Efficient Bioactive Delivery of Aerosolized Drugs to Human Pulmonary Epithelial Cells Cultured at Air-Liquid Interface Conditions The authors introduce the ALICE-CLOUD technology, which utilizes principles of cloud motion for fast and quantitative delivery of aerosolized liquid drugs to pulmonary cells cultured under realistic air-liquid interface conditions. [Am J Respir Cell Mol Biol] Abstract Lymphocyte-Derived Microparticles Induce Apoptosis of Airway Epithelial Cells through Activation of p38 MAPK and Production of Arachidonic Acid Scientists used bronchial and lung tissue cultures to confirm the pro-apoptotic effects of lymphocyte-derived microparticles (LMPs). In cell culture experiments, they found that LMPs induced human airway epithelial cell apoptosis with associated increases in caspase-3 activity. [Apoptosis] Abstract Brd4 Is Essential for IL-1ß-Induced Inflammation in Human Airway Epithelial Cells Human primary epithelial cells and BEAS-2B cell lines were stimulated with IL-1ß in the presence or absence of H2O2 and the effect of pre-treatment with bromodomain inhibitors was investigated. [PLoS One] Full Article Global Gene Expression Profiling of Human Bronchial Epithelial Cells Exposed to Airborne Fine Particulate Matter Collected from Wuhan, China Scientists aimed to identify genes and pathways that may contribute to particulate matter (PM)2.5-induced lung toxicity in humans through genome-wide approaches. Human bronchial epithelial cells, exposed to various concentrations of PM2.5 collected from Wuhan, China, showed decreased cell viability in a dose-dependent manner. [Toxicol Lett] Abstract Azithromycin Inhibits Double-Stranded RNA-Induced Thymic Stromal Lymphopoietin Release from Human Airway Epithelial Cells Investigators explored effects of azithromycin on dsRNA-induced thymic stromal lymphopoietin in normal human bronchial epithelial cells. [Exp Biol Med] Full Article LUNG CANCER YB-1 Promotes Transcription of Cyclin D1 in Human Non-Small-Cell Lung Cancers Scientists showed YB-1 is involved in the transcription of cyclin D1 in human lung cancer. Depletion of endogenous YB-1 by siRNA inhibited progression of G1 phase and down-regulated both the protein and mRNA levels of cyclin D1 in human lung cancer cells. [Genes Cells] Abstract Antitumor Activity of a Polysaccharide from Longan Seed on Lung Cancer Cell Line A549 In Vitro and In Vivo Researchers showed that water-soluble longan seed polysaccharide had the ability to cause cell cycle arrest in G1 phase, activation of caspases 3 and 9, and cleavage of poly[ADP (ribose)] polymerase in A549 cells. [Tumor Biol] Abstract Stat3 Inhibits Beclin 1 Expression through Recruitment of HDAC3 in Nonsmall Cell Lung Cancer Cells Researchers demonstrated that Beclin 1 is a direct transcriptional target of signal transducer and activator of transcription 3 (Stat3) in lung cancer cells. Interleukin-6 treatment or transfection of a constitutively activated Stat3 in AGS and NCI-H1650 cells inhibited Beclin 1 expression. [Tumor Biol] Abstract Auricularia polytricha Polysaccharides Induce Cell Cycle Arrest and Apoptosis in Human Lung Cancer A549 Cells The anticancer activity of Auricularia polytricha polysaccharides towards A549 human lung cancer cells and its underlying mechanisms were investigated. [Int J Biol Macromol] Abstract Nitric Oxide Mediates Cell Aggregation and Mesenchymal to Epithelial Transition in Anoikis-Resistant Lung Cancer Cells Cancer cell aggregation has been long known to facilitate metastatic potential of cancer cells. In addition, the presence of nitric oxide in cancer area may have a significant impact on aggregation behavior of the cells. Scientists showed that lung cancer H460 cells possessing high ability of anoikis resistance formed loose aggregates in detached condition. [Mol Cell Biochem] Abstract |