DIABETES & PANCREATITIS JunB Protects ß-Cells from Lipotoxicity via the XBP1-AKT Pathway Researchers interrogated the molecular mechanisms involved in JunB-mediated ß-cell protection from lipotoxicity. The saturated fatty acid palmitate decreased JunB expression, and this loss may contribute to ß-cell apoptosis, as overexpression of JunB protected cells from lipotoxicity. [Cell Death Differ] Full Article A Novel Pancreatic ß-Cell Targeting Bispecific-Antibody (BsAb) Can Prevent the Development of Type 1 Diabetes in NOD Mice BsAb was prepared by chemically coupling an anti-glucose transporter-2 (GLUT2) monoclonal antibody (mAb) to an agonistic anti-cytotoxic T-lymphocyte antigen 4 (CTLA-4) mAb. This BsAb was able to bind to GLUT2 and CTLA-4 in vitro, and to pancreatic islets, both in vitro and in vivo. [Clin Immunol] Abstract Aldehyde Dehydrogenase Expression Characterizes G1-Phase Proliferating Beta Cells during Pregnancy Scientists report a dynamic increase in the number of Aldefluor+ beta cells during pregnancy. Interestingly, nearly all these Aldefluor+ beta cells are positive for Ki-67, suggesting that they are in an active cell cycle. [PLoS One] Full Article The H1-Receptor Antagonist Cetirizine Protects Partially against Cytokine- and Hydrogen Peroxide-Induced ß-TC6 Cell Death In Vitro Scientists determined whether H1-receptor antagonists affect cytokine-induced ß-cell death and signaling in vitro. [Pancreas] Abstract PANCREATIC CANCER Chloroquine Targets Pancreatic Cancer Stem Cells via Inhibition of CXCR4 and Hedgehog Signaling Researchers screened established and new compounds for their ability to eliminate cancer stem cells in primary pancreatic cancer cells in vitro and corresponding patient-derived pancreatic cancer tissue xenografts in vivo. [Mol Cancer Ther] Abstract Cellular Contractility and Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity in Pancreatic Cancer Cells Researchers sensitively measured matrix metalloproteinase (MMP) activity changes in response to specific extracellular matrix environments and cell contractility states. Cells of a pancreatic cancer cell line, Panc-1, up-regulate MMP activities between 3- and 10-fold with increased cell contractility. [FASEB J] Abstract PTK6 Promotes Cancer Migration and Invasion in Pancreatic Cancer Cells Dependent on ERK Signaling Scientists evaluated the functional role of protein tyrosine kinase 6 (PTK6) on pancreatic cancer invasion. Five pancreatic cancer cell lines expressed PTK6 at varying levels. PTK6 expression was also observed in human pancreatic adenocarcinomas. [PLoS One] Full Article Pim-3 Promotes Human Pancreatic Cancer Growth by Regulating Tumor Vasculogenesis To assess the role of Pim-3 in human pancreatic carcinogenesis in vivo and to determine the underlying Pim-3 signaling regulatory mechanisms, scientists established MiaPaca-2 cells overexpressing wild-type Pim-3 or Pim-3 kinase dead mutants as well as PCI55 cells stably expressing Pim-3 shRNA or scrambled shRNA in a tetracycline-inducible manner. [Oncol Rep] Abstract |