MicroRNA-375 Regulation of Thymic Stromal Lymphopoietin by Diesel Exhaust Particles and Ambient Particulate Matter in Human Bronchial Epithelial Cells Scientists showed that diesel exhaust particles (DEP) and ambient fine particulate matter upregulate thymic stromal lymphopoietin (TSLP) mRNA and human microRNA (hsa-miR)-375 in primary human bronchial epithelial cells (pHBEC). Moreover, transfection of pHBEC with anti-hsa-miR-375 reduced TSLP mRNA in DEP but not TNF-α-treated cells. [J Immunol] Abstract miR-145 Regulates Myofibroblast Differentiation and Lung Fibrosis Investigators found that miR-145 expression is upregulated in TGF-β1-reated lung fibroblasts. miR-145 expression is also increased in the lungs of patients with idiopathic pulmonary fibrosis as compared to in normal human lungs. [FASEB J] Abstract Sphingosine-1-Phosphate, FTY720 and Sphingosine-1-Phosphate Receptors in the Pathobiology of Acute Lung Injury Scientists demonstrated that sphingosine 1-phosphate (S1P) is a potent angiogenic factor that enhances lung endothelial cell integrity and an inhibitor of vascular permeability and alveolar flooding in pre-clinical animal models of acute lung injury. [Am J Resp Cell Mol] Abstract A Novel Anti-Inflammatory and Pro-Resolving Role for Resolvin D1 in Acute Cigarette Smoke-Induced Lung Inflammation Primary human lung fibroblasts, small airway epithelial cells and blood monocytes were treated with IL-1β or cigarette smoke extract in combination with resolvin D1 (RvD1) in vitro, production of pro-inflammatory mediators was measured. Investigators report that RvD1 suppressed production of pro-inflammatory mediators by primary human cells in a dose-dependent manner. [PLoS One] Full Article Influenza Matrix Protein 2 Alters CFTR Expression and Function through Its Ion Channel Activity Researchers investigated whether influenza matrix protein (M2), a pH-activated proton (H+) channel that traffics to the plasma membrane of infected cells, altered cystic fibrosis transmembrane conductance regulator (CFTR) expression and function. M2 decreased CFTR activity in: (1) Xenopus oocytes injected with human CFTR; (2) epithelial cells (HEK-293) stably transfected with CFTR; (3) human bronchial epithelial cells (16HBE14o-) expressing native CFTR. [Am J Physiol-Lung C] Abstract Inhibitory Effects of Simvastatin on Staphylococcus aureus Lipoteichoic Acid-Induced Inflammation in Human Alveolar Macrophages Researchers aimed to define the role of Staphylococcus aureus lipoteichoic acid (LTA) in human alveolar macrophages (AMs) and the effects of simvastatin on LTA-stimulated human AMs. They showed that LTA induced tumor necrosis factor-alpha, interleukin(IL)-1beta, IL-8 mRNA expression, and suppressed IL-10 mRNA expression in human AMs. [Clin Exp Med] Abstract LUNG CANCER Upregulation of c-FLIP-short in Response to TRAIL Promotes Survival of NSCLC Cells, Which Could Be Suppressed by Inhibition of Ca2+/Calmodulin Signaling Scientists report that TNF-related apoptosis-inducing ligand (TRAIL) rapidly activates caspase-8 in a panel of non-small-cell lung carcinomas (NSCLCs). The results revealed that the upregulation of DR4 and caspase-8 expression in NSCLC cells make them more susceptible to TRAIL. However, these cells could survive TRAIL treatment via upregulation of cellular-FLIP-short (c-FLIPS), and it is suggested that blocking c-FLIPS expression by inhibition of Ca2+/calmodulin signaling significantly overcomes the acquired resistance of NSCLC cells to TRAIL. [Cell Death Dis] Full Article MAPK p38 and JNK have Opposing Activities on TRAIL-Induced Apoptosis Activation in NSCLC H460 Cells that Involves RIP1 and Caspase-8 and Is Mediated by Mcl-1 Scientists examined the consequences and mechanisms of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced MAPKs, p38 and JNK in non-small cell lung cancer (NSCLC) cells. In apoptosis sensitive H460 cells, these kinases were phosphorylated, but not in resistant A549 cells. [Apoptosis] Abstract Human Lung Epithelial Cells Progressed to Malignancy Through Specific Oncogenic Manipulations Investigators used CDK4/hTERT-immortalized normal human bronchial epithelial cells (HBECs) from several individuals to study lung cancer pathogenesis by introducing combinations of common lung cancer oncogenic changes (p53, KRAS, MYC) and followed the stepwise transformation of HBECs to full malignancy. [Mol Cancer Res] Abstract Differential Effects of Mesenchymal Stem Cells on a Heterogeneous Cell Population within Lung Cancer Cell Lines The effects of mesenchymal stem cells (MSCs) on the growth and microsphere formation of heterogeneous cell populations within two lung cancer cell lines (A549 and H446) were compared. Researchers report that MSCs inhibited the in vitro proliferation of both cell lines, but significantly accelerated tumor formation and stimulated tumor growth in vivo. [Mol Cell Biochem] Abstract |