miRNA-30a Inhibits AECs-II Apoptosis by Blocking Mitochondrial Fission Dependent on Drp-1 Researchers investigated whether miR-30a could block type II alveolar epithelial cells (AECs-II) apoptosis by repressing mitochondrial fission dependent on dynamin-related protein-1 (Drp-1). [J Cell Mol Med] Abstract | Full Article Extracellular Signal-Regulated Kinase Pathway Play Distinct Role in Acetochlor-Mediated Toxicity and Intrinsic Apoptosis in A549 Cells Researchers elucidated the effect and molecular mechanisms of acetochlor (ACETO) on human alveolar A549 cells. Established assays of cell viability and cytotoxicity were performed to detect the potential effects of ACETO on A549 cells. [Toxicol In Vitro] Abstract Non-Specific Interaction of Carbon Nanotubes with the Resazurin Assay Reagent: Impact on In Vitro Assessment of Nanoparticle Cytotoxicity Nanomaterials (NMs), in particular carbon nanotubes can interfere in assays through quenching of transmitted light or fluorescence. Scientists show that using the resazurin assay with time-point reading of clarified supernatants resolves this problem. Human lung epithelial and murine macrophage cell lines were exposed to NMs in 96-well plates in 200 μL of media/well. [Toxicol In Vitro] Full Article Systematic Identification of Transcriptional and Post-Transcriptional Regulations in Human Respiratory Epithelial Cells during Influenza A Virus Infection The molecular mechanisms of airway epithelial cell responses to viral infection are not fully understood. Researchers propose a novel framework named SITPR to investigate the interactions among transcription factors, microRNAs and target genes. [BMC Bioinformatics] Abstract | Full Article LUNG CANCER Differential Roles of STAT3 in the Initiation and Growth of Lung Cancer Scientists demonstrate that STAT3 has contrasting roles in the initiation and growth of both chemically and genetically induced lung cancers. Deletion of lung epithelial STAT3 after establishment of lung cancer inhibited cancer cell proliferation. [Oncogene] Abstract Reduced miR-3127-5p Expression Promotes NSCLC Proliferation/Invasion and Contributes to Dasatinib Sensitivity via the c-Abl/Ras/ERK Pathway Overexpression of miR-3127-5p significantly reduced non-small-cell lung cancer (NSCLC) cells proliferation, migration, and motility in vitro and in vivo. [Sci Rep] Full Article The Expression of p21 Is Upregulated by Forkhead Box A1/2 in p53-Null H1299 Cells Scientists demonstrate that forkhead box A1/2 is a crucial transcription factor in the activation of p21 transcription via direct binding to the p21 promoter in p53-null H1299 lung carcinoma cells. [FEBS Lett] Abstract A Flavonoid Isolated from Streptomyces sp. (ERINLG-4) Induces Apoptosis in Human Lung Cancer A549 Cells through p53 and Cytochrome C Release Caspase Dependent Pathway Cytotoxic properties of ethyl acetate extract was carried out against the A549 lung cancer cell line using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cytotoxic properties of isolated compound were investigated in the A549 lung cancer cell line, COLO320DM cancer cell line and Vero cells. [Chem Biol Interact] Abstract Expression of MicroRNA-96 and Its Potential Functions by Targeting FOXO3 in Non-Small Cell Lung Cancer Quantitative reverse transcription PCR was performed to identify the microRNA (miR)-96 expression level in 68 paired non-small cell lung cancer and adjacent normal lung tissues. Trans-well, cell counting kit-8, and apoptosis assays were used to evaluate the effects of miR-96 expression on cell invasion, proliferation, and apoptosis. [Tumor Biol] Abstract |