Pulmonary Cell News Volume 2.45 | Nov 14 2013

Anchored PDE4 Regulates Chloride Conductance in Wild-Type and ?F508-CFTR Human Airway Epithelia
Scientists provide evidence that type 4 cyclic nucleotide phosphodiesterases (PDE4s) are critical regulators of the cAMP/PKA-dependent activation of cystic fibrosis transmembrane conductance regulator (CFTR) in primary human bronchial epithelial cells. [FASEB J] Abstract

Loss of Hypoxia-Inducible Factor 2 Alpha (HIF2a) in the Lung Alveolar Epithelium of Mice Leads to Enhanced Eosinophilic Inflammation in Cobalt-Induced Lung Injury
Scientists hypothesized that loss of HIF2a in lung epithelium would provide protection from cobalt-induced inflammation. Mice with HIF2a-deficiency in Club and alveolar type II epithelial cells were exposed to cobalt or saline using a subacute occupational exposure model. [Toxicol Sci] Abstract

Breaking the In-Vitro Alveolar Type II Cell Proliferation Barrier While Retaining Ion Transport Properties
Researchers demonstrated that a novel co-culture technique breaks the in vitro alveolar type II cell proliferation barrier while retaining cell-specific functional properties. [Am J Respir Cell Mol Biol] Abstract

Internalization and Trafficking of Nontypeable Haemophilus influenzae in Human Respiratory Epithelial Cells and the Roles of IgA1 Proteases for Optimal Internalization and Persistence
Researchers showed that nontypeable Haemophilus influenzae invades human bronchial epithelial cells in vitro in a lipid raft- independent manner, is subsequently trafficked via the endolysosomal pathway, and is killed in lysosomes after variable durations of persistence. [Infect Immun] Abstract

LysoTracker Is a Marker of Differentiated Alveolar Type II Cells
LysoTrackerpos alveolar type II (AT2) cells generated SP-Cpos alveolar epithelial cell colonies in culture, and when added to the epithelial colony-forming unit culture medium, LysoTracker marked the differentiation of stem/progenitor-derived AT2 cells. [Respir Res] Abstract | Full Article

LUNG CANCER

Foxm1 Transcription Factor Is Required for the Initiation of Lung Tumorigenesis by Oncogenic Kras^G12D
Researchers used transgenic mice expressing Kras^G12D under control of Sftpc promoter to demonstrate that Foxm1 was induced in type II epithelial cells before the formation of lung tumors. [Oncogene] Abstract

Glycogen Synthase Kinase 3 Alpha and Beta Are New Functionally Relevant Targets of Tivantinib in Lung Cancer Cells
Consistently, in determining tivantinib’s activity profile in a broad panel of non-small cell lung cancer cell lines, scientists found that, in contrast to several more potent c-MET inhibitors, tivantinib reduces cell viability across most of these cell lines. [ACS Chem Biol] Abstract

Sequential Targeted Delivery of Pacilitaxel and Camptothecin Using a Crosslinked ‘Nanosponge’ Network for Lung Cancer Chemotherapy
Researchers showed significant G2/M phase cell cycle arrest, changes in microtubule dynamics that produced increased apoptotic cell death and decreased proliferation with initial exposure to nanoparticle (NP) paclitaxel followed by NP camptothecin in lung cancer cells. [Mol Pharm] Abstract

Extract of Bryophyllum laetivirens Reverses Etoposide Resistance in Human Lung A549 Cancer Cells by Downregulation of NF-?B
Researchers attempted to identify a new and effective anticancer drug against multidrug resistant cancer cells. They first found that lung cancer A549 cells resistant to etoposide exhibit upregulation of NF-?B and SIRT1 in comparison to A549 parental cells. [Oncol Rep] Abstract

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Foundation Medicine Partners with Broad-Based Coalition to Advance Revolutionary New Clinical Trial Design in Lung Cancer
Foundation Medicine, Inc. announced that it has partnered with Friends of Cancer Research, as well as federal health and regulatory agencies, pharmaceutical companies, multiple cooperative groups and patient advocacy organizations to develop the clinical trial design for the “Master Protocol” study in patients with squamous cell carcinoma of the lung. [Foundation Medicine, Inc.] Press Release

Discovery Labs Receives FDA Clearance of IND to Initiate AEROSURF® Phase II Clinical Program
Discovery Laboratories, Inc. announced that the U.S. Food and Drug Administration (FDA) has cleared the Company’s investigational new drug (IND) application for AEROSURF® and the Company can initiate its Phase II clinical program. [Discovery Laboratories, Inc.] Press Release

Boehringer Ingelheim Submits Applications in Europe to Extend the Indication for the Use of Tiotropium Respimat® to the Treatment of Asthma in Adults Aged 18 Years and Over
Boehringer Ingelheim announced submission of applications to extend the indication for the use of tiotropium Respimat® to the treatment of asthma in adults aged 18 years and over. [Boehringer Ingelheim GmbH] Press Release

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Postdoctoral Fellow – Respiratory Research (Karolinska Institutet)

PhD Studentship – Epithelial Surfaces in the Lung (University of East Anglia)

Postdoctoral Scientist – Prognostic Biomarkers in Lung Cancer and Mesothelioma (Medical Research Council)

Postdoctoral Research Fellow – Emphysema (University College Dublin)

Postdoctoral Research Associate – Airway Innate Defense and Biology (University of North Carolina – Chapel Hill)

Senior Postdoctoral Fellow – Mechanisms of Lung Injuries (University of Texas Health Science Center at Tyler)

Scientist – Particle Chemistry (STEMCELL Technologies Inc.)

Research Technologist – Human Pluripotent Stem Cell Products (STEMCELL Technologies Inc.)

Research Associate/Scientist – Antibodies Group (STEMCELL Technologies Inc.)

Research Associate – Particle Chemistry (STEMCELL Technologies Inc.)

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