Muscle Cell News Volume 5.17 | May 11 2020

TOP STORY

A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells

Researchers profiled the heterogeneous cell cultures generated from multiple human pluripotent stem cell (hPSC) myogenic differentiation protocols and mapped hPSC-derived myogenic progenitors to an embryonic-to-fetal transition period. [Cell Stem Cell] Abstract | Graphical Abstract

(Ranked by impact factor of the journal)

CARDIAC MUSCLE CELLS

The Calcineurin-TFEB-p62 Pathway Mediates the Activation of Cardiac Macroautophagy by Proteasomal Malfunction

In cultured cardiomyocytes, systemic proteasome inhibition-induced increased in transcription factor EB (TFEB) activation and p62 expression were blunted by pharmacological and genetic calcineurin inhibition and by siRNA-mediated Molcn1 silencing. [Circ Res] Abstract

Multiparametric Mechanistic Profiling of Inotropic Drugs in Adult Human Primary Cardiomyocytes

The authors developed an adult human primary cardiomyocyte contractility model that had the potential to provide a predictive preclinical approach for simultaneously predicting drug-induced inotropic effect. [Sci Rep] Full Article

Formation of an Electrical Coupling Between Differentiating Cardiomyocytes

Syncytium forming human induced pluripotent stem cell-derived cardiomyocytes were harvested and seeded on a new substrate on different days of differentiation. The excitation conduction in a sample was characterized by the stability of the wavefront using optical mapping data. [Sci Rep] Full Article

Functional Culture and In Vitro Genetic and Small-Molecule Manipulation of Adult Mouse Cardiomyocytes

Scientists established murine primary adult cardiomyocyte culture methods that enhanced survival and maintained sarcomeric structure and Ca²⁺ cycling to enable physiologically relevant contractile force measurements. [Commun Biol] Full Article

SKELETAL MUSCLE CELLS

Adipogenesis of Skeletal Muscle Fibro/Adipogenic Progenitors Is Affected by the WNT5a/GSK3/β-Catenin Axis

GSK3 inhibition improved the fibro/adipogenic progenitor pro-myogenic role by efficiently stimulating, via follistatin secretion, muscle satellite cell differentiation into mature myotubes. [Cell Death Differ] Abstract

MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway

In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. [Int J Mol Sci] Full Article

MicroRNA-127-3p Regulates Myoblast Proliferation by Targeting Sept7

Overexpression of miR-127-3p significantly inhibited proliferation of C2C12 cells and vice versa. Sept7 was a target gene of miR-127-3p using dual-luciferase reporter assay, qRT-PCR, and Western blotting. [Biotechnol Lett] Abstract

SMOOTH MUSCLE CELLS

Targeting Mitochondrial Fission as a Potential Therapeutic for Abdominal Aortic Aneurysm

In abdominal aortic vascular smooth muscle cells, angiotensin II induced phosphorylation of dynamin-related protein 1 (Drp1) and mitochondrial fission, the latter of which was attenuated with Drp1 silencing as well as mdivi1. [Cardiovasc Res] Abstract | Graphical Abstract

NLRC3 Inhibits PDGF-Induced PASMCs Proliferation via PI3K-mTOR Pathway

Researchers found that the proliferation of pulmonary artery smooth muscle cells (PASMCs) stimulated with platelet-derived growth factor (PDGF) decreased when phosphoinositide 3-kinase (PI3K) or mammalian target of rapamycin (mTOR) inhibitors pretreatment. [J Cell Physiol] Abstract

MicroRNA-29a-3p Regulates Abdominal Aortic Aneurysm Development and Progression via Direct Interaction with PTEN

Quantitative real-time polymerase chain reaction examined miRNA-19a-3p and PMEPA1 expression in multiplied vascular smooth muscle cells. Cell transfection upregulated or downregulated the genes and cell counting kit-8 assay determined cellular viability. [J Cell Physiol] Abstract

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Cardiomyocyte Ageing and Cardioprotection: Consensus Document from the ESC Working Groups Cell Biology of the Heart and Myocardial Function

The authors review the current knowledge about the pathophysiology of cardiac aging that eventually impacts on the increased susceptibility of cells to ischemia/reperfusion injury and can affect the efficiency of cardioprotective strategies. [Cardiovasc Res] Abstract

Visit our reviews page to see a complete list of reviews in the muscle cell research field.

Santhera Signs Agreements in Gene Therapy Research for Congenital Muscular Dystrophy with Rutgers University

Santhera Pharmaceuticals announces the signing of two agreements with Rutgers, The State University of New Jersey as part of its program to advance gene therapy research for the treatment of LAMA2-deficient congenital muscular dystrophy. [Santhera Pharmaceuticals] Press Release

FDA Grants Fast Track Designation For Omecamtiv Mecarbil In Heart Failure

Amgen and Cytokinetics, Incorporated announced that the FDA has granted Fast Track designation for omecamtiv mecarbil, a novel selective cardiac myosin activator, being developed for the potential treatment of chronic heart failure with reduced ejection fraction. [Amgen] Press Release

Secretive Jasons to Offer Advice on How to Reopen Academic Labs Shut by Pandemic

A group of prominent academic scientists that has been advising the US government on security matters since the Cold War is conducting a quick-turnaround, pro bono study of a new threat to national security-the impact of COVID-19 on academic research. And this time it’s personal. [ScienceInsider] Editorial

How Swamped Preprint Servers Are Blocking Bad Coronavirus Research

Repositories are rapidly disseminating crucial pandemic science — and they’re screening more closely to guard against poor-quality work. [Nature News] Editorial

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NEW Postdoctoral Position – Cell Biology of Cardiovascular Diseases (University Medical Center Göttingen)

Postdoctoral Fellow – Musculoskeletal Regenerative Medicine (Novartis)

Research Associate – In Vitro Pharmacology in Skeletal Muscle (Evotec International GmbH)

Postdoctoral Position – Muscle Denervation and Axonal Degeneration Research (Flanders Institute for Biotechnology)

Postdoctoral Research Fellow – Therapy for Cardiovascular Disease (University of Iowa)

Postdoctoral Research Associate – Molecular Mechanisms in Heart Disease (The University of Arizona College of Medicine)

Postdoctoral Fellow – NAD Metabolism in Heart Disease (Oklahoma Medical Research Foundation)

Associate Professor – Cancer Biology (University of Cincinnati)

Group Leader – Cancer Biology (Institute of Pharmacology and Structural Biology)

Postdoctoral Research Associate – Muscle Stem Cell Biology (State University of New York)

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