CARDIAC MUSCLE CELLS Matricellular Protein CCN5 Reverses Established Cardiac Fibrosis Scientists examined the role of CCN5 in human heart failure and tested whether CCN5 can reverse established cardiac fibrosis in an experimental model of heart failure induced by pressure overload. [J Am Coll Cardiol] Full Article | Press Release | Podcast Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells Scientists demonstrated that they can expand cells with characteristics of cardiovascular progenitor cells from the mesenchymal stromal cell population of human fetal hearts. [Stem Cell Reports] Full Article | Graphical Abstract SKELETAL MUSCLE CELLS A Developmentally Regulated Switch from Stem Cells to Dedifferentiation for Limb Muscle Regeneration in Newts Researchers demonstrated that larval newts use stem/progenitor cells such as satellite cells for new muscle in a regenerated limb, whereas metamorphosed newts recruit muscle fiber cells in the stump for the same purpose. [Nat Commun] Full Article | Press Release Myofibroblasts Are Distinguished from Activated Skin Fibroblasts by the Expression of AOC3 and Other Associated Markers Scientists identified AOC3 (amine oxidase, copper containing 3), a surface monoamine oxidase, as a new marker of myofibroblasts by showing that it is the target protein of the myofibroblast-reacting mAb PR2D3. The normal and tumor tissue distribution and the cell line reactivity of AOC3 match that expected for myofibroblasts. [Proc Natl Acad Sci USA] Abstract Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors The authors developed an injectable 3D RGD-coupled alginate scaffold with multiple growth factor delivery capacity for encapsulating gingival mesenchymal stem cells (GMSCs), evaluated the capacity of encapsulated GMSCs to differentiate into myogenic tissue in vitro and in vivo where encapsulated GMSCs were transplanted subcutaneously into immunocompromised mice. [Ann Biomed Eng] Abstract Understanding the Role of ECM Protein Composition and Geometric Micropatterning for Engineering Human Skeletal Muscle Scientists investigated microenvironmental cues that drive myoblast differentiation into aligned myotubes for potential applications in skeletal muscle repair, organ-on-chip disease models and actuators for soft robotics. They used a 2D in vitro system to systematically evaluate the role of extracellular matrix (ECM) protein composition and geometric patterning for controlling the formation of highly aligned myotubes. [Ann Biomed Eng] Abstract SMOOTH MUSCLE CELLS Estradiol-17β Increases 12- and 15-Lipoxygenase (Type 2) Expression and Activity and Reactive Oxygen Species in Human Umbilical Vascular Smooth Muscle Cells Investigators tested the effects of estradiol-17β as well as estrogen-receptor subtype specific and non-specific agonists and antagonists on the expression and eicosanoid production of lipoxygenase enzymes expressed in culture human umbilical vascular smooth muscle cells. [J Steroid Biochem Mol Biol] Abstract Antagonists to Endothelin Receptor Type B Promote Apoptosis in Human Pulmonary Arterial Smooth Muscle Cells Cultured human pulmonary arterial smooth muscle cells (PASMCs) were treated with the endothelin A (ETA) receptor antagonist BQ-123, or the ETB antagonist A-192621 or BQ-788 for 48 hours. The viability of PASMCs was significantly decreased by A-192621 and BQ-788, in a dose-dependent manner. [Life Sci] Abstract miR-140-5p Regulates Hypoxia-Mediated Human Pulmonary Artery Smooth Muscle Cell Proliferation, Apoptosis and Differentiation by Targeting Dnmt1 and Promoting SOD2 Expression miR-140-5p is down-regulated in patients with pulmonary arterial hypertension (PAH) and experimental models of PAH, and inhibits hypoxia-mediated pulmonary artery smooth muscle cell proliferation in vitro. [Biochem Biophys Res Commun] Abstract |