Muscle Cell News Volume 5.07 | Mar 02 2020

TOP STORY

Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration

Inactivating Lamin B2 (LMNB2) in human iPS cell-derived cardiomyocytes reduced karyokinesis and increased formation of polyploid nuclei. In primary cardiomyocytes from human infants with heart disease, modifying LMNB2 expression correspondingly altered metaphase progression and ploidy of daughter nuclei. [Dev Cell] Full Article | Graphical Abstract

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CARDIAC MUSCLE CELLS

Aberrant Expression of a Non-Muscle RBFOX2 Isoform Triggers Cardiac Conduction Defects in Myotonic Dystrophy

Scientists report that upregulation of a non-muscle splice isoform of RNA-binding protein RBFOX2 in myotonic dystrophy type 1 (DM1) heart tissue, due to altered splicing factor and microRNA activities, induced cardiac conduction defects in DM1 individuals. [Dev Cell] Full Article | Graphical Abstract

High-Mobility Group AT-Hook 1 Promotes Cardiac Dysfunction in Diabetic Cardiomyopathy via Autophagy Inhibition

Overexpression of high-mobility group AT-hook1 (HMGA1) accelerated high-glucose-induced cardiomyocyte inflammation and apoptosis, while HMGA1 knockdown relieved inflammation and apoptosis in cardiomyocytes in response to high glucose. [Cell Death Dis] Full Article

Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity

A 3D cardiac microtissue model was developed with engineered mechanical nonuniformity, enabled by 3D-printed hybrid matrices composed of fibers with different diameters. [Adv Healthc Mater] Abstract

Insulin-Like Growth Factor-II Overexpression Accelerates Parthenogenetic Stem Cell Differentiation into Cardiomyocytes and Improves Cardiac Function after Acute Myocardial Infarction in Mice

Scientists showed that the expression of cardiac troponin T and troponin I in insulin-like growth factor-II (IGF-II)-parthenogenetic stem cell (PSC) outgrowths preceded that of parental PSC outgrowths, suggesting that IGF-II could accelerate PSC differentiation into cardiac lineage. [Stem Cell Res Ther] Full Article

SIRT3-Mediated Inhibition of FOS Through Histone H3 Deacetylation Prevents Cardiac Fibrosis and Inflammation

Researchers investigated the role of sirtuin 3 (SIRT3) in inflammation and fibrosis in the heart using male mice with constitutive and systemic deletion of SIRT3 and human cardiac AC16 cells. [Signal Transduct Target Ther] Full Article

SKELETAL MUSCLE CELLS

Regulation of Proteins in Human Skeletal Muscle: The Role of Transcription

Investigators found that proteins with different functions were regulated differently at the transcriptional level, while an increase in the content of mitochondrial proteins was not. [Sci Rep] Full Article

Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis

Reseachers isolated and characterized satellite cells obtained from human biopsies and established an in vitro model of myogenesis, evaluating muscle regeneration, monitoring the dynamic increases of the specific myogenic regulatory factors and the final formation of multinucleated myofibers. [Calcif Tissue Int] Full Article

Inhibition of Protein Phosphatase Methylesterase 1 Dysregulates MAP Kinase Signaling and Attenuates Muscle Cell Differentiation

Inhibition of Protein phosphatase methylesterase 1 (Ppme1) by ABL127 led to a significant increase in AP-1 reporter activity, as well as, increases in ERK1/2, c-Jun, Ppme1, and PP2A protein levels in differentiating muscle cells. [Gene] Abstract

SMOOTH MUSCLE CELLS

The GEF Cytohesin-2/ARNO Mediates Resistin induced Phenotypic Switching in Vascular Smooth Muscle Cells

Secin H3 treatment, as well as expression of an inactive ARNO, reduced resistin induced vascular smooth muscle cell synthetic activity, as assessed by matrix metalloproteinase 2 expression, as well as the migration into a wound in vitro compared to ARNO WT expression. [Sci Rep] Full Article

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Sirtuin-1 and Its Relevance in Vascular Calcification

Dysregulation of endothelial cells and vascular smooth muscle cells is related to vascular calcification. Sirtuin-1 deacetylase encompasses a broad range of transcription factors that are linked to an extended lifespan. [Int J Mol Sci] Full Article

Visit our reviews page to see a complete list of reviews in the muscle cell research field.

CYTOO and AskBio Enter Research Agreement to Screen Gene Therapy Candidates for Rare Muscle Disorder

CYTOO, Inc. announced that it has entered into a research collaboration aimed at selecting a gene therapy candidate for a rare muscle disorder with Asklepios BioPharmaceutical, Inc. Under the terms of the agreement, AskBio and CYTOO will work together to develop an AAV-based screening platform derived from patient cells. [CYTOO, Inc.] Press Release

Axcella Completes Enrollment of Subjects in AXA1665-002 Study

Axcella, Inc. announced that it has completed subject enrollment in its ongoing AXA1665-002 clinical study. A comprehensive panel of biomarkers are being measured related to amino acid metabolism, ammonia, muscle structure and function including body composition, and neurocognition. [Axcella, Inc.] Press Release

China Bans Cash Rewards for Publishing Papers

Chinese institutions have been told to stop paying researchers bonuses for publishing in journals, as part of a new national policy to cut perverse incentives that encourage scientists to publish lots of papers rather than focus on high-impact work. [Nature News] Editorial

UC Santa Cruz Fires Graduate Students Taking Part in Strike

The University of California, Santa Cruz, fired 54 graduate student employees from their teaching positions on February 28, in the latest escalation of a months-long dispute over student pay, the Santa Cruz Sentinel reports. [The Scientist] Editorial

NEW International Society for Stem Cell Research (ISSCR)
June 24-27, 2020
Boston, Massachusetts, United States

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Fellow – Disuse Atrophy (University of Illinois)

Postdoctoral Researcher – Soft Tissue Biology (Mayo Clinic)

Postdoctoral Fellow – Neuromuscular Disease (Johns Hopkins Medicine)

Postdoctoral Fellow – Vascular Biology (Augusta University)

Postdoctoral Position – Vascular Smooth Muscle and Endothelial Cell Ion Channels (University of Tennessee Health Science Center)

Academic Clinical Lecturer – Clinical Pharmacology and Therapeutics Cardiology (University of Bristol)

Postdoctoral Position – Molecular Skeletal Muscle Cell Signaling (University of Copenhagen)

Postdoctoral Fellow – Cardiac Tissue Engineering and Regeneration (Duke University)

Postdoctoral Research Associate – Muscle Stem Cell Biology (State University of New York)

Assistant Specialist – Cardiovascular Medicine (University of California, Davis)

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