Real-Time Discrimination between Proliferation and Neuronal and Astroglial Differentiation of Human Neural Stem Cells Researchers report a real-time and label-free method that uses a capacitance sensor array to monitor the differentiation of human fetal brain-derived neural stem cells and to identify the fates of differentiated cells. [Sci Rep] Full Article Neurogenin 2 Mediates Amyloid-β Precursor Protein-Stimulated Neurogenesis Neurogenin 2 (Ngn2) overexpression in amyloid-β precursor protein knockout mice neural stem/progenitor cells (NSPCs) promoted neuronal differentiation, whereas siRNA knockdown of Ngn2 expression in wild-type NSPCs decreased neuronal differentiation. [J Biol Chem] Abstract | Full Article MACF1 Regulates the Migration of Pyramidal Neurons via Microtubule Dynamics and GSK-3 Signaling Scientists show that microtubule actin crosslinking factor 1 (MACF1) determines neuronal positioning by regulating microtubule dynamics and mediating GSK-3 signaling during brain development. [Dev Biol] Abstract Triple-Layered Mixed Co-Culture Model of RPE Cells with Neuroretina for Evaluating the Neuroprotective Effects of Adipose-MSCs Researchers aimed to establish a viable mixed co-culture model having three layers: neuroretina explants, retinal pigment epithelium (RPE) cells and adipose tissue-derived mesenchymal stem cells (MSCs) for evaluating adipose-MSC effects. [Cell Tissue Res] Abstract Communication between Mast Cells and Rat Submucosal Neurons Histamine evoked a biphasic rise of the cytosolic Ca2+ concentration in cultured submucosal neurons, consisting in a release of intracellularly stored Ca2+ followed by an influx from the extracellular space. [Pflugers Arch] Abstract Deficiency of Trim27 Protects Dopaminergic Neurons from Apoptosis in the Neurotoxin Model of Parkinson’s Disease Researchers showed that Trim27 expression was dramatically induced in PC12 cells and in the substantia nigra pars compacta of the Parkinson’s disease mouse model. RNAi-mediated knockdown of Trim27 in PC12 cells showed obvious suppression of apoptosis. [Brain Res] Abstract DNA Topoisomerase IIβ as a Molecular Switch in Neural Differentiation of Mesenchymal Stem Cells Scientists evaluated the subset of genes that are regulated in neural transdifferentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) in vitro and found genes related with DNA topoisomerase IIβ (topo IIβ). For this purpose, topo IIβ was silenced by specific small interfering RNAs in hMSCs and cells were induced to differentiate into neuron-like cells. [Ann Hematol] Abstract Human Primary Mixed Brain Cultures: Preparation, Long-Term Maintenance, Characterization and Application to Neuroscience Research Scientists describe preparation, maintenance and major characteristics of a primary human mixed brain culture, including neurons, obtained from legally aborted fetal brain tissue. This approach employs standard materials and techniques used in the preparation of rodent neuron cultures, with critical modifications. [Mol Brain] Abstract | Full Article Human iPSC Neurons Display Activity-Dependent Neurotransmitter Secretion: Aberrant Catecholamine Levels in Schizophrenia Neurons Scientists investigated human-induced pluripotent stem cell (hiPSC)-derived neurons for their ability to secrete neurotransmitters in an activity-dependent manner, the fundamental property required for chemical neurotransmission. [Stem Cell Rep] Full Article | Graphical Abstract | Press Release Chemical Conversion of Human Fibroblasts into Functional Schwann Cells The authors present a gene-free approach that allows efficient conversion of human fibroblasts via a transient progenitor stage into Schwann cells, the major glial cell type of peripheral nerves. [Stem Cell Rep] Full Article | Graphical Abstract |