DIABETES Otubain 2 Is a Novel Promoter of Beta Cell Survival as Revealed by siRNA High-Throughput Screens of Human Pancreatic Islets Pro-inflammatory cytokines induce death of beta cells and hamper engraftment of transplanted islet mass. Investigators aimed to reveal novel genes involved in this process, as a platform for innovative therapeutic approaches. Small interfering RNA (siRNA) high-throughput screening of primary human islets was employed to identify novel genes involved in cytokine-induced beta cell apoptosis. [Diabetologia] Abstract LIM-Homeodomain Transcription Factor Isl-1 Mediates the Effect of Leptin on Insulin Secretion in Mice Researchers showed that all leptin target cells in pancreatic islets and NIT beta cell express Isl-1. Both in vivo and in vitro results demonstrate that leptin suppresses Isl-1 expression and insulin secretion in islet in physiological and patho-physiological condition e.g., high fat diet. [J Biol Chem] Abstract | Full Article The Adult Mammalian Pancreas Contains Separate Precursors of Pancreatic and Neural Crest Developmental Origins Previously, lineage tracing experiments showed that multipotent precursors in mouse islets had a pancreatic and not a neural crest developmental origin. However, a different Cre reporter system reveals that there is a rare population of proliferative cells in the pancreas that is descended from the Wnt1 neural crest lineage, in addition to the majority population descended from the Pdx1 pancreatic lineage. [Stem Cells Dev] Abstract The Complex Effects of Cannabinoids on Insulin Secretion from Rat Isolated Islets of Langerhans A variety of purported cannabinoid CB receptor agonists and antagonists were evaluated for their effects on insulin secretion. In fresh rat isolated islets, the endocannabinoid anandamide caused a glucose-dependent, concentration-dependent inhibition of insulin release, with two populations of islets being identified based on their sensitivity to anandamide. [Eur J Pharmacol] Abstract Pancreatic Stellate Cells Reduce Insulin Expression and Induce Apoptosis in Pancreatic β-Cells Investigators hypothesized that pancreatic stellate cells (PSCs) might affect the phenotype of pancreatic β-cells. α-Smooth muscle actin (a marker of activated PSC)-positive cells were found within and around the fibrotic islets. [Biochem Biophys Res Commun] Abstract PANCREATIC CANCER Pancreatic Cancer Associated Stellate Cells Promote Differentiation of Myeloid-Derived Suppressor Cells in a STAT3-Dependent Manner Scientists hypothesized that factors produced by human pancreatic stellate cells could enhance myeloid-derived suppressor cell differentiation and function, which promotes an immunosuppressive microenvironment. [Cancer Res] Abstract Palladin Promotes Invasion of Pancreatic Cancer Cells by Enhancing Invadopodia Formation in Cancer-Associated Fibroblasts Researchers utilized immortalized human pancreatic cancer-associated fibroblasts (CAFs) to investigate molecular pathways that control the matrix-remodeling and invasion-promoting activity of CAFs. In mouse xenograft experiments using a mixture of CAFs and tumor cells, palladin expression in CAFs promoted the rapid growth and metastasis of human pancreatic tumor cells. [Oncogene] Abstract | Press Release Six1 Promotes Proliferation of Pancreatic Cancer Cells via Upregulation of Cyclin D1 Expression Scientists showed that the relative expression of Six1 mRNA is increased in pancreatic cancer and correlated with advanced tumor stage. In vitro functional assays demonstrated that forced overexpression of Six1 significantly enhances the growth rate and proliferation ability of pancreatic cancer cells. [PLoS One] Full Article Induction of Pancreatic Cancer Cell Apoptosis, Invasion, Migration, and Enhancement of Chemotherapy Sensitivity of Gemcitabine, 5-FU, and Oxaliplatin by hnRNP A2/B1 siRNA Researchers investigated the effects of inhibiting heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) expression on apoptosis, invasion, migration, and the chemotherapy sensitivity of pancreatic cancer cells to gemcitabine, 5-FU, and oxaliplatin chemotherapy using small interfering RNA (siRNA). [Anticancer Drugs] Abstract |