Muscle Cell News 4.08 March 11, 2019 | |
| |
TOP STORYUsing human induced-pluripotent-stem-cell-derived cardiomyocytes (hiPSC-CMs) and mitogen-activated protein kinase kinase kinase kinase-4 (MAP4K4) gene silencing, scientists demonstrated that death induced by oxidative stress required MAP4K4. They devised a small-molecule inhibitor, DMX-5804, that rescued cell survival, mitochondrial function, and calcium cycling in hiPSC-CMs. [Cell Stem Cell] Full Article | Press Release | Graphical Abstract | |
| |
PUBLICATIONS(Ranked by impact factor of the journal)CARDIAC MUSCLE CELLSThe Long Non-Coding RNA CPR Regulates Cardiomyocyte Proliferation and Cardiac Repair Cardiomyocyte Proliferation Regulator (CPR) level is comparatively higher in the adult heart than in the fetal stage. The silencing of CPR significantly increased the cardiomyocyte proliferation in the postnatal and adult hearts. CPR deletion restored the heart function after myocardial injury, which was evident from increased cardiomyocyte proliferation, improvement of myocardial function and reduced scar formation. [Circulation] Abstract Transgenic induced pluripotent stem cell (iPSC) lines with either Oct4 or α-myosin heavy chain promoter driving green fluorescent protein (GFP) expression were used to study cardiomyocyte differentiation in real microgravity. The differentiation status was monitored by GFP fluorescence intensity. [Stem Cells Dev] Full Article | Press Release SKELETAL MUSCLE CELLSCRISPR-Cas9 Corrects Duchenne Muscular Dystrophy Exon 44 Deletion Mutations in Mice and Human Cells Scientists present a simple and efficient strategy for correction of exon 44 deletion mutations by CRISPR-Cas9 gene editing in cardiomyocytes obtained from patient-derived induced pluripotent stem cells and in a new mouse model harboring the same deletion mutation. [Sci Adv] Full Article In an in vitro model of skeletal muscle atrophy, differentiated C2C12 cells exhibited reduced Akt activity and decreased FOXO1 phosphorylation, a process known to promote transcription of atrophy genes in skeletal muscle. [Sci Rep] Full Article SMOOTH MUSCLE CELLSResearchers describe methods for determining real-time intracellular Ca2+ release and contraction in vascular smooth muscle cells of neural crest origin differentiated from human induced pluripotent stem cells using multiple protocols, and compared these with primary human brain vascular pericytes and smooth muscle cells. [Stem Cell Reports] Full Article | Graphical Abstract Isolation and Characterization of Myogenic Precursor Cells from Human Cremaster Muscle Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. [Sci Rep] Full Article The authors demonstrated increased expression of death-associated protein kinase 3 in the aortas of vascular calcificatio rats and vascular smooth muscle cell (VSMCs) cultured in calcifying media. Knockdown of death-associated protein kinase 3 significantly inhibited calcifying media-induced VSMC mineralization and retarded the phenotypic transformation of VSMCs into osteogenic cells. [Eur J Pharmacol] Abstract | Graphical Abstract Advanced bioinformatics analyses indicated that the upregulated mRNAs were mainly involved in inflammatory responses, lipid metabolism, clearance of amyloid-β peptide, citrate cycle, TGF-β signaling, and chemokine signaling. The downregulated mRNAs were mainly involved in the apoptosis pathway, glycerolipid metabolism, Wnt signaling pathway, and MAPK signaling pathway. [Biosci Rep] Full Article Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & ESC & iPSC News. | |
| |
REVIEWSRegulation of Smooth Muscle Cell Proliferation by NADPH Oxidases in Pulmonary Hypertension The authors review recent data supporting a role for reactive oxygen species generated from nicotinamide adenine dinucleotide phosphate (NADPH) oxidases in promoting pulmonary arterial smooth muscle cell proliferation during pulmonary hypertension. [Antioxidants] Full Article Visit our reviews page to see a complete list of reviews in the muscle cell research field. | |
| |
INDUSTRY NEWSBaxter Announces U.S. FDA Approval and Launch of Ready-to-Use Cardiovascular Medicine Eptifibatide Baxter International Inc. announced the U.S. FDA approval and launch of ready-to-use eptifibatide. [Baxter International Inc.] Press Release STEMCELL Technologies Wins Deloitte Best Managed Companies Award STEMCELL Technologies announced that the company has been selected as a winner of Canada’s Best Managed Companies program for 2019. Canada’s Best Managed Companies is the country’s leading business awards program, recognizing excellence in the management abilities and practices of Canadian owned and managed companies with revenues over $15 million. [STEMCELL Technologies Inc.] Press Release | |
| |
POLICY NEWSTrump Once Again Requests Deep Cuts in U.S. Science Spending For the third year in a row, President Donald Trump’s administration has unveiled a budget request to Congress that calls for deep spending cuts at many federal science agencies, including a 15% cut for the National Institutes of Health and a 12% cut for the National Science Foundation, while providing hefty increases for the military. [ScienceInsider] Editorial Study Finds Gender Imbalances in First-Time NIH Awards Women receiving their first research grant from the National Institutes of Health are awarded an average of $39,000 less than men, according to an analysis published in JAMA. [The Scientist] Editorial
| |
EVENTSNEW European Muscle Conference 2019 Visit our events page to see a complete list of events in the community.
| |
JOB OPPORTUNITIESNEW Postdoctoral Research Associate – Cardiovascular Research (Old Dominion University) Postdoctoral Position – Mechanotransduction in Heart Development and Regeneration (Brown University) Assistant Professor – Sarcoma or Skeletal Related Malignancies (University of California, Davis) Postdoctoral Position – Muscle Stem Cell Biology (University of Sherbrooke) Postdoctoral Fellow – Stem Cells & Cardiac Regeneration (Temple University) Assistant Specialist – Cardiovascular Research (University of California, Davis) Postdoctoral Research Associate – Cardiac Stem Cells (University of Louisville) Postdoctoral Research Position – Cardiovascular Medicine (University of California, Davis) Assistant/Associate Adjunct Professor – Cardiovascular Medicine (University of California Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
| |
Have we missed an important article or publication in Muscle Cell News? Click here to submit! Comments or suggestions? Submit your feedback here. | |
|