Pulmonary Cell News Volume 10.12 | Apr 1 2021

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    Justin.choi
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    2021-01-04 | PULCN 10.12


    Pulmonary Cell News by STEMCELL Technologies
    Vol. 10.12 – 1 April, 2021
    TOP STORY

    CD148 Deficiency in Fibroblasts Promotes the Development of Pulmonary Fibrosis

    Scientists investigated the role of CD148 in the profibrotic phenotype of fibroblasts in idiopathic pulmonary fibrosis. Conditional CD148 fibroblast-specific knockout mice were generated and exposed to bleomycin, and then assessed for pulmonary fibrosis.
    [American Journal of Respiratory and Critical Care Medicine]

    Abstract

    Tools and products for your COVID-19 research.
    PUBLICATIONSRanked by the impact factor of the journal

    Targeted Replacement of Full-Length CFTR in Human Airway Stem Cells by CRISPR/Cas9 for Pan-Mutation Correction in the Endogenous Locus

    Researchers used CRISPR/Cas9 and two adeno-associated viruses carrying the two halves of the cystic fibrosis transmembrane conductance regulator (CFTR) cDNA to sequentially insert the full CFTR cDNA along with a truncated CD19 enrichment tag in upper airway basal stem cells and human bronchial basal stem cells.
    [Molecular Therapy]

    AbstractGraphical Abstract

    Disparate Temperature-Dependent Virus–Host Dynamics for SARS-CoV-2 and SARS-CoV in the Human Respiratory Epithelium

    Since different temperatures encountered in the human upper and lower respiratory tract have been shown to affect the replication kinetics of several respiratory viruses, as well as host immune response dynamics, the authors investigated the impact of temperatures during SARS-CoV-2 and SARS-CoV infection using the primary human airway epithelial cell culture model.
    [PLoS Biology]

    Full Article

    Incense Smoke-Induced Oxidative Stress Disrupts Tight Junctions and Bronchial Epithelial Barrier Integrity and Induces Airway Hyperresponsiveness in Mouse Lungs

    Using mouse and cell culture models, scientists evaluated the effects of incense smoke exposure on airway hyperresponsiveness, expression of multiple epithelial tight junction- and adherens junction-associated mRNAs and proteins in the lungs, and the barrier function of bronchial epithelial cells assessed by transepithelial electronic resistance.
    [Scientific Reports]

    Full Article

    Cancer-Associated Fibroblasts Activated by miR-196a Promote the Migration and Invasion of Lung Cancer Cells

    miR-196a-overexpressed lung fibroblasts (LF-196a) promoted the migration and invasion of lung cancer cells in co-culture systems. ANXA1 was confirmed as a direct target of miR-196a, and adding back ANXA1 to LF-196a restored the cancer cell invasion promoted by miR-196a.
    [Cancer Letters]

    Abstract

    Loss of the Abasic Site Sensor HMCES Is Synthetic Lethal with the Activity of the APOBEC3A Cytosine Deaminase in Cancer Cells

    The authors report the implementation of CRISPR/Cas-9 genetic screening to identify susceptibilities of multiple A3A-expressing lung adenocarcinoma cell lines.
    [PLoS Biology]

    Full Article

    miR‑454‑3p Inhibits Non‑Small Cell Lung Cancer Cell Proliferation and Metastasis by Targeting TGFB2

    miR‑454‑3p overexpression led to the suppression of proliferation, migration, and invasion in A549 and NCI‑H1650 cells. The overexpression of miR‑454‑3p in A549 and NCI‑H1650 cells significantly inhibited EMT. TGFB2 was revealed to be a direct target of miR‑454‑3p by using TargetScan database and luciferase reporter assay.
    [Oncology Reports]

    Abstract

    Tissue-Specific Transplantation Antigen P35B Functions as an Oncogene and Is Regulated by Microrna-125a-5p in Lung Cancer

    Researchers demonstrated that tissue‑specific transplantation antigen P35B (TSTA3) was regulated by miR‑125a‑5p and functions as an oncogene in lung cancer via promoting the activation of β‑catenin signaling.
    [Oncology Reports]

    Abstract

    Long Noncoding RNA McF2l-AS1 Promotes the Cancer Stem Cell-Like Traits in Non-Small Cell Lung Cancer Cells through Regulating miR-873-5p Level

    Functional experiments showed that MCF2L‐AS1 knockdown suppressed the cancer stem cell‐like traits of non-small cell lung cancer cells through qRT‐PCR, western blot, tumor‐sphere formation, and ALDH activity detection.
    [Environmental Toxicology]

    Abstract

    Zinc‐Finger E‐Box‐Binding Homeobox 1 (ZEB1) Plays a Crucial Role in the Maintenance of Lung Cancer Stem Cells Resistant to Gefitinib

    PC9 and HCC827 non‐small cell lung cancer cell lines were treated with high concentrations of gefitinib, and surviving cells were referred to as “gefitinib‐resistant persisters” (GRPs). Zinc‐finger E‐box‐binding homeobox 1 (ZEB1) knockdown or overexpression was performed to determine the biological significance of ZEB1 in the cancer stem cell features of GRPs, and animal models were studied for in vivo validation.
    [Thoracic Cancer]

    Full Article

    Explore scientific events this April with the Science Events Calendar.
    REVIEWS

    Fusion Proteins in Lung Cancer: Addressing Diagnostic Problems for Deciding Therapy

    The authors explore current diagnostic problems of gene fusion detection relative to the technologies available, in order to clarify future standardization of analyses which determine therapeutic choices.
    [Expert Review of Anticancer Therapy]

    Abstract

    INDUSTRY AND POLICY NEWS

    Roche Receives Positive CHMP Opinion for Tecentriq as a First-Line Monotherapy Treatment for People with a Type of Metastatic Non-Small Cell Lung Cancer

    Roche announced that the European Medicines Agency’s Committee for Medicinal Products for Human Use has recommended the approval of Tecentriq® as a first-line treatment for adults with metastatic non-small cell lung cancer whose tumors have high PD-L1 expression, with no epidermal growth factor receptor or anaplastic lymphoma kinase genomic tumor aberrations.
    [Roche]

    Press Release

    FEATURED EVENT

    Curating the Clinical Genome

    May 12 – 14, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Associate Professor – Xenotransplantation of the Lungs

    Hannover Medical School – Hannover, Germany

    Research Associate – Tissue Systems

    United Therapeutics Corporation – Manchester, New Hampshire, United States

    Postdoctoral Research Fellow – Lung Carcinogenesis

    Thomas Jefferson University – Philadelphia, Pennsylvania, United States

    Director – Pulmonary Biology

    Translate Bio, Inc. – Lexington, Massachusetts, United States

    Postdoctoral Fellow – Lung Injury/Repair

    Brigham and Woman’s Hospital – Boston, Massachusetts, United States

    > See All Jobs

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