Influenza Virus Replication in Lung Epithelial Cells Depends on Redox-Sensitive Pathways Activated by NOX4-Derived ROS Researchers demonstrate that influenza A virus infection of lung epithelial cells causes a significant reactive oxygen species (ROS) increase that depends mainly on NADPH oxidase 4 (NOX4), which is up-regulated at both mRNA and protein levels, while the expression of NOX2, the primary source of ROS in inflammatory cells, is down-regulated. [Cell Microbiol] Abstract Bone Morphogenetic Protein 4 Inhibits Liposaccharide-Induced Inflammation in the Airway Investigators demonstrate that bone morphogenetic protein 4 was upregulated in lungs undergoing liposaccharide-induced inflammation, and in airway epithelial cells treated with liposaccharide or TNF-α. [Eur J Immunol] Abstract cAMP-Dependent Protein Kinase Activation Decreases Cytokine Release in Bronchial Epithelial Cells Scientists hypothesized that a bidirectional mechanism involving cAMP-dependent protein kinase and protein kinase C regulates epithelial airway inflammatory responses. To test this hypothesis, primary human bronchial epithelial cells and BEAS-2B cells were treated with hog dust extract in the presence or absence of cAMP. [Am J Physiol Lung Cell Mol Physiol] Abstract Key Role for Store-Operated Ca2+ Channels in Activating Gene Expression in Human Airway Bronchial Epithelial Cells Investigators evaluated if store-operated Ca2+ channels were expressed in human bronchial epithelial cells and, if so, whether they coupled Ca2+ entry to gene expression. [PLoS One] Full Article Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells Researchers demonstrate methods for isolating induced alveolar epithelial progenitor cells from human embryonic stem cells and induced pluripotent stem cells. [Stem Cell Reports] Full Article | Graphical Abstract LUNG CANCER Transcription Factor PREP1 Induces EMT and Metastasis by Controlling the TGF-β-SMAD3 Pathway in Non-Small Cell Lung Adenocarcinoma Scientists show that, in addition to inhibiting primary tumor growth, pre-B-cell leukemia homeobox-regulating protein-1 (PREP1) is a novel epithelial-mesenchymal transition (EMT) inducer and prometastatic transcription factor. In human non-small cell lung cancer cells, PREP1 overexpression is sufficient to trigger EMT, whereas PREP1 down-regulation inhibits the induction of EMT in response to TGF-β. [Proc Natl Acad Sci USA] Abstract miR-340 Inhibits Tumor Cell Proliferation and Induces Apoptosis by Targeting Multiple Negative Regulators of p27 in Non-Small Cell Lung Cancer Researchers describe miR-340 as a novel tumor suppressor in non-small cell lung cancer. Starting from the observation that the growth-inhibitory and proapoptotic effects of miR-340 correlate with the accumulation of p27 in lung adenocarcinoma and glioblastoma cells, they analyzed the functional relationship between miR-340 and p27 expression. [Oncogene] Abstract Dishevelled-3 Activates p65 to Upregulate p120-Catenin Transcription via a p38-Dependent Pathway in Non-Small Cell Lung Cancer Scientists show that Dishevelled-3 activates a p3→p65→PAX2→p120-catenin pathway to affect biological behavior of non-small cell lung cancer (NSCLC) cells. [Mol Carcinog] Abstract miR-204 Functions as a Tumor Suppressor by Regulating SIX1 in NSCLC Researchers analyzed the expression of miR-204 in tumor- and adjacent-tissue samples from 141 patients with non-small cell lung cancer (NSCLC). SIX1 was identified as a potential target of miR-204, and SIX1 silencing partially compromised the invasive and proliferative capacity of miR-204-deficient cells [FEBS Lett] Abstract Combined Inhibition of Hsp90 and the Proteasome Affects NSCLC Proteostasis and Attenuates Cell Migration Scientists suggest that the therapeutic efficacy of heat shock protein 90 (Hsp90) inhibition may be augmented by coadministering proteasome inhibitor on human non-small-cell lung cancer (NSCLC) cell lines. [Anticancer Drugs] Abstract |