CARDIAC MUSCLE CELLS Effects of Matrix Metalloproteinases on the Performance of Platelet Fibrin Gel Spiked with Cardiac Stem Cells in Heart Repair Investigators elucidated the effects of matrix metalloproteinase inhibition on the therapeutic benefits of intramyocardial injection of platelet fibrin gel spiked with cardiac stem cells in a rat model of acute myocardial infarction. [Stem Cells Transl Med] Abstract Induction of Human iPSC-Derived Cardiomyocyte Proliferation Revealed by Combinatorial Screening in High Density Microbioreactor Arrays Scientists used high-density microbioreactor arrays to combinatorially screen Wnt, Hedgehog, IGF and FGF pathway agonists. The Wnt activator CHIR99021 was identified as the most potent molecular inducer of human cardiomyocyte proliferation, inducing cell cycle activity marked by Ki67, and an increase in cardiomyocyte numbers compared to controls. [Sci Rep] Full Article Cardiac Non-Myocyte Cells Show Enhanced Pharmacological Function Suggestive of Contractile Maturity in Stem Cell Derived Cardiomyocyte Microtissues Researchers assessed the contribution of non-myocyte cells on the contractile function of co-cultured human embryonic stem cell derived cardiomyocytes in spheroid microtissue format. [Toxicol Sci] Abstract | Full Article Fibulin-2 Is Essential for Angiotensin II-Induced Myocardial Fibrosis Mediated by Transforming Growth Factor (TGF)-β Researchers investigated the role of fibulin-2 in the development of myocardial hypertrophy and fibrosis induced by continuous pressor-dosage of angiotensin II (Ang II) infusion. To test whether fibulin-2 was sufficient for Ang II-induced TGF-β upregulation, isolated fibulin-2 null cardiac fibroblasts were treated with Ang II after transfecting with fibulin-2 expression vector or pretreating with recombinant fibulin-2 protein. [Lab Invest] Abstract SKELETAL MUSCLE CELLS NAD+ Repletion Improves Mitochondrial and Stem Cell Function and Enhances Life Span in Mice Investigators demonstrated the importance of the amount of the oxidized form of cellular nicotinamide adenine dinucleotide (NAD+) and its impact on mitochondrial activity as a pivotal switch to modulate muscle stem cell senescence. [Science] Abstract | Press Release β-Catenin Activation in Muscle Progenitor Cells Regulates Tissue Repair The authors focus on the canonical Wnt signaling pathway and find that either conditional β-catenin disruption or activation in adult muscle stem cells results in perturbation of muscle regeneration. [Cell Rep] Full Article | Graphical Abstract Focal Adhesion Kinase and Paxillin Promote Migration and Adhesion to Fibronectin by Swine Skeletal Muscle Satellite Cells Researchers compared the migration and adhesion of skeletal muscle satellite cells derived from two species of pig in vitro, and explored how focal adhesion kinase signaling modulates the two processes. [Oncotarget] Full Article Lkb1 Deletion Upregulates Pax7 Expression through Activating Notch1 Signaling Pathway in Myoblasts Scientists demonstrated that deletion of liver kinase b1 (Lkb1) upregulates paired-box transcription factor 7 (Pax7) expression in myoblasts and inhibits asymmetric divisions that generate differentiating progenies. [Int J Biochem Cell Biol] Abstract Modeling Fibrosis Using Fibroblasts Isolated from Scarred Rat Vocal Folds Scientists pursued detailed characterization of scar vocal fold fibroblasts (VFFs) obtained from surgically injured rat VF mucosae, compared with those obtained from experimentally naïve, age-matched tissue. Scar VFFs initially exhibited a myofibroblast phenotype characterized by increased proliferation, increased Col1a1 transcription and collagen, type I synthesis, increased Acta2 transcription and α-smooth muscle actin synthesis, and enhanced contractile function. [Lab Invest] Abstract SMOOTH MUSCLE CELLS MicroRNA-223 Attenuates Hypoxia-Induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells Researchers identified that miR-223 was significantly downregulated in chronically hypoxic mouse and rat lungs, as well as in pulmonary artery and pulmonary artery smooth muscle cells (PASMC) exposed to hypoxia. Knockdown of miR-223 increased PASMC proliferation. [Sci Rep] Full Article Impaired Ca2+ Homeostasis and Decreased Orai1 Expression Modulates Arterial Hyporeactivity to Vasoconstrictors during Endotoxemia Researchers hypothesized that SIRS/endotoxemia-associated hyporesponsiveness to vasoconstrictors is mediated by smaller increases in intracellular Ca2+ levels due to reduced signaling via the STIM/Orai. Cultured vascular smooth muscle cell treated with LPS resulted in increased TLR-4 expression, but Myd-88 and STIM-1 expression were not changed. [Inflammation] Abstract |