LABORATORY RESEARCH Stress Response Protein RBM3 Attenuates the Stem-Like Properties of Prostate Cancer Cells by Interfering with CD44 Variant Splicing Scientists report that RBM3 is expressed at low basal levels in human fetal prostate or in CD133+ prostate epithelial cells (PrEC), compared to the adult prostate or to CD133- PrEC, and RBM3 is downregulated in cells cultured in soft agar or exposed to stress. Notably, RBM3 overexpression in prostate cancer cells attenuated their stem cell-like properties in vitro as well as their tumorigenic potential in vivo. [Cancer Res] Abstract Expression and Function of the Progesterone Receptor in Human Prostate Stroma Provide Novel Insights to Cell Proliferation Control The authors investigated the expression and function of the progesterone receptor (PR) in the prostate. Two PR isoforms, PRA and PRB, are expressed in prostate stromal fibroblasts and smooth muscle cells, but not in epithelial cells. [J Clin Endocr Metab] Abstract CK2-NCoR Signaling Cascade Promotes Prostate Tumorigenesis Investigators report that both casein kinase 2 (CK2) activity and CK2-mediated NCoR phosphorylation are significantly elevated in the androgen-independent prostate cancer cell line DU145 and PC-3 compared with RWPE1 and LNCaP cells. [Oncotarget] Full Article Differential Androgen Deprivation Therapies with Anti-Androgens of Casodex or MDV3100 vs Anti-Androgen Receptor of ASC-J9 Lead to Promote vs Suppress Prostate Cancer Metastasis Using in vitro and in vivo metastasis models with human prostate cancer (PCa) cell lines, scientists evaluated the effects of the currently used anti-androgens, Casodex and MDV3100, and the newly developed anti-androgen receptor compounds, ASC-J9 and cryptotanshinone, on PCa cell growth and invasion. In vitro results showing that 10 μM Casodex or MDV3100 treatments suppressed PCa cell growth and reduce prostate specific antigen level, yet significantly enhanced PCa cells invasion. [J Biol Chem] Abstract | Full Article Differential Cytotoxic Activity of a Novel Palladium-Based Compound on Prostate Cell Lines, Primary Prostate Epithelial Cells and Prostate Stem Cells Researchers screened the effect of a novel palladium-based anticancer agent (Pd complex) against six different prostate cancer cell lines, and primary cultures from seven Gleason 6/7 prostate cancer, three Gleason 8/9 prostate cancer and four benign prostate hyperplasia patient samples, as well as cancer stem cells selected from primary cultures. The Pd complex showed a powerful growth-inhibitory effect against both cell lines and primary cultures. [PLoS One] Full Article Fate of the Human Y Chromosome Linked Genes and Loci in Prostate Cancer Cell Lines DU145 and LNCaP Researchers screened 51 standard sequence tagged sites corresponding to a male-specific region of the Y chromosome (MSY), sequenced the coding region of the SRY gene and assessed the status of the DYZ1 arrays in the human prostate cancer cell lines DU145 and LNCaP. The MSY was found to be intact and coding region of SRY showed no sequence variation in both the cell lines. [BMC Genomics] Abstract | Full Article BRCA1 and p53 Regulate Critical Prostate Cancer Pathways Considering that p53 is a crucial target in cancer therapy, scientists investigated p53 role in the regulation of transcription of GADD153. They found that GADD153 was highly induced by doxorubicin in prostate cancer cells (PC3) cells; however, this response was totally abolished in LNCaP and in p53-restituted PC3 cells. [Prostate Cancer P D] Abstract Interactional Expression of Netrin-1 and Its Dependence Receptor UNC5B in Prostate Carcinoma To investigate the association of netrin-1 and UNC5B expression with prostate carcinoma, several human prostate carcinoma cell lines were cultured and the expression levels of netrin-1 and UNC5B were determined by real-time PCR and Western blot. Proliferation of DU145 cells was affected by the recruitment of PMA and calphostin C in a dose-dependent manner. [Tumor Biol] Abstract CLINICAL RESEARCH Agent Orange as a Risk Factor for High-Grade Prostate Cancer Scientists determined the association between Agent Orange exposure (AOe) and the risk of detecting high-grade prostate cancer (PCa) on biopsy in a US Veteran cohort. AOe was associated with a 52% increase in the overall risk of detecting PCa. [Cancer] Abstract Significance of Obesity Markers and Adipocytokines in High Grade and High Stage Prostate Cancer in North Indian Men – A Cross-Sectional Study The authors investigated the role of adipocytokines in stimulating the promotion and progression of prostate cancer (CaP). The level of body mass index, waste to hip ratio, interleukin-6, leptin, and adiponectin were compared between benign prostatic hyperplasia and CaP groups and between grades and stages of prostate cancer. [Cytokine] Abstract |