Human Neuropsychiatric Disease Modeling Using Conditional Deletion Reveals Synaptic Transmission Defects Caused by Heterozygous Mutations in NRXN1 The authors generated human ESCs with different heterozygous conditional NRXN1 mutations and analyzed two different types of isogenic control and NRXN1 mutant neurons derived from these ESCs. Both heterozygous NRXN1 mutations selectively impaired neurotransmitter release in human neurons without changing neuronal differentiation or synapse formation. [Cell Stem Cell] Abstract | Graphical Abstract Upregulation of miR-181s Reverses Mesenchymal Transition by Targeting KPNA4 in Glioblastoma Researchers explored the most effective miRNAs affecting glioblastoma multiforme phenotype transition and malignant progression. They identified the antitumorigenic activity of miR-181b in vitro and in vivo. [Sci Rep] Full Article Effects of ECM Protein Micropatterns on the Migration and Differentiation of Adult Neural Stem Cells Investigators applied soft-lithographic technique to design simple and reproducible laminin-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. [Sci Rep] Full Article Effect of the JAK2/STAT3 Inhibitor SAR317461 on Human Glioblastoma Tumorspheres Researchers report that the JAK2 inhibitor SAR317461 clearly inhibited STAT3 phosphorylation and had substantial activity against cells from six of seven different patient GSC derived glioblastoma multiforme (GBM) tumorsphere lines and three immortalized GBM lines. [J Transl Med] Full Article Comparative Analysis of Human and Rodent Brain Primary Neuronal Culture Spontaneous Activity Using Micro-Electrode Array Technology A direct comparison of spontaneous spiking activity between rodent and human primary neurons grown under the same in vitro conditions using micro electrode array technology has never been carried out before and is described. [J Cell Biochem] Abstract Dexamethasone-Mediated Activation of Fibronectin Matrix Assembly Reduces Dispersal of Primary Human Glioblastoma Cells Scientists utilized a broad range of qualitative and quantitative assays and, a human Glioblastoma multiforme (GBM) tissue microarray, and freshly-isolated primary human GBM cells grown both as conventional 2D cultures and as 3D spheroids to explore the role of Dex and fibronectin matrix assembly in modulating various parameters that can significantly influence tumor cell dispersal. [PLoS One] Full Article A Perturbed MicroRNA Expression Pattern Characterizes Embryonic Neural Stem Cells Derived from a Severe Mouse Model of Spinal Muscular Atrophy (SMA) Scientists characterized for the first time neural stem cells (NSCs) derived from embryonic spinal cords of a severe survival motor neuron (SMN)Δ7M SMA mouse model. SMNΔ7 NSCs behave as their wild type counterparts, when neurosphere formation ability and the expression levels of specific regional and self-renewal markers is considered. [Int J Mol Sci] Abstract | Download Full Article | Press Release The Expression of CUGBP1 after Spinal Cord Injury in Rats The authors performed acute spinal cord injury (SCI) modelling in adult rats in order to research the expression changes of CUG-binding protein 1 (CUGBP1) in spinal cord. They also examined the expression profiles of p27, which was up-regulated after SCI. To further understand whether CUGBP1 plays a role in astrocyte proliferation, they applied LPS to induce astrocyte proliferation in vitro. [Neurochem Res] Abstract Involvement of Inhibitory PAS Domain Protein in Neuronal Cell Death in Parkinson’s Disease Scientists showed that inhibitory PAS domain protein (IPAS) is a key molecule involved in neuronal cell death in Parkinson’s disease. IPAS was ubiquitinated by Parkin for proteasomal degradation following carbonyl cyanide m-chlorophenyl hydrazone treatment. [Cell Death Discov] Full Article |