Organoid News Volume 3.43 | Nov 03 2022

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    Noshin Noorjahan
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    2022-11-03 | ON 3.43


    Organoid News by STEMCELL Technologies
    Vol. 3.43 – 3 November, 2022
    TOP STORY

    Adult Human Kidney Organoids Originate from CD24+ Cells and Represent an Advanced Model for Adult Polycystic Kidney Disease

    Scientists showed that kidney tubuloids could be used to model the most common inherited kidney disease, namely autosomal dominant polycystic kidney disease, reconstituting the phenotypic hallmark of this disease with cyst formation.
    [Nature Genetics]

    Abstract
    Register for Organoids as Models of Development and Disease, and Their Impact on Drug Discovery.
    PUBLICATIONSRanked by the impact factor of the journal

    Clinically Relevant Treatment of PDX Models Reveals Patterns of Neuroblastoma Chemoresistance

    Investigators developed a clinically relevant in vivo treatment protocol mimicking the first-line five-chemotherapy treatment regimen of high-risk neuroblastoma (NB) and applied this protocol to mice with MYCN-amplified NB patient-derived xenografts (PDXs).
    [Science Advances]

    Full Article

    Single Cell Transcriptomics Reveals Distinct Transcriptional Responses to Oxycodone and Buprenorphine by iPSC-Derived Brain Organoids from Patients with Opioid Use Disorder

    To understand how opioids modulated brain cell type function at the single-cell level, researchers performed single nucleus RNA-seq using iPSC-derived forebrain organoids from three male opioid use disorder subjects in response to oxycodone, buprenorphine, or vehicle for seven days.
    [Molecular Psychiatry]

    Full Article

    BRCA Mutational Status Shapes the Stromal Microenvironment of Pancreatic Cancer Linking Clusterin Expression in Cancer Associated Fibroblasts with HSF1 Signaling

    Full Article

    Estrogen Regulates Divergent Transcriptional and Epigenetic Cell States in Breast Cancer

    Researchers systematically tracked estrogen response across time at a single-cell level in multiple cell line and organoid models and developed a computational tool that quantified signaling gradients in single-cell datasets.
    [Nucleic Acids Research]

    Full Article

    Neuropilin-1 Mediates SARS-CoV-2 Infection of Astrocytes in Brain Organoids, Inducing Inflammation Leading to Dysfunction and Death of Neurons

    Investigators found that astrocytes were readily infected by SARS-CoV-2, but surprisingly, neuropilin-1, not angiotensin-converting enzyme 2, served as the principal receptor mediating cell entry.
    [mBio]

    Full Article

    Competency of iPSC-Derived Retinas in MHC-Mismatched Transplantation in Non-Human Primates

    Scientists studied the immunological features of iPSC-retina transplantation using major histocompatibility complex (MHC)-homozygote monkey iPSC-retinas in monkeys with laser-induced retinal degeneration in MHC-matched and -mismatched transplantation.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    Single-Cell Transcriptional and Functional Analysis of Dopaminergic Neurons in Organoid-Like Cultures Derived from Human Fetal Midbrain

    The authors used droplet-based single-cell RNA sequencing to transcriptionally profile the developing human ventral midbrain when the dopamine (DA) neurons were generated and their subsequent differentiation into functional mature DA neurons in primary fetal 3D organoid-like cultures.
    [Development]

    Abstract

    iPSCs Derived from Esophageal Atresia Patients Reveal SOX2 Dysregulation at the Anterior Foregut Stage

    To mimic the early development of the esophagus and trachea, investigators differentiated iPSCs from esophageal atresia/tracheoesophageal fistula patients, and iPSCs and ESCs from healthy individuals into mature 3D esophageal organoids.
    [Disease Models & Mechanisms]

    Abstract

    Rewiring Glucose Metabolism Improves 5-FU Efficacy in p53-Deficient/KRASG12D Glycolytic Colorectal Tumors

    Researchers analyzed the 5-fluorouracil (FU) response in patient-derived organoids that reproduced the different stages of colorectal cancer and found that 5-FU induced pyrimidine imbalance, which led to DNA damage and cell death in the actively proliferating cancer cells deficient in p53.
    [Communications Biology]

    Full Article

    A Living Biobank of Canine Mammary Tumor Organoids as a Comparative Model for Human Breast Cancer

    Scientists developed in vitro tools that allowed functional analysis of canine mammary tumors. They established the long-term culture of 24 organoid lines from 16 dogs, including organoids derived from normal mammary epithelium or benign lesions.
    [Scientific Reports]

    Full Article

    3D Human iPSC Blood Vessel Organoids as a Source of Flow-Adaptive Vascular Cells for Creating a Human-Relevant 3D-Scaffold Based Macrovessel Model

    Investigators reported the use of hiPSC-derived vascular organoid cells as a new cell source for the creation of an electrospun polycaprolactone-bisurea 3D-scaffold-based, perfused human macrovessel model.
    [Advanced Biology]

    Full Article

    Stress and Viral Insults Do Not Trigger E200K PrP Conversion in Human Cerebral Organoids

    Isogenically matched cerebral organoids with and without the E200K mutation were used to investigate the propensity of E200K prion protein (PrP) to misfold following cellular insults associated with oxidative stress.
    [PLOS ONE]

    Full Article
    Watch 'collaborating to accelerate COVID-19 research'
    REVIEWS

    In Vitro Generation of Luminal Vasculature in Liver Organoids: From Basic Vascular Biology to Vascularized Hepatic Organoids

    The authors provide an overview of the unique features of hepatic vasculature under pathophysiological conditions and summarize the biochemical and biophysical cues that drive vasculogenesis and angiogenesis in vitro.
    [International Journal of Stem Cells]

    Abstract
    INDUSTRY AND POLICY NEWS

    Medera’s Novoheart Partners with AstraZeneca on First Bioengineered Human Models of Heart Failure

    Medera Biopharmaceutical and Novoheart, together with AstraZeneca, announced that their ongoing collaboration has led to the successful development of the world’s first human-specific in vitro, functional models of heart failure with preserved ejection fraction.
    [Medera Biopharmaceutical Limited (EIN Presswire)]

    Press Release

    Snyder Institute to Uncover New Treatments, Safer Testing Thanks to $35M Donation

    A new $35-million gift left to the Snyder Institute for Chronic Diseases by longstanding donor Dr. Joan Snyder will both sustain the institute in perpetuity and spark new discoveries that build on its world-leading strengths in microbiome and organoid development research.
    [University of Calgary]

    Press Release
    FEATURED EVENT

    Stem Cells: Advances in the Application of Stem Cells and Their Role In Vivo

    February 5 – 8, 2023
    Keystone, Colorado, United States

    > See All Events

    JOB OPPORTUNITIES

    Senior Research Scientist – Pancreatic Organoid Research

    Max Delbrück Center for Molecular Medicine – Berlin, Germany

    Principal Investigator – Lung Organoids

    Helmholtz Munich – Munich, Germany

    Scientist – iPSC and CRISPR

    Icahn School of Medicine at Mount Sinai – New York, New York, United States

    Postdoctoral Researcher – 3D Genome and Molecular Neuroscience

    Helmholtz Munich – Oldenburg, Germany

    Postdoctoral Scientist – Liver Regeneration and Tissue Engineering

    Roger Williams Institute of Hepatology – London, England, United Kingdom

    > See All Jobs

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