A Paracrine Network Regulates the Cross-Talk between Human Lung Stem Cells and the Stroma Scientists defined molecular mechanisms involved in the interaction between human lung Lgr6+ stem cells and fibroblasts in a functional microenvironment. They revealed a central role for p38a MAPK in establishing and maintaining such cross-talk, acting in both cell types. [Nat Commun] Full Article A Site-Specific Genetic Modification for Induction of Pluripotency and Subsequent Isolation of Derived Lung Alveolar Epithelial Type II Cells Researchers report a novel strategy using a single nonviral site-specific targeting vector with a combination of Tet-On inducible gene expression system, Cre/lox P switching gene expression system, and alveolar epithelial type II cell-specific NeomycinR transgene expression system. [Stem Cells] Abstract Chronic Arsenic Exposure and Angiogenesis in Human Bronchial Epithelial Cells via the ROS/miR-199a-5p/HIF-1a/COX-2 Pathway Researchers utilized an in vitro model by transforming human lung epithelial BEAS-2B cells through long-term exposure to arsenic. They found that miR-199a-5p expression levels were more than 100-fold lower in arsenic-transformed cells than parental cells. [Environ Health Perspect] Abstract Expression of Caveolin-1 and Podocalyxin in Rat Lungs Challenged with 2-kDa Macrophage-Activating Lipopeptide and Flt3L Researchers examined the immunohistochemical expression of caveolin-1 and podocalyxin in lungs from rats challenged with a 2-kDa macrophage-activating lipopeptide and Fms-like tyrosine kinase receptor-3 ligand (Flt3L). Normal rat lungs expressed caveolin-1 in alveolar septa, vascular endothelium and airway epithelium, especially along the lateral borders of epithelial cells but not in alveolar macrophages. [Cell Tissue Res] Abstract Chemoprotective Effect of Monoisoamyl 2, 3-Dimercaptosuccinate (MiADMS) on Cytokines Expression in Cadmium Chloride Treated Human Lung Cells The chemoprotective effect of MiADMS was evaluated on viability and cytokines expression in CdCl2 treated human lung A549 cells by cytokine array. The CdCl2 caused a dose dependent decrease in cell viability, while MiADMS co-treatment resulted in a significant increase in viability of CdCl2 treated cells. [Environ Toxicol] Abstract LUNG CANCER Receptor-Interacting Protein 1 Increases Chemoresistance by Maintaining Inhibitor of Apoptosis Protein Levels and Reducing Reactive Oxygen Species through a MicroRNA-146a-Mediated Catalase Pathway Researchers found that in human lung cancer cells, knockdown of receptor-interacting protein 1 substantially increased cytotoxicity induced by frontline anticancer therapeutic cisplatin, which was associated with robust cellular reactive oxygen species accumulation and enhanced apoptosis. [J Biol Chem] Abstract Inhibitory Effect of Dihydroaustrasulfone Alcohol on the Migration of Human Non-Small Cell Lung Carcinoma A549 Cells and the Antitumor Effect on a Lewis Lung Carcinoma-Bearing Tumor Model in C57BL/6J Mice Researchers discovered that dihydroaustrasulfone alcohol provided a concentration-dependent inhibitory effect on the migration and motility of human non-small cell lung carcinoma A549 cells by trans-well and wound healing assays. [Mar Drugs] Abstract | Full Article Gambogenic Acid Kills Lung Cancer Cells through Aberrant Autophagy Researchers found that gambogenic acid (GNA) could induce the formation of vacuoles, which was linked with autophagy in A549 and HeLa cells. Further studies revealed that GNA triggers the initiation of autophagy based on the results of MDC staining, AO staining, accumulation of LC3 II, activation of Beclin 1 and phosphorylation of P70S6K. [PLoS One] Full Article Inhalable Microspheres Embedding Chitosan-Coated PLGA Nanoparticles for 2-Methoxyestradiol Using soluble excipients as microspheres (MS) matrix, respirable MS embedding chitosan-coated poly(D,L-lactide-co-glycolide) nanoparticles (CNP-MS) for 2-methoxyestradiol (2-ME) were designed, which could avoid macrophage phagocytosis to achieve the targeted delivery of these drugs. CNP-MS markedly enhanced the cytotoxicity of 2-ME by approximately 8.8-fold and 3.65-fold on SPC-A1 cells compared to solution and NP, respectively. [J Drug Target] Abstract Methylated +58CpG Site Decreases DCN mRNA Expression and Enhances TGF-ß/Smad Signaling in NSCLC Cells with High Metastatic Potential The epigenetic mechanisms by which defective expression of decorin (DCN) causes cancer metastasis remain unclear. Researchers focused on non-small cell lung cancer (NSCLC) cell lines with low metastatic potential and high metastatic potential, which share a similar genetic background. [Int J Oncol] Abstract |