Organoid News Volume 2.05 | Feb 11 2021

May 12, 2022

ON 2.05 | Feb 11 2021

Vol. 2.05 – 11 February, 2021

TOP STORY

Human Heart-Forming Organoids Recapitulate Early Heart and Foregut Development

Scientists generated complex, highly structured, 3D heart-forming organoids to study genetic defects in vitro.
[Nature Biotechnology]

Abstract

Click to learn more about the STEMdiff Cerebral Organoid Kit from STEMCELL Technologies

PUBLICATIONSRanked by the impact factor of the journal

Bio‐Assembling
Macro‐Scale, Lumenized Airway Tubes of Defined Shape via Multi‐Organoid Patterning and Fusion

A robust organoid engineering approach, Multi‐Organoid Patterning and Fusion was presented to assemble individual airway organoids of different sizes into upscaled, scaffold‐free airway tubes with predefined shapes.
[Advanced Science]

Full Article Press Release

Distinct
Mechanisms for TMPRSS2 Expression Explain Organ-Specific Inhibition of SARS-CoV-2 Infection by Enzalutamide

Investigators evaluated the antiviral efficacy of enzalutamide in prostate cancer cells, lung cancer cells, human lung organoids and Ad-ACE2-transduced mice.
[Nature Communications]

Full Article

SPT6
Promotes Epidermal Differentiation and Blockade of an Intestinal-Like Phenotype through Control of Transcriptional Elongation

Researchers showed that epidermal genes, including ~30% of induced differentiation genes already contain stalled Pol II at the promoters in epidermal stem and progenitor cells which was then released into productive transcription elongation upon differentiation.
[Nature Communications]

Full Article

Epithelial Response to IFN-γ Promotes SARS-CoV-2 Infection

Using primary epithelial organoids from human colon, the authors explored how the central antiviral mediator IFN‐γ, which is elevated in COVID‐19, affected epithelial cell differentiation, ACE2 expression, and susceptibility to infection with SARS‐CoV‐2.
[EMBO Molecular Medicine]

Abstract Full Article

Inhibition
of DHFR Targets the Self-Renewing Potential of Brain Tumor Initiating Cells

Scientists focused on dihydrofolate reductase, a key enzyme in one-carbon metabolism, and demonstrated this enzyme’s overexpression in several human brain tumors and its expression in human brain tumor initiating cells.
[Cancer Letters]

Full Article

Electromembrane
Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

Investigators demonstrated that electromembrane extraction based on electrophoresis across an oil membrane was suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry-based measurements.
[Analytical Chemistry]

Full Article
Graphical Abstract

Hsp90-Stabilized
MIF Supports Tumor Progression via Macrophage Recruitment and Angiogenesis in Colorectal Cancer

The authors showed that migration inhibitory factor stabilization was tumor-specific in an acute colitis-associated colorectal cancer mouse model, leading to tumor-specific functions and selective therapeutic vulnerabilities.
[Cell Death & Disease]

Full Article

Lipidome-Based
Targeting of STAT3-Driven Breast Cancer Cells Using Poly-L-Glutamic Acid-Coated Layer-by-Layer Nanoparticles

Scientists found that poly-L-glutamic acid (PLE)-coated nanoparticles (NPs) bond to STAT3-transformed breast cancer cells with 50% greater efficiency than to non-transformed cells, and the heightened PLE-NP binding to triple-negative breast cancer cells was attenuated by STAT3 inhibition.
[Molecular Cancer Therapeutics]

Abstract

Gel-Free
3D Tumoroids with Stem Cell Properties Modeling Drug Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer

Researchers investigated whether cisplatin, imatinib, and 5-fluorouracil altered the tumoroid growth of metastatic colorectal cancer.
[Cells]

Full Article

Immunofluorescence image of adult stem cell-derived intestinal organoids.

REVIEWS

Emerging
Biofabrication Approaches for Gastrointestinal Organoids towards Patient Specific Cancer Models

Scientists outline the main challenges and possible risks regarding the potential translation of gastrointestinal organoids from laboratory settings to patient-specific models in clinical applications.
[Cancer Letters]

Abstract

Advances
in Modeling the Human Microbiome–Gut–Brain Axis In Vitro

The discovery of stem cells has offered a new source of cells, while their use in generating gastrointestinal and brain organoids has enabled the development of novel 3D tissues that better mimic the native tissue structure and function. Researchers discuss how recent advances and trends in this area can be applied in host–pathogen interaction studies.
[Biochemical Society Transactions]

Full Article

The
Emerging Role of Neuronal Organoid Models in Drug Discovery: Potential Applications and Hurdles to Implementation

The authors provide an overview of the gaps to broader implementation of neuronal organoids in a drug discovery setting, as well as emerging technologies that may better enable their utilization.
[Molecular Pharmacology]

Abstract

INDUSTRY AND POLICY NEWS

Leading Innovators Collaborate to Provide New, Patient Representative Cell Culture Solutions for Smarter In Vitro Research

One of the biggest challenges in drug development and personalized medicine is how to model the cellular microenvironment accurately. Cellaria and BioLamina are pursuing this challenge by collaborating to develop more defined reprogramming methods, large-scale iPSC expansions, and lineage differentiation for human cells and animal cells for vertebrate and non-vertebrate species.
[Cellaria, Inc. and BioLamina (Businesswire, Inc.)]

Press Release

FEATURED EVENT

Human Heart-Forming Organoids Recapitulate Early Heart and Foregut Development

Bio‐Assembling
Macro‐Scale, Lumenized Airway Tubes of Defined Shape via Multi‐Organoid Patterning and Fusion

Distinct
Mechanisms for TMPRSS2 Expression Explain Organ-Specific Inhibition of SARS-CoV-2 Infection by Enzalutamide

SPT6
Promotes Epidermal Differentiation and Blockade of an Intestinal-Like Phenotype through Control of Transcriptional Elongation

Epithelial Response to IFN-γ Promotes SARS-CoV-2 Infection

Inhibition
of DHFR Targets the Self-Renewing Potential of Brain Tumor Initiating Cells

Electromembrane
Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

Hsp90-Stabilized
MIF Supports Tumor Progression via Macrophage Recruitment and Angiogenesis in Colorectal Cancer

Lipidome-Based
Targeting of STAT3-Driven Breast Cancer Cells Using Poly-L-Glutamic Acid-Coated Layer-by-Layer Nanoparticles

Gel-Free
3D Tumoroids with Stem Cell Properties Modeling Drug Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer

Emerging
Biofabrication Approaches for Gastrointestinal Organoids towards Patient Specific Cancer Models

Advances
in Modeling the Human Microbiome–Gut–Brain Axis In Vitro

The
Emerging Role of Neuronal Organoid Models in Drug Discovery: Potential Applications and Hurdles to Implementation

Leading Innovators Collaborate to Provide New, Patient Representative Cell Culture Solutions for Smarter In Vitro Research

ACCR: Radiation Science and Medicine

> See All Events

Postdoctoral Researcher – 3D In Vitro Systems for Respiratory Bacterial Infections

Postdoctoral Fellow – Neural Organ-on-Chips

Faculty Position – Department of Hematology

Postdoctoral
Researcher – Neuroimmunology

Research
Technician – Stem Cell and Genome Editing

> See All Jobs