Muscle Cell News Volume 5.04 | Feb 3 2020

TOP STORY

Isolation of Satellite Cells and Transplantation into Mice for Lineage Tracing in Muscle

Investigators provide an optimized protocol for muscle stem cell isolation and transplantation into mice to deconvolute heterogeneity within isolated stem cell populations. [Nat Protoc] Abstract

(Ranked by impact factor of the journal)

CARDIAC MUSCLE CELLS

Modeling the Transport of Nuclear Proteins along Single Skeletal Muscle Cells

Scientists used a mosaic transfection model to create myotubes that contained exactly one myonucleus expressing a fluorescent nuclear reporter and monitored its distribution among all myonuclei. They found that the transport properties of these model nuclear proteins in myotubes depended on molecular weight and nuclear import rate, as well as on myotube width. [Proc Natl Acad Sci USA] Full Article

Reference Glycan Structure Libraries of Primary Human Cardiomyocytes and Pluripotent Stem Cell-Derived Cardiomyocytes Reveal Cell-Type and Culture Stage-Specific Glycan Phenotypes

Researchers used porous graphitized carbon liquid chromatography interfaced with mass spectrometry to generate glycan structure libraries for primary human heart tissue homogenate, cardiomyocytes (CMs) enriched from human heart tissue, and human induced pluripotent stem cell derived CMs. [J Mol Cell Cardiol] Abstract | Graphical Abstract

Systematic Comparison of High-Throughput Single-Cell and Single-Nucleus Transcriptomes during Cardiomyocyte Differentiation

To systematically assess the differences between high-throughput single-cell and single-nuclei RNA-seq approaches, the authors compared Drop-seq and DroNc-seq, two microfluidic-based 3′ RNA capture technologies that profile total cellular and nuclear RNA, respectively, during a time course experiment of human induced pluripotent stem cells differentiating into cardiomyocytes. [Sci Rep] Full Article

SKELETAL MUSCLE CELLS

Insulin-Independent Stimulation of Skeletal Muscle Glucose Uptake by Low-Dose Abscisic Acid via AMPK Activation

Scientists investigated the mechanism through which low-dose abscisic acid (ABA) controls glycemia and glucose fate. ABA stimulated uptake of the fluorescent glucose analog 2-NBDG by L6, and of [¹⁸F]-deoxy-glucose by mouse skeletal muscle, in the absence of insulin, and both effects were abrogated by the specific AMPK inhibitor dorsomorphin. [Sci Rep] Full Article

Effects of Mechanical Trauma on the Differentiation and ArfGAP3 Expression of C2C12 Myoblast and Mouse Levator Ani Muscle

The authors explored the effects of mechanical stress on myoblasts and levator ani muscle at the cellular and animal level and the possible mechanism of pelvic floor dysfunction induced by mechanical trauma. [Int Urogynecol J] Abstract

SMOOTH MUSCLE CELLS

Pro-Elastogenic Effects of Mesenchymal Stem Cell Derived Smooth Muscle Cells in a 3D Collagenous Milieu

Scientists investigated the ability of bone marrow mesenchymal stem cell-derived smooth muscle cells (SMCs) to maintain superior elastogenic properties in a 3D collagenous milieu alone and in co-culture with aneurysmal rat aortic SMCs to evaluate their potential as an alternative cell source for cell therapy in abdominal aortic aneurysm. [Acta Biomater] Abstract | Graphical Abstract

Slug, a Cancer‐Related Transcription Factor, Is Involved in Vascular Smooth Muscle Cell Transdifferentiation Induced by Platelet‐Derived Growth Factor‐BB during Atherosclerosis

Researchers showed that the platelet‐derived growth factor‐BB-dependent mechanism of Slug stabilization in vascular smooth muscle cells involved the extracellular signal‐regulated kinase 1/2 pathway. [J Am Heart Assoc] Full Article

Total Panax notoginseng Saponin Inhibits Vascular Smooth Muscle Cell Proliferation and Migration and Intimal Hyperplasia by Regulating WTAP/p16 Signals via m⁶A Modulation

Investigators illustrated that total Panax notoginseng saponin (TPNS) inhibited the viability, proliferation, and migration potential of vascular smooth muscle cells (VSMCs) via promotion of WT1 associated protein (WTAP) expression and suppression of WTAP restored the TPNS-induced inhibition of cell viability, proliferation and migration potential of VSMCs. [Biomed Pharmacother] Full Article

Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & ESC & iPSC News.

Impact of Melatonin on Skeletal Muscle and Exercise

The authors discuss the most recent evidence of dietary melatonin efficacy in age-related skeletal muscle disorders in cellular, pre-clinical, and clinical studies. Furthermore, they analyze the emerging impact of melatonin on physical activity. [Cells] Abstract | Graphical Abstract | Download Full Article

Visit our reviews page to see a complete list of reviews in the muscle cell research field.

Osaka University Transplants iPS Cell-Based Heart Cells in World’s First Clinical Trial

An Osaka University team said it has carried out the world’s first transplant of cardiac muscle cells created from iPS cells in a physician-initiated clinical trial. [The Japan Times] Editorial

Roche’s Risdiplam Meets Primary Endpoint in Pivotal FIREFISH Trial in Infants with Type 1 Spinal Muscular Atrophy

Roche announced positive topline results from the pivotal Part 2 of the FIREFISH study, evaluating risdiplam in infants aged one-seven months with Type 1 spinal muscular atrophy. [F. Hoffmann-La Roche Ltd] Press Release

Cash From Cannabis Companies Creates Conflicted Researchers

Imagine that scientists charged with doing research on tobacco’s health implications were funded by tobacco companies. In fact, the tobacco industry used this tactic for decades to cast doubt on the adverse health effects of smoking. But today it would be an outrageous conflict of interest – research on addictive drugs shouldn’t be paid for by people who stand to profit from selling them. Yet this is exactly what is happening with cannabis. [STAT News] Editorial

Chan Zuckerberg Initiative to Fund 30 Patient Groups, Aiming to Build a Model for Tackling Rare Diseases

On January 3^rd, Mark Zuckerberg and his spouse Dr. Priscilla Chan’s philanthropic vehicle announced a set of grants that executives there hope will create a model for tackling any rare disease: financing patient-led groups, and giving them training and resources. [STAT News] Editorial

NEW 10^th International Meeting of the Stem Cell Network North Rhine-Westphalia
June 8-9, 2020
Aachen, Germany

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Fellow – Vascular Biology (Augusta University)

NEW Postdoctoral Position – Vascular Smooth Muscle and Endothelial Cell Ion Channels (University of Tennessee Health Science Center)

Academic Clinical Lecturer – Clinical Pharmacology and Therapeutics Cardiology (University of Bristol)

Postdoctoral Position – Molecular Skeletal Muscle Cell Signaling (University of Copenhagen)

Postdoctoral Fellow – Cardiac Tissue Engineering and Regeneration (Duke University)

Postdoctoral Research Associate – Muscle Stem Cell Biology (State University of New York)

PhD Student – Aortic Disease (University Hospital of Cologne)

Postdoctoral Research Fellow/Postdoctoral Scientist – Aortic Disease (University Hospital of Cologne)

Postdoctoral Fellow – Computation Systems and Skeletal Muscles (University of Luxembourg)

Postdoctoral Researcher – Muscle Stem Cell Biology (University at Albany)

Assistant Specialist – Cardiovascular Medicine (University of California, Davis)

Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.

Have we missed an important article or publication in Muscle Cell News? Click here to submit!

Comments or suggestions? Submit your feedback here.