Pulmonary Cell News Volume 10.10 | Mar 18 2021

May 12, 2022

2021-03-18 | PULCN 10.10

Pulmonary Cell News by STEMCELL Technologies

Vol. 10.10 – 18 March, 2021

TOP STORY

SARS-CoV-2 Vaccine ChAdOx1 nCoV-19 Infection of Human Cell Lines Reveals Low Levels of Viral Backbone Gene Transcription Alongside Very High Levels of SARS-CoV-2 S Glycoprotein Gene Transcription

Researchers used direct RNA sequencing to analyze transcript expression from the ChAdOx1 nCoV-19 genome in human MRC-5 and A549 cell lines that were non-permissive for vector replication alongside the replication permissive cell line, HEK293. They also used quantitative proteomics to study over time the proteome and phosphoproteome of A549 and MRC5 cells infected with the ChAdOx1 nCoV-19 vaccine.
[Genome Medicine]

Full Article

Grow Functional Airway Organoids with PneumaCult™

PUBLICATIONSRanked by the impact factor of the journal

Targeting the AXL Receptor in Combating Smoking-Related Pulmonary Fibrosis

Using a phospho-receptor tyrosine kinase (RTK) array screen, investigators identified the RTK, AXL, as a top upregulated RTK in response to smoke. Both expression and signaling activity of AXL were elevated in lung fibroblasts exposed to tobacco-smoke, whereas no significant change to the levels of a canonical AXL ligand, growth arrest-specific 6, was seen upon smoke treatment.
[American Journal of Respiratory Cell and Molecular Biology]

Abstract

Human Pluripotent Stem-Cell-Derived Alveolar Organoids for Modeling Pulmonary Fibrosis and Drug Testing

Scientists generated alveolar organoids derived from human pluripotent stem cells for use as an pulmonary fibrosis model and for drug efficacy evaluation.
[Cell Death Discovery]

Full Article

Assisting PNA Transport through Cystic Fibrosis Human Airway Epithelia with Biodegradable Hybrid Lipid-Polymer Nanoparticles

Investigators loaded hybrid core–shell nanoparticles (hNPs) with a 7-mer peptide nucleic acid (PNA) previously considered for its ability to modulate the post-transcriptional regulation of the CF transmembrane conductance regulator gene. They also investigated the in vitro release kinetics of hNPs and their efficacy in PNA delivery across the human epithelial airway barrier using an ex vivo model based on human primary nasal epithelial cells from CF patients.
[Scientific Reports]

Full Article

Exposure of Human Lung Cells to Polystyrene Microplastics Significantly Retards Cell Proliferation and Triggers Morphological Changes

To examine the potential toxicological effects of microplastics on human cells, the cultured human alveolar A549 cells were exposed to polystyrene microplastics of one and ten μm diameter as a model of the environmental contaminants.
[Chemical Research in Toxicology]

Abstract Graphical Abstract

LUNG CANCER

Topological Signatures in Regulatory Network Enable Phenotypic Heterogeneity in Small Cell Lung Cancer

The authors investigated a regulatory network underlying phenotypic heterogeneity in SCLC, a devastating disease with no molecular targeted therapy.
[eLife]

Abstract

Musashi-2 (MSI2) Regulates Epidermal Growth Factor Receptor (EGFR) Expression and Response to EGFR Inhibitors in EGFR-Mutated Non-Small Cell Lung Cancer (NSCLC)

MSI2 control of EGFR expression and activity in an NSCLC cell line panel was studied using RT-PCR, Western blots, and RNA immunoprecipitation. Functional consequences of MSI2 depletion were explored for cell growth and response to EGFR-targeting drugs, in vitro and in vivo.
[Oncogenesis]

Full Article

Splicing Reprogramming of TRAIL/DISC-Components Sensitizes Lung Cancer Cells to TRAIL-Mediated Apoptosis

Scientists revealed that apigenin directed alternative splicing and inhibited heat shock protein 70 enhancing tumor necrosis factor-related apoptosis-inducing ligand anticancer activity.
[Cell Death & Disease]

Full Article

Punicalagin Induces ROS‐Mediated Apoptotic Cell Death through Inhibiting STAT3 Translocation in Lung Cancer A549 Cells

Researchers evaluated the antiproliferative ability of punicalagin against lung cancer A549 cells by inducing apoptosis through inhibiting STAT‐3 activation.
[Oncogenesis]

Abstract

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REVIEWS

Role of Extracellular Vesicles in Chronic Lung Disease

The authors provide a broad overview of the roles of extracellular vesicles (EV) in chronic respiratory disease. Recent advances in profiling EVs have shown their potential as biomarker candidates. Studies have provided insight into their disease pathology, particularly in inflammatory processes across a spectrum of lung diseases.
[Thorax]

Full Article

Uncommon Targets in Non-Small Cell Lung Cancer: Everyone Wants a Slice of Cake

Scientists discuss some uncommon alterations in NSCLC such as ROS1, BRAF, RET, HER2, NTRK, MET and other targets that are in an early evaluation phase. They also summarize the characteristics of patients harboring these alterations, the already approved or under investigation therapies and the related resistance mechanisms.
[Critical Reviews in Oncology/Hematology]

Abstract

INDUSTRY AND POLICY NEWS

Indivumed Announces Expansion into Drug Development, Formation of IX Therapeutics

Indivumed GmbH announced the founding of Ix Therapeutics GmbH, a joint venture with Xlife Sciences AG, which marks Indivumed’s expansion into target discovery and early cancer drug development. Initially, Ix Therapeutics will work with Veraxa Biotech AG to utilize their technology for rapid development of functional therapeutic antibodies, first focusing on colon and lung cancer.
[Indivumed GmbH]

Press Release

FEATURED EVENT

SARS-CoV-2 Vaccine ChAdOx1 nCoV-19 Infection of Human Cell Lines Reveals Low Levels of Viral Backbone Gene Transcription Alongside Very High Levels of SARS-CoV-2 S Glycoprotein Gene Transcription

Targeting the AXL Receptor in Combating Smoking-Related Pulmonary Fibrosis

Human Pluripotent Stem-Cell-Derived Alveolar Organoids for Modeling Pulmonary Fibrosis and Drug Testing

Assisting PNA Transport through Cystic Fibrosis Human Airway Epithelia with Biodegradable Hybrid Lipid-Polymer Nanoparticles

Exposure of Human Lung Cells to Polystyrene Microplastics Significantly Retards Cell Proliferation and Triggers Morphological Changes

Topological Signatures in Regulatory Network Enable Phenotypic Heterogeneity in Small Cell Lung Cancer

Musashi-2 (MSI2) Regulates Epidermal Growth Factor Receptor (EGFR) Expression and Response to EGFR Inhibitors in EGFR-Mutated Non-Small Cell Lung Cancer (NSCLC)

Splicing Reprogramming of TRAIL/DISC-Components Sensitizes Lung Cancer Cells to TRAIL-Mediated Apoptosis

Punicalagin Induces ROS‐Mediated Apoptotic Cell Death through Inhibiting STAT3 Translocation in Lung Cancer A549 Cells

Role of Extracellular Vesicles in Chronic Lung Disease

Uncommon Targets in Non-Small Cell Lung Cancer: Everyone Wants a Slice of Cake

Indivumed Announces Expansion into Drug Development, Formation of IX Therapeutics

British Society of Developmental Biology & Genetics Society Annual Spring Meeting

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