the atrium in zebrafish by multicolor fate-mapping, and they compared their analysis to the established proliferation dynamics of ventricular cardiomyocytes. [Development] Abstract Selenium Augments MicroRNA Directed Reprogramming of Fibroblasts to Cardiomyocytes via Nanog Investigators report the identification of a specific media composition, reprogramming media (RM), which augmented the effect of a combination of microRNAs by 5–15-fold depending upon the cardiac marker tested. RM alone was sufficient to strongly induce cardiac gene and protein expression in neonatal tail-tip as well as cardiac fibroblasts. [Sci Rep] Full Article SKELETAL MUSCLE CELLS Optogenetic Skeletal Muscle-Powered Adaptive Biological Machines As a step toward forward engineering biological machines (bio-bots) capable of nonnatural functional behaviors, scientists created a modular light-controlled skeletal muscle-powered bioactuator that can generate up to 300 µN of active tension force in response to a noninvasive optical stimulus. [Proc Natl Acad Sci USA] Abstract | Press Release | Video L-Glutamine Improves Skeletal Muscle Cell Differentiation and Prevents Myotube Atrophy after Cytokine (TNF-α) Stress via Reduced p38 MAPK Signal Transduction As L-glutamine can dampen the effects of inflamed environments, scientists investigated the role of L-glutamine in both differentiating C2C12 myoblasts and existing myotubes in the absence/presence of tumor necrosis factor alpha (TNF-α) ± L-glutamine. [J Cell Physiol] Abstract Cell-Instructive Graphene-Containing Nanocomposites Induce Multinucleated Myotube Formation Investigators hypothesized that engineering biophysical features such as nano/micro-fibrous structure and conductive properties into a single biomaterial scaffold will instruct myoblasts to differentiate into multinucleated myotubes even in the absence of differentiation media. [Ann Biomed Eng] Abstract Chromatin-Wide and Transcriptome Profiling Integration Uncovers p38α MAPK as a Global Regulator of Skeletal Muscle Differentiation To investigate the global gene transcription program regulated by p38α during satellite cell differentiation, and to specifically address whether this regulation occurs through direct action of p38α on gene promoters, researchers performed a combination of microarray gene expression and genome-wide binding analyses. [Skelet Muscle] Abstract SMOOTH MUSCLE CELLS Equol Increases Cerebral Blood Flow in Rats via Activation of Large-Conductance Ca2+-Activated K+ Channels in Vascular Smooth Muscle Cells Outward K+ current in smooth muscle cells of cerebral basilar artery, large-conductance Ca2+-activated K+ (BK) channel current in BK-HEK 293 cells stably expressing both human α (hSlo)- and β1-subunits, and hSlo channel current in hSlo-HEK 293 cells expressing only the α-subunit of BK channels were recorded with whole cell patch-clamp technique. [Pharmacol Res] Abstract Unraveling the Role of Mechanical Stimulation on Smooth Muscle Cells: A Comparative Study between 2D and 3D Models Researchers investigated and compared the response of vascular smooth muscle cells cultured in different culture environments in combination with mechanical stimulation for two and five days. [Biotechnol Bioeng] Abstract Impact of Elastin Incorporation into Electrochemically Aligned Collagen Fibers on Mechanical Properties and Smooth Muscle Cell Phenotype Based on the principles of isoelectric focusing, two different types of elastin were incorporated into highly oriented electrochemically aligned collagen (ELAC) fibers and the effect of elastin incorporation on the mechanical properties of the ELAC fibers and smooth muscle cell phenotype was investigated. [Biomed Mater] Abstract |