MicroRNA Mimicry Blocks Pulmonary Fibrosis Scientists show that intravenous injection of synthetic RNA duplexes can increase microRNA (miR)-29 levels in vivo for several days. Moreover, therapeutic delivery of these miR-29 mimics during bleomycin-induced pulmonary fibrosis restores endogenous miR-29 function whereby decreasing collagen expression and blocking and reversing pulmonary fibrosis. [EMBO Mol Med] Full Article | Press Release Impaired TLR4 and HIF Expression in Cystic Fibrosis Bronchial Epithelial Cells Downregulates Hemeoxygenase-1 and Alters Iron Homeostasis In Vitro Researchers investigated in vitro the role of toll-like receptor 4 (TLR4), hypoxia-inducible factor (HIF)-1α and iron on hemeoxygenase-1 expression in cystic fibrosis. [Am J Physiol Lung Cell Mol Physiol] Abstract Astaxanthin Inhibits Apoptosis in Alveolar Epithelial Cells Type II In Vivo and In Vitro through the ROS-Dependent Mitochondrial Signaling Pathway Scientists investigated the antioxidative effects of astaxanthin against H2O2-induced mitochondrial dysfunction and reactive oxygen species (ROS) production in alveolar epithelial cells type II in vivo and in vitro. [J Cell Mol Med] Full Article Human MicroRNA-24 Modulates Highly Pathogenic Avian-Origin H5N1 Influenza A Virus Infection in A549 Cells by Targeting Secretory Pathway Furin Researchers report that microRNA (miR)-24 and furin are differentially expressed in human A549 cells infected with highly pathogenic avian-origin influenza A H5N1. Using miR-24 mimics, they demonstrate a robust decrease in both furin mRNA level and intracellular furin activity in A549 cells. [J Gen Virol] Abstract Environmental Particulate (PM2.5) Augments Stiffness-Induced Alveolar Epithelial Cell Mechanoactivation of Transforming Growth Factor Beta Scientists explored the relationship between tissue stiffness and exposure to environmental stimuli in the activation of transforming growth factor (TGF)β. They hypothesized that exposure of alveolar type II (ATII) cells to fine particulate matter (PM2.5) would result in enhanced cell contractility, TGFβ activation, and subsequent changes to ATII cell phenotype. [PLoS One] Full Article miR-135a Inhibition Protects A549 Cells from LPS-Induced Apoptosis by Targeting Bcl-2 Researchers hypothesized that certain microRNAs (miRNAs) may play deleterious or protective roles in acute lung injury via the regulation of Bcl-2. They confirmed that Bcl-2 is involved in lipopolysaccharide (LPS)-induced apoptosis in A549 cells and that miR-135a was markedly increased in LPS-challenged A549 cells. [Biochem Biophys Res Commun] Abstract LUNG CANCER Mycobacterium bovis BCG Promotes Tumor Cell Survival from Tumor Necrosis Factor-α-Induced Apoptosis Scientists explored the possible mechanism of M. bovis Bacillus Calmette-Guérin (BCG)-assisted tumorigenicity in type II epithelial cells, human lung adenocarcinoma A549 and other cancer cells. [Mol Cancer] Full Article Inhibition of the JAK/STAT Pathway with Ruxolitinib Overcomes Cisplatin Resistance in Non-Small-Cell Lung Cancer (NSCLC) Scientists elucidated the roles of inhibition of related JAK/STAT pathways in regulating cytotoxicity induced by cisplatin in NSCLC cells. They treated five NSCLC cell lines with cisplatin alone or with cisplatin and Jak2 inhibitor (ruxolitinib) and assessed cell viability, expression of Jak2 and STAT3 and cell apoptosis. [Apoptosis] Abstract Promotion of p53 Expression and Reactive Oxidative Stress Production Is Involved in Zerumbone-Induced Cisplatin Sensitization of Non-Small Cell Lung Cancer Cells The authors investigated the anticancer effects of zerumbone on non-small cell lung cancer cells and explored the involvement of p53 signaling. [Biochimie] Abstract Thymoquinone Inhibits Proliferation and Invasion of Human Nonsmall-Cell Lung Cancer Cells via ERK Pathway The authors investigated the effects of thymoquinone on cell proliferation, migration, and invasion as well as its underlying anti-metastatic mechanisms in A549 cells. [Tumor Biol] Abstract MicroRNA-217 Functions as a Tumor Suppressor Gene and Correlates with Cell Resistance to Cisplatin in Lung Cancer Researchers evaluated the function of microRNA (miR)-217 in lung cancer and investigate its effect on the sensitivity of lung cancer cells to cisplatin. The effects of miR-217 overexpression on the proliferation, apoptosis, migration and invasion of SPC-A-1 and A549 cells were investigated. [Mol Cells] Abstract | Full Article |