In vivo and in vitro data show glucocorticoid receptor (GR) functions in microglial differentiation, proliferation and motility. Interestingly, microglial GR also abolishes the lipopolysaccharide-induced delayed outward rectifier currents by downregulating Kv1.3 expression known to control microglia proliferation and oxygen radical production. [Cell Death Differ] Abstract Minimally Invasive Transplantation of iPSC-Derived ALDHhiSSCloVLA4+ Neural Stem Cells Effectively Improves the Phenotype of an Amyotrophic Lateral Sclerosis Model
Researchers isolated a specific neural stem cell (NSC) population from human induced pluripotent stem cells (iPSCs) based on high aldehyde dehydrogenase activity, low side scatter, and integrin VLA4 positivity. They assessed the therapeutic effects of these NSCs on the phenotype of amyotrophic lateral sclerosis mice after intrathecal or intravenous injections. [Hum Mol Genet] Abstract
C1q-Tumor Necrosis Factor-Related Protein 8 (CTRP8) Is a Novel Interaction Partner of Relaxin Receptor RXFP1 in Human Brain Cancer Cells
Researchers report a novel ligand-receptor system composed of the leucine-rich G protein coupled relaxin receptor, RXFP1, and the CTRP8 in human primary brain cancer, a tumor entity devoid of the classical RXFP1 ligands, RLN1-3. [J Pathol] Abstract
Development of a Culture System to Induce Microglia-Like Cells from Hematopoietic Cells
Researchers established a culture system to induce microglia-like cells from hematopoietic cells by co-culture with astrocytes. [Neuropathol Appl Neurobiol] Abstract | Full Article
Stepwise, Non-Adherent Differentiation of Human Pluripotent Stem Cells to Generate Basal Forebrain Cholinergic Neurons via Hedgehog Signaling
Scientists demonstrated the efficient production of basal forebrain cholinergic neurons, using a novel embryoid body based non-adherent differentiation protocol. They established a specific basal forebrain neural stem cell phenotype via expression of the basal forebrain transcription factors NKX2.1 and LHX8, as well as the general forebrain marker FOXG1. [Stem Cell Res] Abstract
Collagen Microsphere Serving as a Cell Carrier Supports Oligodendrocyte Progenitor Cell Growth and Differentiation for Neurite Myelination In Vitro
Investigators showed that the viability of oligodendrocyte progenitor cells, B104 cells and PC12 cells grown on microspheres was not significantly different with those in cell culture plates. [Stem Cell Res Ther]
Abstract | Full Article
The Influence of Immunosuppressive Drugs on Neural Stem/Progenitor Cell Fate In Vitro
Researchers investigated the effects of different immunotherapeutic strategies on human neural progenitor cell survival/death, proliferation, metabolic activity, differentiation and migration in vitro. [Exp Cell Res] Abstract
Functional Screening Assays with Neurons Generated from Pluripotent Stem Cell-Derived Neural Stem Cells
Researchers developed an efficient protocol to culture pure populations of neurons, as confirmed by gene expression analysis, in the 96-well format necessary for screens. [J Biomol Screen] Abstract
Selenium Compounds Prevent Amyloid β-Peptide Neurotoxicity in Rat Primary Hippocampal Neurons
Researchers identified the neuroprotective effects of the selenium compounds on the neurotoxicity of amyloid β(1-42) in primary cultures of murine hippocampal neurons. [Neurochem Res] Abstract
Activation of Developmental Nuclear Fibroblast Growth Factor Receptor 1 Signaling and Neurogenesis in Adult Brain by α7 Nicotinic Receptor Agonist
Scientists report that targeting the α7 nicotinic acetylcholine receptors with a specific TC-7020 agonist led to a robust accumulation of endogenous fibroblast growth factor receptor 1 (FGFR1) in the cell nucleus. Nuclear FGFR1 accumulation was accompanied by an inhibition of proliferation of neural stem/progenitor cells in the subventricular zone and by the generation of new neurons. [Stem Cells Transl Med] Abstract