Hyperoxia Inhibits Nitric Oxide Treatment Effects in Alveolar Epithelial Cells via Effects on L-Type Amino Acid Transporter-1 Hyperoxia impaired nitric oxide effects on S-nitrosothiol accumulation and soluble guanylyl cyclase activation in proportion to the magnitude of hyperoxia and the duration of exposure, up to 12 hours, in type I-like and type II-like rat and human alveolar epithelial cells. [Antioxid Redox Signal] Abstract Cathepsin B Contributes to Na+ Hyperabsorption in Cystic Fibrosis Airway Epithelial Cultures In bronchial epithelia from both normal and cystic fibrosis (CF) donor lungs, cathepsin B (CTSB) localized to the apical membrane. In normal and CF human bronchial epithelial cultures, CTSB was detected at the apical plasma membrane and in the airway surface liquid. [J Physiol] Abstract Oxidative Stress Regulates CFTR Gene Expression in Human Airway Epithelial Cells through a Distal Antioxidant Response Element Cystic fibrosis transmembrane conductance regulator gene (CFTR) expression in human airway epithelial cells involves the recruitment of distal cis-regulatory elements, which are associated with airway-selective DNase hypersensitive sites at -44kb and -35kb from the gene. Scientists investigated the -44 kb element, which also has enhancer activity in vitro in airway epithelial cells but is inactive in intestinal epithelial cells. [Am J Respir Cell Mol Biol] Abstract Phospholipase Cε, an Effector of Ras and Rap Small GTPases, Is Required for Airway Inflammatory Response in a Mouse Model of Bronchial Asthma Primary-cultured bronchial epithelial cells prepared from phospholipase Cε (PLCε)ΔX/ΔX mice showed attenuated pro-inflammatory cytokine production when stimulated with tumor necrosis factor-α, suggesting that reduced cytokine production in PLCεΔX/ΔX mice was due to cell-autonomous effect of PLCε deficiency. [PLoS One] Full Article Insulin Upregulates the Expression of Epithelial Sodium Channel In Vitro and in a Mouse Model of Acute Lung Injury: Role of mTORC2/SGK1 Pathway In alveolar epithelial cells, insulin increased the expression of α-, β-, and γ-epithelial sodium channel, which was blocked by the mammalian target of rapamycin complex 2 (mTORC2) inhibitor or knockdown of Rictor, and serum and glucocorticoid induced kinase-1 (SGK1) inhibitor, respectively. [Exp Cell Res] Abstract Stanniocalcin-1 Is Induced by Hypoxia Inducible Factor in Rat Alveolar Epithelial Cells Scientists found that stanniocalcin-1 (STC1) was induced by hypoxia and hypoxia inducible factor in rat alveolar type II cells, and this induction occurred rapidly and reversibly. They also show that recombinant human STC1 enhanced cell motility with extended lamellipodia formation in alveolar epithelial cell monolayers but did not inhibit the oxidative damage induced by LPS. [Biochem Biophys Res Commun] Abstract LUNG CANCER Tissue Inhibitor of Metalloproteinases-1 Induces a Pro-Tumorigenic Increase of miR-210 in Lung Adenocarcinoma Cells and Their Exosomes Researchers demonstrate that an increase of both exogenous and endogenous tissue inhibitor of metalloproteinases-1 led to the upregulation of miR-210 in a CD63/PI3K/AKT/HIF-1-dependent pathway in lung adenocarcinoma cells. [Oncogene] Abstract RET Mutation and Expression in Small-Cell Lung Cancer Stable overexpression of both mutant M918T and wild-type RET in two small-cell lung cancer cell lines, H1048 and SW1271, activated ERK signaling, MYC expression, and increased cell proliferation, particularly by mutant RET. [J Thorac Oncol] Full Article miR-630 Inhibits Proliferation by Targeting CDC7 Kinase, but Maintains the Apoptotic Balance by Targeting Multiple Modulators in Human Lung Cancer A549 Cells Scientists found that microRNA-630 (miR-630) was highly expressed in A549 and NIH3T3 cells where cell-cycle kinase 7 (CDC7) was downregulated, but lower in H1299, MCF7, MDA-MB-231, HeLa and 2BS cells where CDC7 was upregulated. The induction of miR-630 occurred commonly in a variety of human cancer and immortalized cells in response to genotoxic agents. [Cell Death Dis] Full Article MicroRNA-191, by Promoting the EMT and Increasing CSC-Like Properties, Is Involved in Neoplastic and Metastatic Properties of Transformed Human Bronchial Epithelial Cells High-throughput microarray analysis showed that 51 microRNAs were differentially expressed in transformed human bronchial epithelial (HBE) cells relative to normal HBE cells. In particular, microRNA-191 was up-regulated in transformed cells. [Mol Carcinog] Abstract |