Muscle Cell News 4.13 April 15, 2019 | |
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TOP STORYNeonatal ventricular myocytes (NRVMs) treated with miR-294 showed elevated expression of Ki67, p-histone H3 and Aurora B confirmed by immunocytochemistry compared to control cells. miR-294 enhanced oxidative phosphorylation and glycolysis in NRVMs measured by seahorse assay. Mechanistically, miR-294 repressed Wee1 leading to increased activity of the cyclin B1/CDK1 complex confirmed by qRT-PCR and immunoblot analysis. [Circ Res] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)CARDIAC MUSCLE CELLS3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts A biopsy of an omental tissue was taken from patients. While the cells were reprogrammed to become pluripotent stem cells, and differentiated to cardiomyocytes and endothelial cells, the extracellular matrix was processed into a personalized hydrogel. The two cell types were separately combined with hydrogels to form bioinks for the parenchymal cardiac tissue and blood vessels. [Adv Sci] Abstract | Editorial Engineering Microenvironment for Human Cardiac Tissue Assembly in Heart-on-a-Chip Platform Utilizing a novel, plastic and open-access heart-on-a-chip system that is capable of continuous non-invasive monitoring of tissue contractions, scientists were able to study how different micro-environmental cues affected the assembly of the cardiomyocytes into a functional cardiac tissue. [Matrix Biol] Abstract | Graphical Abstract Biphasic and Cardiomyocyte-Specific IFIT Activity Protects Cardiomyocytes from Enteroviral Infection Using IFN-induced with tetratricopeptide (IFIT) locus knockout cardiomyocytes the authors showed that, in the absence of the IFIT locus, viral replication was dramatically increased, indicating that constitutive IFIT expression suppresses type B coxsackievirus replication in this cell type. [PLoS Pathog] Full Article Inflammatory CD11b/c cells triggered a store-independent calcium influx in hypertrophied cardiomyocytes, that was mimicked by TNFα. Pharmacological or molecular approaches demonstrated that this calcium influx, depended on TNFR2, was Orai3-driven, and elicited cardiomyocyte hypertrophy and resistance to oxidative stress. [Sci Rep] Full Article SKELETAL MUSCLE CELLSInvestigators revealed that Yin Yang1 (YY1) bound and repressed mitochondrial gene expression. Simultaneously, it also stabilized Hif1α protein and activated Hif1α-mediated glycolytic genes to facilitate a metabolic reprogramming toward glycolysis which was needed for skeletal muscle satellite cell proliferation. [EMBO J] Abstract | Graphical Abstract Researchers screened a candidate circular RNA (circRNA) derived from the SNX29 gene, termed circSNX29 from their previous circRNAs sequencing data of bovine skeletal muscle, and further characterized its regulation and function during muscle development. The overexpression of circSNX29 facilitated myoblast differentiation and inhibited cell proliferation. [Mol Ther Nucleic Acids] Abstract | Full Article Scientists demonstrated that treating Murphy Roths Large (MRL/MpJ) muscle-derived stem/progenitor cells with dimethyloxalylglycine and CoCl2 increased the expression of HIF-1α and target genes, including angiogenic and cell survival genes. They also observed that HIF-1α activated the expression of paired box (Pax)7 through direct interaction with the Pax7 promoter. [FASEB J] Abstract TBX3 Represses TBX2 under the Control of the PRC2 Complex in Skeletal Muscle and Rhabdomyosarcoma The authors showed that TBX3 directly repressed TBX2 in rhabdomyosarcoma and skeletal muscle. TBX3 overexpression impaired cell growth and migration and they showed that TBX3 was directly repressed by the polycomb repressive complex 2 (PRC2), which methylates histone H3 lysine 27. [Oncogenesis] Abstract SMOOTH MUSCLE CELLSC60 Fullerenes Selectively Inhibit BKCa but Not Kv Channels in Pulmonary Artery Smooth Muscle Cells Researchers showed both computationally and experimentally that water-soluble pristine C60 fullerene strongly inhibited the large conductance Ca2+-dependent K+ (BKCa), but not voltage-gated K+ (Kv) channels in pulmonary artery smooth muscle cells. Both molecular docking simulations and analysis of single channel activity indicated that C60 fullerene blocked BKCa channel pore in its open state. [Nanomedicine] Abstract | Graphical Abstract mTOR Controls Embryonic and Adult Myogenesis via mTORC1 The authors showed that inactivation of mTOR complex (mTORC)1, but not mTORC2, in developing muscle caused perinatal death. In the adult, mTORC1 deficiency in muscle stem cells greatly impinged on injury-induced muscle regeneration. These phenotypes were because of defects in the proliferation and fusion capacity of the targeted muscle progenitors. [Development] Abstract Long Noncoding RNAs in Vascular Smooth Muscle Cells Regulate Vascular Calcification Investigators showed that calcium deposition was elevated after knockdown of Lrrc75a-as1, while its overexpression inhibited calcium accumulation in A10 cells. In addition, Lrrc75a-as1 attenuated vascular smooth muscle cell calcification by decreasing the expression of osteoblast-related factors. [Sci Rep] Full Article Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & ESC & iPSC News. | |
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REVIEWSAdult Stem Cells at Work: Regenerating Skeletal Muscle The authors summarize the process of regeneration with a specific emphasis on signaling pathways, cytoskeletal rearrangements, the importance of miRNAs, and the contribution of non-satellite cells such as immune cells, fibro-adipogenic progenitor cells, and PW1-positive/Pax7-negative interstitial cells. [Cell Mol Life Sci] Full Article Supporting the Heart: Functions of the Cardiomyocyte’s Non-Sarcomeric Cytoskeleton Investigators discuss the current state of cytoskeletal biology in cardiomyocytes, as well as research from other cell types, that together suggest there is a wealth of knowledge on cardiac health and disease waiting to be uncovered through exploration of the complex signaling networks of cardiomyocyte non-sarcomeric cytoskeletal proteins. [J Mol Cell Cardiol] Abstract Visit our reviews page to see a complete list of reviews in the muscle cell research field. | |
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SCIENCE NEWSSanthera to Present Long-Term Efficacy Results with Idebenone in DMD Santhera Pharmaceuticals and its collaborating clinical experts present results of long-term efficacy on respiratory function outcomes in idebenone-treated patients with Duchenne muscular dystrophy (DMD). [Press release from Santhera Pharmaceuticals discussing research presented at the 2019 Muscular Dystrophy Association (MDA) Clinical and Scientific Conference, Orlando] Press Release Biogen Continues to Advance Innovation in SMA with New Data in Adults and Infants to Be Presented Biogen announced it will present new data illustrating the rapidly progressive nature of spinal muscular atrophy (SMA) in adults, adolescents and older children. [Press release from Biogen discussing research presented at the 2019 Muscular Dystrophy Association (MDA) Clinical and Scientific Conference, Orlando] Press Release Amicus Therapeutics to Present Preclinical Pompe Disease Gene Therapy Data Amicus Therapeutics announced the company will present initial preclinical data from its investigational adeno-associated viral gene therapy program for Pompe disease in a poster. [Press release from Amicus Therapeutics discussing research to be presented at the American Society of Gene & Cell Therapy (ASGCT) 22nd Annual Meeting, Washington DC] Press Release | |
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INDUSTRY NEWSDNAnexus, Inc., and the not-for-profit Sutter Health network announced the first large-scale clinico-genomic multiple sclerosis research study conducted by a leading healthcare system. The Genome Center at UPMC will generate clinical-grade genomic data from samples contributed by the program’s participants. [DNAnexus, Inc. (Business Wire, Inc.)] Press Release Acceleron Discontinues Development of Phase I Molecule ACE-2494 Acceleron Pharma Inc. announced it is discontinuing development of ACE-2494, a systemic muscle agent the company had been studying in a Phase I healthy volunteer trial for the potential treatment of neuromuscular disorders. [Acceleron Pharma Inc.] Press Release FibroGen, Inc. announced that the FDA has granted Orphan Drug Designation for the company’s anti-CTGF antibody, pamrevlumab, for the treatment of patients with Duchenne muscular dystrophy. [FibroGen, Inc.] Press Release Sorrento Therapeutics, Inc. announced that its Neuro-Therapeutics unit is pursuing clinical development of RTX for multiple life-threatening cardiovascular disorders, including IND enabling toxicology and proof-of-principle animal studies. [Sorrento Therapeutics, Inc.] Press Release | |
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POLICY NEWSIndonesian Mega-Science Agency in the Balance as Election Begins Indonesians will vote in a national election that pits two old rivals against each other. The stakes are high for science. President Joko Widodo says that if he is re-elected, he will overhaul how much of the country’s research is organized and funded. The main opposition candidate has been silent on science so far. [Nature News] Editorial Universities Will Soon Announce Action against Scientists Who Broke NIH Rules, Agency Head Says Some US universities will announce in the next week or two actions they have taken to prevent foreign governments from taking unfair advantage of research funded by the National Institutes of Health (NIH) in Bethesda, Maryland, NIH Director Francis Collins said. Some researchers could be fired, Collins suggested. [ScienceInsider] Editorial
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EVENTSNEW European CanCer Organisation (ECCO) 2019: European Cancer Summit Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Senior Research Investigator – Multiple Sclerosis Research (Thomas Jefferson University) International Postdoctoral Fellowships – Metabolism (University of Copenhagen) Postdoctoral Scholar – Cardiovascular Genomics (Stanford Medicine) Early Stage Researchers – Various (Institutions across Europe in the TRIM-NET – ITN Network) Postdoctoral Research Associate – Cardiovascular Research (Old Dominion University) Postdoctoral Position – Mechanotransduction in Heart Development and Regeneration (Brown University) Assistant Professor – Sarcoma or Skeletal Related Malignancies (University of California, Davis) Assistant Specialist – Cardiovascular Research (University of California, Davis) Assistant/Associate Adjunct Professor – Cardiovascular Medicine (University of California, Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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