LABORATORY RESEARCH Aberrant MicroRNA Expression Likely Controls RAS Oncogene Activation during Malignant Transformation of Human Prostate Epithelial and Stem Cells by Arsenic Inorganic arsenic (As) malignantly transforms the RWPE-1 human prostate epithelial line to CAsE-PE cells, and a derivative normal stem cell (SC) line, WPE-stem to As-Cancer SC line. MicroRNAs are non-coding but exert negative control on expression by degradation or translational repression of target mRNAs. [Toxicol Sci] Abstract Morphological Differences between Circulating Tumor Cells from Prostate Cancer Patients and Cultured Prostate Cancer Cells Scientists developed a software tool to investigate the morphological properties of circulating tumor cell from patients with castrate resistant prostate cancer and cultured prostate cancer cells in order to establish whether the latter is an appropriate model for the former. [PLoS One] Full Article Enhanced Shedding of Extracellular Vesicles from Amoeboid Prostate Cancer Cells: Potential Effects on the Tumor Microenvironment Researchers examined the mechanism of release and potential biological functions of extracellular vesicles (EV) shed from DIAPH3-silenced and other prostate cancer cells. They observed that stimulation of LNCaP cells with the prostate stroma-derived growth factor heparin-binding EGF-like growth factor, combined with p38MAPK inhibition caused EV shedding, a process mediated by ERK1/2 hyperactivation. [Cancer Biol Ther] Abstract Human Adipose-Derived Mesenchymal Stromal Cell Pigment Epithelium-Derived Factor Cytotherapy Modifies Genetic and Epigenetic Profiles of Prostate Cancer Cells Researchers examined the influence of adipose-derived mesenchymal stromal cells (ASCs) on gene expression and epigenetic methylation profiles of prostate cancer cells as well as the impact of expressing a therapeutic gene on modifying the interaction between ASCs and prostate cancer cells. [Cytotherapy] Abstract Upregulation of RASGRP3 Expression in Prostate Cancer Correlates with Aggressive Capabilities and Predicts Biochemical Recurrence after Radical Prostatectomy Researchers investigated the expression of guanyl nucleotide-releasing protein for Ras 3 (RasGRP3) in the cell lines and tissues in BPH and prostate cancer, as well as its associations with cancer invasion and prognosis in prostate carcinomas. [Prostate Cancer Prostatic Dis] Full Article MHY-449, a Novel Dihydrobenzofuro[4,5-b][1,8] Naphthyridin-6-One Derivative, Induces Apoptotic Cell Death through Modulation of Akt/FoxO1 and ERK Signaling in PC3 Human Prostate Cancer Cells Scientists examined the growth inhibitory effect of MHY-449 on p53 wild-type LNCaP and p53-null PC3 prostate cancer cells. MHY-449 treatment in androgen-independent and p53-null PC3 cells resulted in inhibition of cell growth and induction of apoptosis in a concentration-dependent manner. [Int J Oncol] Abstract TUSC3 Loss Alters the ER Stress Response and Accelerates Prostate Cancer Growth In Vivo Scientists provide evidence that tumor suppressor candidate 3 (TUSC3) is part of the oligosaccharyltransferase complex and affects N-linked glycosylation in mammalian cells. Loss of TUSC3 expression in DU145 and PC3 prostate cancer cell lines leads to increased proliferation, migration and invasion as well as accelerated xenograft growth in a PTEN negative background. [Sci Rep] Full Article CLINICAL RESEARCH Phase I/II Trial of Orteronel (TAK-700) – An Investigational 17,20-Lyase Inhibitor – In Patients with Metastatic Castration-Resistant Prostate Cancer Researchers conducted a Phase I/II study in men with progressive, chemotherapy-naïve, metastatic castration-resistant prostate cancer and serum testosterone < 50 ng/dl. [Clin Cancer Res] Abstract Prostate Cancer Incidence in Males with Lynch Syndrome The purpose of this study was to retrospectively examine prostate cancer incidence in the Lynch syndrome cohort at the Ohio State University in comparison with that in the general population. [Genet Med] Full Article Helical Intensity-Modulated Radiotherapy of the Pelvic Lymph Nodes with Integrated Boost to the Prostate Bed – Initial Results of the PLATIN 3 Trial While the benefit of an additional radiotherapy of the pelvic lymph nodes is still under debate, the PLATIN 3 prospective Phase II clinical trial was initiated to substantiate toxicity data on postoperative intensity-modulated radiotherapy of the pelvic lymph nodes and the prostate bed. [BMC Cancer] Full Article |