DIABETES & PANCREATITIS Pdx1 Maintains ß Cell Identity and Function by Repressing an a Cell Program Scientists traced the fate of adult ß cells after Pdx1 deletion. As expected, ß-cell-specific removal of Pdx1 resulted in severe hyperglycemia within days. Surprisingly, a large fraction of Pdx1-deleted cells rapidly acquired ultrastructural and physiological features of a cells, indicating that a robust cellular reprogramming had occurred. [Cell Metab] Abstract | Graphical Abstract Uncoupling of Cav1.2 from Ca2+-Induced Ca2+ Release and SK Channel Regulation in Pancreatic ß-Cells Researchers investigated the role of Cav1.2 in pancreatic ß-cell function by expressing a Cav1.2 II-III loop/GFP fusion in INS-1 cells to disrupt channel-protein interactions. [Mol Endocrinol] Abstract A Critical Role for the Neural Zinc Factor ST18 in Pancreatic Beta-Cell Apoptosis Scientists demonstrated that, in the pancreas, ST18 expression is restricted to endocrine cells. The detection of ST18 expression in pancreatic ß-cells prompted them to investigate its regulation and its role in ß-cell mass and function. [J Biol Chem] Abstract | Full Article Altered Pancreatic Growth and Insulin Secretion in WSB/EiJ Mice Scientists examined whether defects in ß-cell mass or function could contribute to the low insulin levels in WSB mice. WSB mice had markedly increased insulin secretion from isolated islets in vitro. [PLoS One] Full Article Aberrant Islet Unfolded Protein Response in Type 2 Diabetes Researchers analyzed the expression of activating factor 6 and spliced X-box binding protein 1, and phosphorylation of eukaryotic initiation factor 2, to evaluate the three distinct branches of the unfolded protein response in the pancreatic islets of mice with diet- or genetic-induced obesity and insulin resistance. [Sci Rep] Full Article PANCREATIC CANCER Nuclear Translocation of FGFR1 and FGF2 in Pancreatic Stellate Cells Facilitates Pancreatic Cancer Cell Invasion At the invasive front of human pancreatic cancer, FGF2 and FGFR1 localize to the nucleus in activated pancreatic stellate cells (PSCs) but not cancer cells. In vitro, inhibiting FGFR1 and FGF2 in PSCs, using RNAi or chemical inhibition, resulted in significantly reduced cell proliferation, which was not seen in cancer cells. [EMBO Mol Med] Full Article Extracellular Superoxide Dismutase (EcSOD) Suppresses Hypoxia-Inducible Factor-1a (HIF-1a) in Pancreatic Cancer Scientists hypothesized that EcSOD overexpression leads to changes in the O2•-/H2O2 balance modulating the redox status affecting signal transduction pathways. Both transient and stable overexpression of EcSOD suppressed the hypoxic accumulation of HIF-1a in human pancreatic cancer cells. [Free Radic Biol Med] Abstract Antitumor Efficacy of a-Solanine against Pancreatic Cancer In Vitro and In Vivo Researchers investigated the anti-carcinogenic effect of a-solanine against human pancreatic cancer cells. In vitro, a-solanine inhibited proliferation of PANC-1, sw1990, MIA PaCa-2 cells in a dose-dependent manner, as well as cell migration and invasion with atoxic doses. [PLoS One] Full Article Redox-Responsive Targeted Gelatin Nanoparticles for Delivery of Combination wt-p53 Expressing Plasmid DNA and Gemcitabine in the Treatment of Pancreatic Cancer Scientists developed a redox-responsive thiolated gelatin based nanoparticle system that efficiently delivers its payload in the presence of glutathione-mediated reducing intra-cellular environment and could be successfully used for site-specific wt-p53 expressing plasmid DNA as well as gemcitabine delivery by targeting epidermal growth factor receptor. [BMC Cancer] Abstract | Full Article Different Responses of Human Pancreatic Adenocarcinoma Cell Lines to Oncolytic Newcastle Disease Virus Infection In an effort to develop oncolytic virotherapy with Newcastle disease virus (NDV) for patients with pancreatic cancer, scientists evaluated the responses to NDV infection and interferon treatment of 11 different established human pancreatic adenocarcinoma cell lines (HPACs). They showed that all HPACs were susceptible to NDV. [Cancer Gene Ther] Abstract Win a $25 Amazon Gift Card! Connexon Creative is evaluating reader interest in a new social media platform. Fill out this quick survey for your chance to win 1 of 4 $25 gift cards! Take survey here |