Muscle Cell News 3.28 August 20, 2018 | |
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TOP STORYWhen human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) were encapsulated in a collagen-based hydrogel and seeded on the microstructured and mechanically tailored fiber scaffolds, they showed an increase in beating rate, enhanced cell alignment, sarcomere content and organization as well as an increase in cardiac maturation-related marker expression, indicative of enhanced iPSC-CM maturation, as compared to rudimentary fiber scaffolds. [Adv Funct Mater] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)CARDIAC MUSCLE CELLSScientists cultivated H9c2 cells in distinct conditions that resulted in either glycolytic or oxidative metabolism. Cell death occurred through a mechanism involving activation of a lysosomal-proteolytic degradation pathway. Accumulation of ceramide played a critical role in the susceptibility of glycolytic H9c2 cells to cytotoxicity. [Cell Death Discov] Full Article Researchers studied the impact of exocytic and post-endocytic trafficking on the cell surface channel abundance in cardiomyocytes. Single-molecule localization and confocal microscopy revealed an intracellular CaV1.2 pool tightly associated with microtubules from the perinuclear region to the cell periphery, and with actin filaments at the cell cortex. [iScience] Abstract | Full Article | Graphical Abstract SKELETAL MUSCLE CELLSScientists showed that a physiological increase in reactive oxygen species is required for satellite cells to exit the cell cycle and initiate differentiation through the redox activation of p38a MAP kinase. Subjecting cultured satellite cells to transient inhibition of P38a MAP kinase in conjunction with N-acetyl-cysteine treatment led to their rapid expansion, with striking improvement of their regenerative potential in grafting experiments. [Elife] Full Article In cultured murine and human skeletal myocytes, recombinant leptin increased VEGFA mRNA levels. Platelet-derived growth factor receptor (PDGFR)α− and PDGFRβ− expressing perivascular cell populations were identified as leptin producing within skeletal muscle of mice and humans. [Angiogenesis] Abstract COMP-Angiopoietin-1 Accelerates Muscle Regeneration through N-Cadherin Activation COMP-Ang1 directly bound to N-cadherin on the myoblast surface in a Ca2+ dependent manner. The interaction enhanced N-cadherin activation via N-cadherin/p120-catenin complex formation, which in turn activated p38MAPK and myogenin expression as well as increasing myogenin+ cells in/ex vivo. [Sci Rep] Full Article The authors noticed that oxidative-stress was reversed by sodium hydrogen sulfide (NaHS) in hyperhomocysteinemia (Hcy)-treated C2C12 cells. Endoplasmic reticulum-stress markers were elevated in vivo and in vitro, and NaHS mitigated these effects. They observed that JNK-phosphorylation was upregulated in C2C12 after Hcy treatment, but NaHS could not reduce this effect. [Am J Physiol Cell Physiol] Abstract Researchers engineered a synthetic hydrogel-based matrix with optimal mechanical, cell-adhesive, and protease-degradable properties that promoted muscle satellite cell survival, proliferation, and differentiation. [Sci Adv] Full Article | Press Release SMOOTH MUSCLE CELLSInvestigators described an innovative method allowing smooth muscle actin (SMA) positive vascular smooth muscle cells and SMA− mid-capillary pericytes to be freshly isolated from the rat cerebral cortex. Using differential RNA-Seq analysis, they then revealed the specific gene expression profile of each subtype. [Sci Rep] Full Article DNA methyltransferase inhibitor, 5-aza-2-deoxycytidine treatment increased BDNF expression in bladder smooth muscle cells (SMCs). Stretch-induced BDNF was inhibited by the YAP/WWTR1 inhibitor Verteporfin. Verteporfin improved the SMC phenotype in part modulated by reducing BDNF. [Am J Pathol] Abstract Epigenetic Modification of Intestinal Smooth Muscle Cell Phenotype during Proliferation Exposure to the HDAC inhibitor trichostatin A or 5-azacytidine, a DNMT inhibitor, reversed the characteristic loss of phenotypic markers among high passage cell lines of intestinal smooth muscle cells. Expression of smooth muscle actin and SM-22 were markedly increased, as well as functional expression of the neurotrophin GDNF. [Am J Physiol Cell Physiol] Abstract Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & ESC & iPSC News. | |
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REVIEWSSkeletal Muscle Mitochondrial Remodeling in Exercise and Diseases Recent studies have revealed a finely tuned regulatory network that orchestrates skeletal muscle mitochondrial biogenesis and function in response to exercise and in disease states. The authors review the current state of knowledge relevant to the dynamic remodeling of skeletal muscle mitochondria in response to exercise and in disease states. [Cell Res] Abstract The Hippo Pathway in the Heart: Pivotal Roles in Development, Disease, and Regeneration Investigators highlight the roles of the Hippo-Yes-associated protein (YAP) pathway in endogenous heart muscle renewal, including the pivotal role of the Hippo-YAP pathway in regulating cardiomyocyte proliferation and differentiation, stress response, and mechanical signaling. [Nat Rev Cardiol] Abstract Visit our reviews page to see a complete list of reviews in the muscle cell research field. | |
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INDUSTRY NEWSIn an effort to fulfill this unmet medical need, AbCellera Biologics Inc. has entered into a three-year partnership with Drs. Fabio Rossi and Michael Underhill of the University of British Columbia to discover, test, and develop therapeutic antibodies for the treatment of Duchenne Muscular Dystrophy-associated fibrosis. [AbCellera Biologics Inc.] Press Release Leadiant Biosciences, Inc.announced it has entered into a license agreement and cooperative research and development agreement with the National Institutes of Health to develop oral N-acetyl-D-mannosamine for the potential treatment of individuals with GNE myopathy. [Leadiant Biosciences, Inc.] Press Release Wave Life Sciences Receives US Orphan Drug and Rare Pediatric Disease Designations for WVE-210201 Wave Life Sciences Ltd. announced that the U.S. FDA has granted both orphan drug designation and rare pediatric disease designation for WVE-210201 for the treatment of Duchenne muscular dystrophy. The European Commission previously granted orphan drug designation for WVE-210201 in July 2018. [Wave Life Sciences] Press Release | |
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POLICY NEWSSalk Institute Asks Judge to Narrow Scope of Gender-Discrimination Suit Lawyers representing the Salk Institute for Biological Studies went to court on 17 August in San Diego, California, asking a judge to narrow the scope of a gender-discrimination lawsuit filed by molecular biologist Beverly Emerson. [Nature News] Editorial Prominent Health Policy Researcher Plagiarized Colleagues’ Work, Dartmouth Investigation Finds A Dartmouth College investigation has concluded that Dr. H. Gilbert Welch, one of the country’s most prominent health care policy scholars, committed research misconduct in connection with a paper published in a top medical journal. [STAT News] Editorial Top Geneticist Loses £3.5-Million Grant in First Test of Landmark Bullying Policy One of the world’s largest research-funding charities has revoked a £3.5-million (US$4.5-million) grant awarded to a top cancer geneticist, Nazneen Rahman, following allegations that she bullied scientists and other staff when she worked at the Institute of Cancer Research in London. [Nature News] Editorial The NIH Loosens Grip on Gene Therapy Trials The directors of the National Institutes of Health (NIH) and the US FDA propose limiting the role of the NIH in assessing proposals for gene therapy experiments, the two explain in a commentary in the New England Journal of Medicine. [The Scientist] Editorial
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EVENTSNEW The New York Stem Cell Foundation (NYSCF) Conference Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Position – Duchenne Muscular Dystrophy Research (Leiden University Medical Center) Scientist – Cardiovascular Research (University of Iowas Hospital and Clinics) Postdoctoral Position – Duchenne Muscular Dystrophy Research (Genethon) Laboratory Research Scientist – Left Ventricle Heart Development (The Francis Crick Institute) Postdoctoral Research Fellow – Cardiac Cells (University of British Columbia) Postdoctoral Associate – Muscle Cell Research (University of Louisville) Postdoctoral Fellow – Immunoengineering for Muscle Regeneration (Duke University) Postdoctoral Fellow – Cell-Based Cardiac Regeneration (Karolinska Institutet) Postdoctoral Fellow – Human Skeletal Muscle Disease Modeling and Regeneration (Duke University) Postdoctoral Fellow – hiPSC Based Cardiac Regeneration (Duke University) Postdoctoral Fellow – Cardiovascular Tissue Engineering (Stanford University) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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