Organoid News Volume 3.08 | Mar 3 2022

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    Justin.choi
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    2022-03-03 | ON 3.07


    Organoid News by STEMCELL Technologies
    Vol. 3.07 – 3 March, 2022
    TOP STORY

    Modeling Injury and Repair in Kidney Organoids Reveals That Homologous Recombination Governs Tubular Intrinsic Repair

    Single-nuclear RNA sequencing of kidney organoids after cisplatin exposure identified 159 differentially expressed genes and 29 signal pathways in tubular cells undergoing intrinsic repair.
    [Science Translational Medicine]

    Abstract
    Explore neural organoid technologies with this free wallchart from STEMCELL and Nature Neuroscience
    PUBLICATIONSRanked by the impact factor of the journal

    3D Microgels to Quantify Tumor Cell Properties and Therapy Response Dynamics

    A 3D in vitro tumor model using micro-molded hydrogels, the Gels for Live Analysis of Compartmentalized Environments platform, was adapted into a 96-well plate format that integrated patient-derived organoids and was combined with longitudinal automated imaging.
    [Biomaterials]

    Abstract

    DGAT2 Inhibition Potentiates Lipid Droplet Formation to Reduce Cytotoxicity in APOL1 Kidney Risk Variants

    Scientists used CRISPR-Cas9 gene editing of iPSCs to develop human derived APOL1G0/G0 and apolipoprotein L1 (APOL1)G2/G2M kidney organoids on an isogenic background, and performed bulk RNA sequencing of organoids before and after treatment with IFN-γ.
    [Journal of the American Society of Nephrology]

    Abstract

    An HNF1α Truncation Associated with Maturity-Onset Diabetes of the Young Impairs Pancreatic Progenitor Differentiation by Antagonizing HNF1β Function

    Investigators used maturity-onset diabetes of a young 3 patient and CRISPR/Cas9-engineered human hiPSCs grown as 3D organoids to investigate how HNF1αp291fsinsC affected hiPSC differentiation during pancreatic development.
    [Cell Reports]

    Full ArticleGraphical AbstractPress Release

    The Fibrillin-1/VEGFR2/STAT2 Signaling Axis Promotes Chemoresistance via Modulating Glycolysis and Angiogenesis in Ovarian Cancer Organoids and Cells

    RNA sequencing of cisplatin-resistant and -sensitive ovarian cancer organoids was performed, followed by detection of the expression level of fibrillin-1 in organoids and clinical specimens of ovarian cancer.
    [Cancer Communications]

    Full Article

    Single-Cell Sequencing Reveals PDFGRα+ Stromal Cell Subpopulations That Promote Proacinar Differentiation in Embryonic Salivary Gland Organoids.

    Investigators performed scRNA-seq and identified multiple stromal cell subsets, including Pdgfrα+ stromal subsets expressing both Fgf2 and Fgf10.
    [Development]

    Abstract

    Modelling T Cell Immunity against Hepatitis C Virus with Liver Organoids in a Microfluidic Coculture System

    Researchers developed a CD8+ T cell and adult stem cell liver organoid system using a microfluidic chip to coculture 3D human liver organoids embedded in extracellular matrix with HLA-matched primary human T cells in suspension.
    [Open Biology]

    Full Article

    Small Molecule LATS Kinase Inhibitors Block the Hippo Signaling Pathway and Promote Cell Growth under 3D Culture Conditions

    The authors found that a molecule designated GA-017 increased the number and size of spheroids of various cell-types in both scaffold-based and scaffold-independent cultures.
    [Journal of Biological Chemistry]

    Full Article

    Short-Term Intermittent Cigarette Smoke Exposure Enhances Alveolar Type 2 Cell Stemness via Fatty Acid Oxidation

    Scientists exposed mice to three weeks of continuous or intermittent cigarette smoke (CS) to identify whether different CS exposure patterns would result in differential effects on stem cells and the mechanisms underlying these potential differences.
    [Respiratory Research]

    Full Article

    Watch 'collaborating to accelerate COVID-19 research'
    REVIEWS

    Challenges to, and Prospects for, Reverse Engineering the Gastrointestinal Tract Using Organoids

    The authors discuss the challenges and current advances of the gastrointestinal tract organoid models.
    [Trends in Biotechnology]

    Full Article

    Organoid Technologies for the Study of Intestinal Microbiota–Host Interactions

    Investigators present recent studies on probiotics and postbiotics in the context of novel discovery tools, such as organoids and organoid-based platforms, and nontransformed preclinical models, that can be generated from intestinal stem cells.
    [Trends in Molecular Medicine]

    Abstract

    When Pooled CRISPR Screen Meets Kidney Organoids: Towards Identification of New Genes and Pathways

    Kidney organoids may complement animal models for annotating and predicting the molecular and cellular function of these candidate genes.
    [Kidney International]

    Abstract
    INDUSTRY AND POLICY NEWS

    MDimune Signs Research Agreement with YiPSCELL to Develop iPSC-Based BioDrone® Therapeutics

    MDimune announced a joint research agreement with YiPSCELL to develop BioDrone® Platform therapeutics based on iPSCs.
    [MDimune (EIN Presswire, Inc.)]

    Press Release

    Nine Engineers Awarded $5.5 Million in NSF Early Career Awards

    Nine faculty members in Penn State’s College of Engineering were recognized with National Science Foundation Faculty Early Career Development awards.
    [PennState]

    Press Release
    FEATURED EVENT

    EMBO: Microbial Infections and Human Cancer

    April 6 – 9, 2022
    Heidelberg, Germany

    > See All Events

    JOB OPPORTUNITIES

    Scientist – Organoid Models

    Frederick National Laboratory – Frederick, Maryland, United States

    Research Technician – Placental Development

    Friedrich Miescher Institute for Biomedical Research – Basel, Switzerland

    Postdoctoral Position – Tissue Engineering and Epigenetics

    University of Lausanne – Lausanne, Switzerland

    Postdoctoral Fellow – Breast Cancer Organoids

    Cold Spring Harbor Laboratory – Cold Spring Harbor, New York, United States

    Researcher Specialist – Resistance in Pancreatic and Liver Cancer

    University Of Wisconsin–Madison – Madison, Wisconsin, United States

    > See All Jobs

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