Double Impact of Cigarette Smoke and Mechanical Ventilation on the Alveolar Epithelial Type II Cell As alveolar type II cells are central to maintaining the alveolar epithelial barrier during oxidative stress, mediated in part by neutrophilic inflammation and mechanical ventilation, scientists hypothesized that exposure to cigarette smoke and mechanical strain have interactive effects leading to the activation of and damage to alveolar type II cells. [Crit Care] Abstract | Full Article Whole-Genome Analysis of Temporal Gene Expression during Early Transdifferentiation of Human Lung Alveolar Epithelial Type 2 Cells In Vitro A discovery-based gene expression profile analysis was undertaken of freshly isolated human alveolar epithelial type II (hAT2) cells grown on extracellular matrix substrata known to either support or retard the early transdifferentiation process into hAT1-like cells over the first three days. [PLoS One] Full Article Conditioned Media from Mesenchymal Stromal Cells Restores Sodium Transport and Preserves Epithelial Permeability in an In Vitro Model of Acute Alveolar Injury To determine the mechanisms by which mesenchymal stromal cells media exerts its beneficial effect, an in vitro model of alveolar epithelial injury was created by exposing primary rat alveolar epithelial cells to hypoxia plus cytomix, a combination of IL-1ß, TNF-a and IFN-?. [Am J Physiol Lung Cell Mol Physiol] Abstract mAChRs Activation Induces Epithelial-Mesenchymal Transition on Lung Epithelial Cells Decreased E-cadherin expression and increased vimentin and alpha-SMA expression induced by TGF-beta1 in alveolar epithelial cells were significantly abrogated by the non-selective mAChR antagonist atropine and enhanced by the acetylcholinesterase inhibitor physostigmine. [BMC Pulm Med] Abstract | Full Article Blackcurrant Cultivar Polyphenolic Extracts Suppress CCL26 Secretion from Alveolar Epithelial Cells Researchers evaluated the effectiveness of ten different blackcurrant cultivar polyphenolic extracts in suppressing CCL26 secretion in stimulated human alveolar epithelial cells. [Food Funct] Abstract Isolation and Cultivation of Metabolically Competent Alveolar Epithelial Cells from A/J Mice To enable mechanistic investigations the isolation and cultivation of alveolar epithelial cells (AECs) is desirable. Based on four different protocols dispase digestion of lung tissue was best and yielded 9.3±1.5 x 106 AECs. [Toxicol In Vitro] Abstract LUNG CANCER KRAS Mutation Status Is Associated with Enhanced Dependency on Folate Metabolism Pathways in Non Small Cell Lung Cancer Cells Scientists explored the possibility of exploiting inherent differences in KRAS mutant cell metabolism for treatment. This study identified a greater dependency on folate metabolism pathways in KRAS mutant compared to KRAS wild type non small cell lung cancer cell lines. [Mol Cancer Ther] Abstract Damaging and Protective Bystander Cross-Talk Between Human Lung Cancer and Normal Cells after Proton Microbeam Irradiation Employing a co-culture approach, scientists investigated the bystander cross-talk between lung cancer and normal cells after proton-microbeam irradiation using ?-H2AX foci fluorescence as a measure of DNA double-strand breaks. [Mutat Res] Abstract Comparative Proteomic Analysis of Anti-Cancer Mechanism by Periplocin Treatment in Lung Cancer Cells The protein profile changes of human lung cancer cell lines A549 in response to periplocin treatment was investigated using a proteomics approach. [Cell Physiol Biochem] Full Article VER-155008, a Small Molecule Inhibitor of HSP70 with Potent Anti-Cancer Activity on Lung Cancer Cell Lines Researchers sought to characterize heat shock protein (HSP)70 and HSP90 as potent regulators of non-small-cell lung cancer (NSCLC) growth. Their results indicate that differential expression of HSP70 is associated with the malignant phenotype of NSCLC cell lines and plays an important regulatory role in NSCLC cell proliferation. [Exp Biol Med] Abstract |