DIABETES & PANCREATITIS Loss of Fbw7 Reprograms Adult Pancreatic Ductal Cells into α, δ, and β Cells Researchers show that inactivation of the SCF-type E3 ubiquitin ligase substrate recognition component Fbw7 induces pancreatic ductal cells to reprogram into α, δ, and β cells. [Cell Stem Cell] Abstract | Graphical Abstract Comparison Study of Immunohistochemical Staining for the Diagnosis of Type 1 Autoimmune Pancreatitis Specimens of autoimmune pancreatitis, chronic pancreatitis, and pancreatic ductal adenocarcinoma were evaluated with immunohistochemical staining (IHC) for immunoglobulin G (IgG), IgG1, IgG4, and CD138. The number of IHC-positive cells was counted in three, five, and ten different high-power fields by selecting fields with the most numerous positive cells or by randomly selecting fields in the affected areas. [J Gastroenterol] Abstract Calcium Current Inactivation Rather than Pool Depletion Explains Reduced Exocytotic Rate with Prolonged Stimulation in Insulin-Secreting INS-1 832/13 Cells Researchers studied exocytosis, measured as increase in membrane capacitance, as a function of calcium entry in insulin secreting INS-1 832/13 cells using patch clamp and mixed-effects statistical analysis. [PLoS One] Full Article Protective Effects of Lithospermum erythrorhizon against Cerulein-Induced Acute Pancreatitis Pancreatic acinar cells were isolated using a collagenase method, and then researchers investigated the acinar cell viability and cytokine productions. [Pancreas] Abstract Silibinin Protects β Cells from Glucotoxicity through Regulation of the Insig-1/SREBP-1c Pathway Scientists examined whether silibinin protects high glucose-induced β cell dysfunction through the insulin induced gene-1 (Insig-1)/sterol regulatory element binding protein-1c (SREBP-1c) pathway. They demonstrate that 30.0 µM of silibinin significantly improved cell viability after rat insulinoma INS-1 cells were exposed to high glucose for 72 hours. [Int J Mol Med] Abstract PANCREATIC CANCER A Targeting Ligand Enhances Infectivity and Cytotoxicity of an Oncolytic Adenovirus in Human Pancreatic Cancer Tissues Researchers constructed a survivin promoter-regulated conditionally replicative adenovirus (CRAd) expressing enhanced green fluorescent protein gene, which displayed the identified targeting ligand (AdSur-SYE). The AdSur-SYE resulted in higher gene transduction efficiency and oncolytic potency than the untargeted CRAd in several pancreatic cancer cell lines. [J Control Release] Abstract Profilin-1 Suppresses Tumorigenicity in Pancreatic Cancer through Regulation of the SIRT3-HIF1alpha Axis Scientists investigated the exact role of profilin1 (Pfn1) in pancreatic adenocarcinoma and the underlying mechanisms. The effect of Pfn1 expression on cancer proliferation was assessed in cells by up- and down-regulation of Pfn1 in vitro and in vivo. [Mol Cancer] Abstract | Full Article Sphingosine-1-Phosphate Mediates a Reciprocal Signaling Pathway between Stellate Cells and Cancer Cells that Promotes Pancreatic Cancer Growth Sphingosine kinase 1 was overexpressed in human pancreatic tissue, especially within tumor cells. Sphingosine-1-phosphate (S1P) activated pancreatic stellate cells (PSCs) in vitro and conditioned medium from S1P-stimulated PSCs, increased pancreatic cancer cell migration, and invasion, which was dependent on S1P2, c-abl kinase, and matrix metallopeptidase-9. [Am J Pathol] Abstract |