Anoctamin 1 Dysregulation Alters Bronchial Epithelial Repair in Cystic Fibrosis Scientists showed, with human bronchial epithelial cell lines and primary cells, that both cell proliferation and migration during epithelial repair are delayed in cystic fibrosis (CF) compared to non-CF cells. [Biochim Biophys Acta] Abstract Smoking Exposure Induces Human Lung Endothelial Cells Adaptation to Apoptotic Stress Researchers modeled paracrine increases of palmitoyl ceramide (Cer16) in primary human lung microvascular cells. In naïve cells, isolated from non-smokers, Cer16 significantly reduced cellular proliferation and induced caspase-independent apoptosis via mitochondrial membrane depolarization, AIF translocation, and PARP cleavage. [Am J Respir Cell Mol Biol] Abstract Pro-Inflammatory Cytokine Induction of 11β-Hydroxysteroid Dehydrogenase Type 1 in A549 Cells Requires Phosphorylation of C/EBPβ at Thr235 Researchers showed 11β-hydroxysteroid dehydrogenase type 1 expression in human A549 epithelial cells is increased by pro-inflammatory cytokines via the P2 promoter of the HSD11B1 gene. [PLoS One] Full Article Establishment and Evaluation of a Stable Cattle Type II Alveolar Epithelial Cell Line The cattle type II alveolar epithelial cell line that researchers established retained lung epithelial cell characteristics: the cells were positive for surfactants A and B, and they secreted tumor necrosis factor-α and interleukin-6 in response to bacterial invasion. [PLoS One] Full Article Carbocysteine Regulates Innate Immune Responses and Senescence Processes in Cigarette Smoke Stimulated Bronchial Epithelial Cells Researchers explored whether carbocysteine, in a human bronchial epithelial cell line, counteracted some pro-inflammatory cigarette smoke extracts-mediated effects. [Toxicol Lett] Abstract Highly Purified, Multi-Wall Carbon Nanotubes Induce Light-Chain 3B Expression in Human Lung Cells The authors focused on the autophagic responses of HTT2800 exposure. The HTT2800-exposed cells induced LC3B expression and induced cell growth inhibition. [Biochem Biophys Res Commun] Abstract LUNG CANCER CRL4A-FBXW5-Mediated Degradation of DLC1 Rho GTPase-Activating Protein Tumor Suppressor Promotes Non-Small Cell Lung Cancer Cell Growth DLC1 encodes a RhoA GTPase-activating protein and tumor suppressor lost in cancer by genomic deletion or epigenetic silencing and loss of DLC1 gene transcription. Scientists unexpectedly identified non-small cell lung cancer cell lines and tumor tissue that expressed DLC1 mRNA yet lacked DLC1 protein expression. [Proc Natl Acad Sci USA] Abstract Uptake, p53 Pathway Activation and Cytotoxic Responses for Co(II) and Ni(II) in Human Lung Cells: Implications for Carcinogenicity Cobalt(II) and nickel(II) ions display similar chemical properties and act as hypoxia mimics in cells. However, only soluble Co(II) but not soluble Ni(II) is carcinogenic by inhalation. To explore potential reasons for these differences, scientists examined responses of human lung cells to both metals. [Toxicol Sci] Abstract miR-205 Promotes the Growth, Metastasis and Chemoresistance of NSCLC Cells by Targeting PTEN Researchers identified the promotive effects of microRNA (miR)-205 on various significant biological properties of non-small cell lung cancer (NSCLC) cells, and confirmed the regulation of PTEN by miR-205. [Oncol Rep] Abstract Application of Proteomics to Identify the Target Molecules Involved in Lonicera japonica-Induced Photokilling in Human Lung Cancer CH27 Cells Mitochondria and endoplasmic reticulum are the integral targets in photoactivated Lonicera japonica-induced CH27 cell apoptosis. Researchers also demonstrated that ethyl acetate fraction of Lonicera japonica extracts caused photocytotoxicity in a dose-dependent manner in CH27 cells. [BMC Complement Altern Med] Abstract | Full Article |