Pulmonary Cell News Volume 4.04 | Feb 5 2015

Efficient Generation of Functional ​CFTR-Expressing Airway Epithelial Cells from Human Pluripotent Stem Cells
This protocol describes how to generate ​cystic fibrosis transmembrane conductance regulator protein (​CFTR)-expressing airway epithelial cells from human pluripotent stem cells. [Nat Protoc] Abstract

House Dust Mite Allergens Induce Monocyte IL-1β Production Triggering an IκBζ Dependent GMCSF Release from Human Lung Epithelial Cells
Scientists analyzed IκBζ‘s role in regulating lung epithelial cell cytokine responses to house dust mite mix (HDM). They found that human bronchial epithelial cells express IκBζ, and release IL-6 and GMCSF when co-cultured with human monocytes and HDM. [Am J Respir Cell Mol Biol] Abstract

Mechanism of Dose-Dependent Regulation of UBE1L by Polyphenols in Human Bronchial Epithelial Cells
The effect of several chemopreventive polyphenols on ubiquitin activating enzyme E1-like (UBE1L) expression in human bronchial epithelial cells was investigated. Lower concentrations of curcumin, (-)-epigallocatechin gallate (EGCG) and resveratrol upregulated UBE1L, while high concentrations of curcumin, EGCG and resveratrol downregulated UBE1L levels. [J Cell Biochem] Abstract

LUNG CANCER

Radiation Promotes Invasiveness of Non-Small-Cell Lung Cancer Cells through Granulocyte-Colony-Stimulating Factor
Radiation-induced granulocyte-colony-stimulating factor promoted migratory and invasive properties by triggering the epithelial-mesenchymal cell transition in non-small-cell lung cancer cells. [Oncogene] Abstract

Simvastatin Downregulates Expression of TGF-βRII and Inhibits Proliferation of A549 Cells via ERK
Researchers demonstrated that simvastatin inhibited activation of ERK, downregulated expression of transforming growth factor-β receptor II (TGF-βRII), and suppressed A549 cell proliferation. [Tumor Biol] Abstract

Maclurin Suppresses Migration and Invasion of Human Non-Small-Cell Lung Cancer Cells via Anti-Oxidative Activity and Inhibition of the Src/FAK-ERK-β-Catenin Pathway
Scientists report that maclurin, a major phenolic component of ethanol extracted mulberry twigs, exerts anti-metastatic effect in A549 human non-small-cell lung cancer cells. [Mol Cell Biochem] Abstract

AURKA Upregulation Plays a Role in Fibroblast-Reduced Gefitinib Sensitivity in the NSCLC Cell Line HCC827
Researchers investigated the role of fibroblasts in stromal cell-mediated resistance to gefitinib-induced apoptosis in epidermal growth factor receptor (EGFR)-mutant non-small cell lung carcinoma (NSCLC) cells. While gefitinib induced apoptosis in EGFR-mutant NSCLC cells, apoptosis induction was diminished under stromal co-culture conditions. [Oncol Rep] Abstract

Growth Inhibitory Effects of miR-221 and miR-222 in Non-Small Cell Lung Cancer Cells
Scientists investigated the effects of miR-221 and miR-222 overexpression on six lung cancer cell lines, including H460, as well as one immortalized normal human bronchial epithelial cell line, HBEC4. miR-221 and miR-222 induced epithelial-to-mesenchymal transition-like changes in a minority of HBEC4 cells but, unexpectedly, both the microRNAs rather suppressed their invasiveness. [Cancer Med] Full Article

Visit our reviews page to see a complete list of reviews in the pulmonary cell research field.

Theravance Biopharma, Inc. and Mylan Partner to Develop and Commercialize a Novel LAMA Compound, TD-4208, for COPD
Theravance Biopharma, Inc. and Mylan Inc. announced that the two companies will partner on the development and, subject to FDA approval, commercialization of TD-4208, a novel investigational once-daily nebulized long-acting muscarinic antagonist (LAMA) for chronic obstructive pulmonary disease (COPD) and other respiratory diseases. [Theravance Biopharma, Inc.] Press Release

Celtaxsys Lead Candidate Granted Orphan Designation in Cystic Fibrosis by FDA
Celtaxsys, Inc. announced that the U.S. Food and Drug Administration (FDA) has granted orphan drug designation to its once daily, oral leukotriene A4 hydrolase inhibitor for the treatment of cystic fibrosis. [Celtaxsys, Inc.] Press Release

Ignyta Receives Orphan Drug Designation from FDA for Entrectinib for the Treatment of Molecularly Defined Subsets of Non-Small Cell Lung Cancer
Ignyta, Inc. announced that the U.S. Food and Drug Administration (FDA) has granted orphan drug designation for Ignyta’s lead product candidate entrectinib for the treatment of TrkA-positive, TrkB-positive, TrkC-positive, ROS1-positive and ALK-positive non-small cell lung cancer. [Ignyta, Inc.] Press Release

FDA Grants Breakthrough Therapy Designation for Genentech’s Investigational Cancer Immunotherapy MPDL3280A (Anti-PDL1) in Non-Small Cell Lung Cancer
Genentech announced that it has received a second breakthrough therapy Ddesignation from the U.S. Food and Drug Administration (FDA) for its investigational cancer immunotherapy MPDL3280A (anti-PDL1). [Genentech, Inc.] Press Release

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NEW Senior Process Development Engineer (Sangamo BioSciences, Inc.)

NEW PhD Studentships – Molecular Imaging and Sensing of Lung Inflammation, Infection and Regeneration (University of Edinburgh)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Postdoctoral Fellow – Lung Cancer Development and Progression (The University of Texas MD Anderson Cancer Center)

Postdoctoral Fellow – Lung Cancer (MD Anderson Cancer Center)

Postdoctoral Fellow – Cystic Fibrosis Research (University of Tennessee Health Science Center)

Postdoctoral Research Fellow – Post-Transcriptional Gene Regulation Governing Cancer Cell Behavior (Fred Hutchinson Cancer Research Center)

Postdoctoral Fellow – Wnt Signaling in Development and Disease (Van Andel Research Institute)

PhD Students – Heart and Lung Research (Max Planck Institute for Heart and Lung Research)

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