Pulmonary Cell News Volume 2.24 | Jun 20 2013

Lung-Resident CD4⁺ T Cells Are Sufficient for Interleukin-4Rα-Dependent Recall Immunity to Nippostrongylus brasiliensis Infection
Immunity to Nippostrongylus brasiliensis reinfection requires pulmonary CD4⁺ T-cell responses. Investigators examined whether secondary lymphoid recruited or pre-existing lung CD4⁺ T-cell populations coordinated this immunity. [Mucosal Immunol] Abstract

Inflammation Promotes Airway Epithelial ATP Release via Calcium-Dependent Vesicular Pathways
Scientists investigated links between cystic fibrosis (CF)-associated airway inflammation and airway epithelial ATP release. Primary human bronchial epithelial (HBE) cells isolated from CF lungs exhibited enhanced IL-8 secretion after 6-11 days, but not 28-35 days, in culture, relative to normal HBE cells. [Am J Respir Cell Mol Biol] Abstract

Chemokine Localization in Bronchial Angiogenesis
Scientists analyzed left lung bronchoalveolar lavage fluid and left bronchus homogenates after left pulmonary artery ligation in rats, immediately after the onset of ischemia, 6 hours and 24 hours later. Additionally, they tested the effectiveness of dexamethasone on decreasing inflammation and angiogenesis at early and late stages. [PLoS One] Full Article

Reduced Chromosome Aberration Complexity in Normal Human Bronchial Epithelial Cells Exposed to Low-LET γ-Rays and High-LET α-Particles
Scientists exposed normal human bronchial lung epithelial cells in vitro to 0.5 and 1 Gy low-linear energy transfer (LET) γ-rays and a low fluence of high-LET α-particles and assayed for chromosome aberrations in premature chromosome condensation spreads by 24-color multiplex-fluorescence in situ hybridization. Both simple and complex aberrations were induced in a LET and dose-dependent manner. [Int J Radiat Biol]
Full Article

In-Cell Western™ Detection of Organic Cation Transporters in Bronchial Epithelial Cell Layers Cultured at an Air-Liquid Interface on Transwell^® Inserts
Researchers designed an in-cell Western™ assay for measuring levels of the different organic cation transporters (OCT) subtypes in intact layers of the human bronchial epithelial Calu-3 cell line cultured at an air-liquid interface on Transwell^® inserts. The protocol was subsequently validated by exposing cell layers to compounds known to cause variations in OCT expression. [J Pharmacol Toxicol Methods] Abstract

Rhythmic Pressure Waves Induce Mucin5AC Expression via an EGFR-Mediated Signaling Pathway in Human Airway Epithelial Cells
Researchers tested the effect and mechanism of rhythmic pressure waves on mucin5AC (MUC5AC) expression in cultured human bronchial epithelial cells. Compared with the relevant controls, the transcriptional level of MUC5AC and the protein expressions of MUC5AC, the phospho-epidermal growth factor receptor, phospho-extracellular signal-related kinase, and phospho-Akt were all significantly increased after mechanical stimulation. [DNA Cell Biol] Abstract

LUNG CANCER

Gene Amplification of the Histone Methyltransferase SETDB1 Contributes to Human Lung Tumorigenesis
Researchers showed that the histone methyltransferase SETDB1 undergoes gene amplification in non-small and small lung cancer cell lines and primary tumors. The existence of additional copies of the SETDB1 gene in these transformed cells is associated with higher levels of the corresponding mRNA and protein. [Oncogene] Abstract

Pulmonary Fibroblasts Induce Epithelial Mesenchymal Transition and Some Characteristics of Stem Cells in Non-Small Cell Lung Cancer
Fibroblasts isolated from surgical exploration were co-cultured with human lung adenocarcinoma cell lines. Expression levels of myofibroblast markers were higher in cancer associated fibroblasts than lung normal fibroblasts within five passages in the absence of continuing interaction with carcinoma cells. [Ann Thorac Surg] Full Article

Ascorbic Acid and a Cytostatic Inhibitor of Glycolysis Synergistically Induce Apoptosis in Non-Small Cell Lung Cancer Cells
Scientists compared the effects of ascorbic acid (AA) and combinations of AA with the glycolysis inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen​-1-one(3-PO) on the viability of three non-small cell lung cancer (NSCLC) cell lines to the effects on an immortalized lung epithelial cell line. AA concentrations of 0.5 to 5 mM caused a complete loss of viability in all NSCLC lines compared to a <10% loss of viability in the lung epithelial cell line. [PLoS One] Full Article

Interleukin-22 Is Frequently Expressed in Small- and Large-Cell Lung Cancer and Promotes Growth in Chemotherapy-Resistant Cancer Cells
Investigators analyzed the cellular effects of interleukin-22 (IL-22) on lung carcinoma cell lines and the prognostic impact of IL-22 tissue expression in lung cancer patients. They found the IL-22 receptor 1 to be expressed in six of seven lung cancer cell lines. [J Thorac Oncol] Abstract 

New Anti-Inflammatory Targets for Chronic Obstructive Pulmonary Disease
This review discusses some of the most promising of these targets, including new antioxidants, kinase inhibitors and drugs that target cellular senescence, microbial colonization, epigenetic regulation of inflammatory gene expression and corticosteroid resistance. [Nat Rev Drug Discov] Abstract

Visit our reviews page to see a complete list of reviews in the pulmonary cell research field.

Teva Strengthens Respiratory Franchise with Acquisition of MicroDose Therapeutx
Teva Pharmaceutical Industries Ltd. announced that it has entered into a definitive agreement to acquire MicroDose Therapeutx, a privately-held pharmaceutical and drug delivery company focused on inhalation technologies and products for lung diseases and infections with the potential to dramatically improve efficacy and patient compliance. [Teva Pharmaceutical Industries Ltd.] Press Release

Sanovas and Mayo Clinic Enter Joint Development Agreement to Create Technologies to Treat Asthma
Sanovas, Inc. announced that it has entered into a patent license and joint development agreement with Mayo Clinic to commercialize a significant innovation in the emerging science of bronchial smooth muscle modification. [Sanovas, Inc.] Press Release

DiaTech Oncology Test Shows Positive Results, Identifies New Therapeutic Strategies for Patients with Lung Cancer and Ovarian Cancer
DiaTech Oncology has developed a predictive test that has been effective in clinical trials in helping oncologists determine the best chemotherapy treatment plans and therapeutic strategies for more than 50 types of cancers. The company has just published detailed results of the clinical trials for mesothelioma lung cancer, small-cell lung cancer and advanced stage III and IV ovarian cancer. [DiaTech Oncology]
Press Release

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National Institutes of Health (United States)

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

NEW Cold Spring Harbor Laboratory: Stem Cell Biology
September 24-28, 2013
Cold Spring Harbor, United States

Visit our events page to see a complete list of events in the pulmonary cell community.

Director of Cell Processing Facility (S L Collins Associates, Inc.)

Postdoctoral Associate – Mechanisms of Pathogenesis in Idiopathic Pulmonary Fibrosis (University of Minnesota)

Postdoctoral Position – Idiopathic Pulmonary Fibrosis (MedImmune UK Ltd)

Postdoctoral Position – Lung Development and Repair (Boston University Pulmonary Center)

PhD Studentships – Pulmonary Biology and Medicine (University of Giessen and Marburg Lung Center)

Postdoctoral Fellow – Stem Cell and Cancer Biology (Johns Hopkins University School of Medicine)

Postdoctoral Position – Cardiopulmonary Molecular Biology (Hannover Medical School)

PhD Opportunity – Biofunctionalization of Liposomes for Tumor-Targeted Drug Delivery (University of East Anglia)

Postdoctoral Position – Pulmonary Research (Cedars-Sinai Medical Center)

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