Muscle Cell News Volume 6.26 | Jul 26 2021

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    2021-07-26 | MCN 6.26


    Muscle Cell News by STEMCELL Technologies
    Vol. 6.26 – 26 July, 2021
    TOP STORY

    Dynamic Loading of Human Engineered Heart Tissue Enhances Contractile Function and Drives a Desmosome-Linked Disease Phenotype

    Researchers developed a new dynamic engineered heart tissue model that enabled them to tune preload and have unconstrained contractile shortening of >10%. For disease modeling, they used human induced pluripotent stem cell-derived cardiomyocytes from a patient with arrhythmogenic cardiomyopathy due to mutations in the desmoplakin gene.
    [Science Translational Medicine]

    AbstractPress Release

    How to prioritize and manage your research projects
    PUBLICATIONSRanked by the impact factor of the journal

    Massively Parallel In Vivo CRISPR Screening Identifies RNF20/40 as Epigenetic Regulators of Cardiomyocyte Maturation

    The authors used in vivo somatic Cas9 mutagenesis to perform an in vivo forward genetic screen in mice to identify regulators of cardiomyocyte (CM) maturation, the coordinated changes in phenotype and gene expression that occur in neonatal CMs.
    [Nature Communications]

    Full Article

    Gain-of-Function Mutation in Ubiquitin-Ligase KLHL24 Causes Desmin Degradation and Dilatation in hiPSC-Derived Engineered Heart Tissues

    Dynamically loaded engineered heart tissues (dyn-EHTs) were generated from human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes from two patients and three (non)familial controls. KLHL24 RNA interference or direct desmin overexpression recovered desmin protein levels, restoring morphology and function in patient-derived dyn-EHTs.
    [Journal of Clinical Investigation]

    AbstractFull ArticleGraphical Abstract

    Expression Dynamics of HAND1/2 in In Vitro Human Cardiomyocyte Differentiation

    Scientists established two triple-reporter human induced pluripotent stem cell lines that expressed HAND1mCherry, HAND2EGFP and either MYH6-driven iRFP670 or tagBFP constitutively and investigated their expression dynamics during cardiac differentiation.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    The Bromodomains of the Mammalian SWI/SNF (mSWI/SNF) ATPases Brahma (BRM) and Brahma Related Gene 1 (BRG1) Promote Chromatin Interaction and Are Critical for Skeletal Muscle Differentiation

    Pharmacological inhibition of mSWI/SNF bromodomain function using the small molecule PFI-3 reduced differentiation in cell culture and in vivo through decreased myogenic gene expression, while increasing cell cycle-related gene expression and the number of cells remaining in the cell cycle.
    [Nucleic Acids Research]

    Full ArticleGraphical Abstract

    Human Dystrophin Expressing Chimeric (DEC) Cell Therapy Ameliorates Cardiac, Respiratory, and Skeletal Muscle’s Function in Duchenne Muscular Dystrophy

    Investigators demonstrated that systemic-intraosseous transplantation of DEC human cells derived from myoblasts of normal and Duchenne muscular dystrophy (DMD)-affected donors, increased dystrophin expression in cardiac, respiratory, and skeletal muscles of the mdx/scid mouse model of DMD.
    [Stem Cells Translational Medicine]

    Full Article

    FGGY Carbohydrate Kinase Domain Containing Is Expressed and Alternatively Spliced in Skeletal Muscle and Attenuates MAP Kinase and Akt Signaling

    To better characterize the molecular genetic events of atrophy, a microarray analysis revealed that FGGY carbohydrate kinase domain containing was expressed in skeletal muscle and was induced in response to denervation.
    [Gene]

    Abstract

    IRE1α Regulates Skeletal Muscle Regeneration through Myostatin mRNA Decay

    Researchers found in mice that inositol-requiring enzyme 1 α (IRE1α) was activated during injury-induced muscle regeneration, and muscle-specific IRE1α ablation resulted in impaired regeneration upon cardiotoxin-induced injury.
    [Journal of Clinical Investigation]

    AbstractGraphical Abstract

    CIAPIN1 Accelerates Vascular Remodelling via p53 and JAK2-STAT3 Regulation in Vascular Smooth Muscle Cells

    The authors used loss/gain-of-function approaches by gene delivery to determine whether cytokine-induced apoptosis inhibitor 1 (CIAPIN1) modulated vascular smooth muscle cell proliferation, migration, and neointima formation, and to define the underlying mechanisms.
    [British Journal of Pharmacology]

    Abstract

    Smooth Muscle Cell–Enriched Long Noncoding RNA Regulates Cell Plasticity and Atherosclerosis by Interacting with Serum Response Factor

    Scientists identified a vascular smooth muscle cell (VSMC)-enriched long noncoding RNA cardiac mesoderm enhancer-associated noncoding RNA (CARMN) that was dynamically regulated with progression of atherosclerosis. In both mouse and human atherosclerotic plaques, CARMN colocalized with VSMCs and was expressed in the nucleus.
    [Arteriosclerosis Thrombosis and Vascular Biology]

    Abstract

    Diacylglycerol Kinase Inhibition Reduces Airway Contraction by Negative Feedback Regulation of GQ-Signaling

    Activity of diacylglycerol kinase (DGK) isoforms was inhibited in human airway smooth muscle cells by siRNA-mediated knockdown of DGKα and ζ, while pharmacological inhibition was achieved by pan DGK inhibitor I.
    [American Journal of Respiratory Cell and Molecular Biology]

    Abstract

    Dynamic Flow Priming Programs Allow Tuning Up the Cell Layers Properties for Engineered Vascular Graft

    Investigators proposed a novel method to create three-layered tissue-engineered vascular grafts on biocompatible glass fiber scaffolds starting from flat sheet state into tubular shape and to train the resulting tissue using their developed bioreactor system.
    [Scientific Reports]

    Full Article

    Derive and expand human skeletal muscle progenitors in a serum-free setting using MyoCultâ„¢-SF Expansion Supplement Kit (Human).
    REVIEWS

    Ferroptosis: A Cell Death Connecting Oxidative Stress, Inflammation and Cardiovascular Diseases

    The authors provide an overview of the features, process, function, and mechanisms of ferroptosis, and its increasingly connected relevance to oxidative stress, inflammation, and cardiovascular diseases.
    [Cell Death Discovery]

    Full Article

    Update on the Effects of Microgravity on the Musculoskeletal System

    Scientists review the catabolic effects microgravity exposure has on both bone and skeletal muscle cells, as well as their respective progenitor stem cells.
    [NPJ Microgravity]

    Full Article

    INDUSTRY AND POLICY NEWS

    VERQUVO® (Vericiguat) Approved in the European Union

    Merck announced that the European Commission has granted marketing authorization in the European Union for soluble guanylate cyclase stimulator VERQUVO®, indicated for the treatment of symptomatic chronic heart failure in adult patients with reduced ejection fraction.
    [Merck]

    Full Article

    FEATURED EVENT

    3D Cell Culture

    December 2, 2021
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    JOB OPPORTUNITIES

    Manager – Organoid Core Facility

    Weill Cornell Medicine – New York, New York, United States

    Postdoctoral Associate – Extracellular Matrix and Inflammation

    Baylor College of Medicine – Houston, Texas, United States

    Postdoctoral Fellow – Skeletal Muscle Aging

    Pompeu Fabra University – Barcelona, Spain

    Postdoctoral Training Fellow – Organ Morphodynamics

    Francis Crick Institute – London, England, United Kingdom

    Postdoctoral Fellow – Modeling of Cardiac Disease

    University of Pittsburgh – Pittsburgh, Pennsylvania, United States

    > See All Jobs

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