Pulmonary Cell News Volume 3.47 | Dec 4 2014

Effects in Cigarette Smoke Stimulated Bronchial Epithelial Cells of a Corticosteroid Entrapped into Nanostructured Lipid Carriers
Nanostructured lipid carriers (NLC) containing Fluticasone propionate were prepared and their biocompatibility and effects in a human bronchial epithelial cell line stimulated with cigarette smoke extracts were tested. Biocompatibility studies showed that the NLC did not induce cell necrosis or apoptosis. [J Nanobiotechnology] Abstract | Full Article

Genotoxic and Epigenotoxic Effects of Fine Particulate Matter from Rural and Urban Sites in Lebanon on Human Bronchial Epithelial Cells
Using two different particulate matter (PM)_2.5-0.3 samples collected at an urban and a rural site, scientists examined genotoxic/epigenotoxic effects of PM exposure within a human bronchial epithelial cell line. [Environ Res] Abstract

SILAC-Based Quantitative Proteomic Analysis of Human Lung Cell Response to Copper Oxide Nanoparticles
Researchers evaluated the response of BEAS-2B human lung cells to Copper (II) oxide (CuO) nanoparticles (NP), using stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics and phosphoproteomics. Pathway modeling of the protein differential expression showed that CuO NP affect proteins relevant in cellular function and maintenance, protein synthesis, cell death and survival, cell cycle and cell morphology. [PLoS One] Full Article

Delay of Airway Epithelial Wound Repair in COPD Is Associated with Airflow Obstruction Severity
Researchers determined if airway epithelial wound repair of airway epithelium is abnormal in chronic obstructive pulmonary disease (COPD). They determined the mean speed of wound closure and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of bronchial epithelial cells 18 hours after cell wounding. [Respir Res] Full Article

Toxicity of Nanoparticles Embedded in Paints Compared to Pristine Nanoparticles, In Vitro Study
The in vitro toxicity of three pristine ENPs, three aged paints containing ENPs and control paints without ENPs were compared. Cytotoxicity was assessed using a biculture consisting of human bronchial epithelial cells and human monocytic cells to determine subtoxic concentrations. [Toxicol Lett] Abstract

LUNG CANCER

Downregulation of miR-25 Modulates Non-Small Cell Lung Cancer Cells by Targeting CDC42
Researchers investigated the functional role of miRNA-25 in non-small cell lung cancer (NSCLC) cells. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-25 in NSCLC cell lines and 11 pairs of human NSCLC and non-cancerous tissues. [Tumor Biol] Abstract

Excision Repair Cross Complementation Group 1 Is a Chemotherapy-Tolerating Gene in Cisplatin-Based Treatment for Non-Small Cell Lung Cancer
Researchers evaluated the biological functions of excision repair cross complementation group 1 in cell proliferation, cell cycle, invasion and cisplatin response of non-small cell lung cancer cells. [Int J Oncol] Abstract

MicroRNA-449a Suppresses Non-Small Cell Lung Cancer
Scientists transfected microRNA-449a mimic and inhibitor in lung cancer cell line A549, and used western blot to determine the expression level of apoptosis-related molecules Bcl-2 and p53. [Cell Biochem Biophys] Abstract

Maslinic Acid Induces Mitochondrial Apoptosis and Suppresses HIF-1α Expression in A549 Lung Cancer Cells under Normoxic and Hypoxic Conditions
The apoptotic effects of maslinic acid (MA) at 4, 8, 16, 32 and 64 μmol/L on human lung cancer A549 cells under normoxic and hypoxic conditions were examined. MA at 4-64 and 16-64 μmol/L lowered Bcl-2 expression under normoxic and hypoxic conditions, respectively. [Molecules] Abstract | Download Full Article

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FDA Approves PulmoFlow’s Tobramycin & Nebulizer Combination for Cystic Fibrosis
The FDA granted final approval of PulmoFlow, Inc.’s New Drug Application for Kitabis Pak – a co-packaging of generic tobramycin inhalation solution with a PARI LC PLUS^® Nebulizer. This is the first nebulized drug and device combination to be approved for patients with cystic fibrosis. [PARI International] Press Release

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