Elucidating Molecular Phenotypes Caused by the SORL1 Alzheimer’s Disease Genetic Risk Factor Using Human Induced Pluripotent Stem Cells Scientists used hIPSCs to examine genetic variation in the SORL1 gene and possible contributions to sporadic Alzheimer’s disease (SAD)-related phenotypes in human neurons. They found that human neurons carrying SORL1 variants associated with an increased SAD risk show a reduced response to treatment with BDNF, at the level of both SORL1 expression and APP processing. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Photosensitivity of Neurons Enabled by Cell-Targeted Gold Nanoparticles Researchers showed that gold nanoparticles can be conjugated to high-avidity ligands for a variety of cellular targets. Once bound to a neuron, these particles transduce millisecond pulses of light into heat, which changes membrane capacitance, depolarizing the cell and eliciting action potentials. [Neuron] Abstract Adult Hippocampal Neural Stem and Progenitor Cells Regulate the Neurogenic Niche by Secreting VEGF Scientists showed that adult hippocampal neural stem and progenitor cells (NSPCs) secrete surprisingly large quantities of the essential growth factor VEGF in vitro and in vivo. This self-derived VEGF is functionally relevant for maintaining the neurogenic niche as inducible, NSPC-specific loss of VEGF results in impaired stem cell maintenance despite the presence of VEGF produced from other niche cell types. [Proc Natl Acad Sci USA] Abstract PKCη/Rdx-Driven Phosphorylation of PDK1: A Novel Mechanism Promoting Cancer Cell Survival and Permissiveness for Parvovirus-Induced Lysis Scientists revealed a new protoparvovirus-induced intracellular loop-back mechanism whereby PKCη/Rdx phosphorylates mouse PDK1:S138 and activates it independently of PI3-kinase signaling. The corresponding human PDK1phosphoS135 appears as a hallmark of highly aggressive brain tumors and may contribute to the very effective targeting of human gliomas by H-1PV. [PLoS Genet] Full Article | Press Release Mouse Low-Grade Gliomas Contain Cancer Stem Cells with Unique Molecular and Functional Properties Researchers reported the isolation of CD133+ multi-potent low-grade glioma stem cells, which generate glioma-like lesions histologically similar to the parent tumor following injection into immunocompetent hosts. [Cell Rep] Full Article | Graphical Abstract | Press Release Differentiation of Human Induced Pluripotent Stem Cells to Mature Functional Purkinje Neurons Scientists derived induced pluripotent stem cells (iPSCs) from human fibroblasts and directed the specification of iPSCs first to Purkinje progenitors, by adding Fgf2 and insulin to the embryoid bodies (EBs) in a time-sensitive manner, which activates the endogenous production of Wnt1 and Fgf8 from EBs that further patterned the cells towards a midbrain-hindbrain-boundary tissue identity. [Sci Rep] Full Article Suppression of Adenosine 2a Receptor (A2aR)-Mediated Adenosine Signaling Improves Disease Phenotypes in a Mouse Model of Amyotrophic Lateral Sclerosis Researchers showed that A2aR, but not adenosine 1 receptor, is highly enriched in spinal (motor) neurons. A2aR expression is also selectively increased at the symptomatic onset in the spinal cords of SOD1G93A mice and end-stage human amyotrophic lateral sclerosis spinal cords. [Exp Neurol] Abstract V-Myc Immortalizes Human Neural Stem Cells in the Absence of Pluripotency-Associated Traits Scientists investigated the expression of surface, biochemical and genetic markers of stemness and pluripotency in v-myc immortalized and control human neural stem cells and compared these two cell types to human embryonic stem cells and fibroblasts. [PLoS One] Full Article Paraoxon and Pyridostigmine Interfere with Neural Stem Cell Differentiation Researchers hypothesized that rat neural progenitor cells extracted on embryonic day 14.5 would be affected by constant inhibition of acetylcholinesterase from chronic exposure to organophosphate or pyridostigmine during differentiation. [Neurochem Res] Abstract Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain Scientists obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes. [Int J Mol Sci] Abstract | Download Full Article |