Pulmonary Cell News Volume 4.05 | Feb 12 2015

High Glucose Promotes Tumor Invasion and Increases Metastasis-Associated Protein Expression in Human Lung Epithelial Cells by Upregulating Heme Oxygenase-1 via Reactive Oxygen Species or the TGF-β₁/PI3K/Akt Signaling Pathway
Researchers investigated the promotion of tumor cell invasion and the expression of metastasis-associated proteins by inducing the up-regulation of heme oxygenase-1 expression by high glucose treatment in A549 human lung epithelial cells. [Cell Physiol Biochem] Full Article

Plasticity of Airway Epithelial Cell Transcriptome in Response to Flagellin
Researchers compared the transcriptional effects of flagellin on airway epithelial cells (AECs) in monolayer culture versus air-liquid interface (ALI) culture using whole-genome microarrays and RNA sequencing. They exposed monolayer and ALI AEC cultures to flagellin in vitro and analyzed the transcriptional response by microarray and RNA-sequencing. [PLoS One] Full Article

Oxidative Stress, Apoptosis, and Cell Cycle Arrest Are Induced in Primary Fetal Alveolar Type II Epithelial Cells Exposed to Fine Particulate Matter from Cooking Oil Fumes
Oxidative stress, cytotoxicity, apoptosis, and cell cycle arrest induced by cooking oil fume-derived particularly fine particulate matter in primary fetal alveolar type II epithelial cells were detected. [Environ Sci Pollut Res Int] Abstract

Apigenin Inhibits PMA-Induced Expression of Pro-Inflammatory Cytokines and AP-1 Factors in A549 Cells
Scientists investigated the pro-inflammatory cytokines and pattern of AP-1 factors expressed during activation of lung adenocarcinoma A549 cells by phorbol-12-myristate-13-acetate (PMA) and aimed to understand the anti-inflammatory effect of apigenin (AP-1). [Mol Cell Biochem] Abstract

LUNG CANCER

Lysyl Hydroxylase 2 Induces a Collagen Cross-Link Switch in Tumor Stroma
Utilizing resected human lung cancer tissues and a p21^CIP1/WAF1-deficient, K-ras^G12D-expressing murine metastatic lung cancer model, scientists showed that, relative to normal lung tissues, tumor stroma contains higher levels of hydroxylysine aldehyde-derived collagen cross-links and lower levels of lysine aldehyde-derived cross-links. [J Clin Invest] Full Article | Press Release

In Vitro Cultured Lung Cancer Cells Are Not Suitable for Animal-Based Breath Biomarker Detection
In vitro cultured lung cancer cell lines were investigated regarding the possible identification of volatile organic compounds as potential biomarkers. Gas samples from the headspace of pure culture medium and from the cultures of human lung adenocarcinoma cell lines A549 and Lu7466 were exposed to polypropylene fleece in order to absorb odour components. [J Breath Res] Abstract

Down-Regulation of ERK1/2 and AKT-Mediated X-Ray Repair Cross-Complement Group 1 Protein (XRCC1) Expression by Hsp90 Inhibition Enhances the Gefitinib-Induced Cytotoxicity in Human Lung Cancer Cells
Gefitinib treatment decreased XRCC1 mRNA and protein expression through extracellular signal-regulated kinases 1/2 (ERK1/2) and AKT inactivation in two non-small cell lung cancer cells, A549 and H1975. Knocking down XRCC1 expression by transfection with small interfering RNA of XRCC1 enhanced the cytotoxicity and cell growth inhibition of gefitinib. [Exp Cell Res] Abstract

miR-342-3p Targets RAP2B to Suppress Proliferation and Invasion of Non-Small Cell Lung Cancer Cells
Scientists showed that miR-342-3p is downregulated in non-small cell lung cancer (NSCLC) cell lines and tissues, and its overexpression induced significant inhibition of NSCLC cell proliferation, invasion, and tumor growth in nude mice. [Tumor Biol] Abstract

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Advanced Inhalation Therapies Receives Orphan Drug Designation of Its Proprietary High Dose Formulation of Nitric Oxide for Adjunctive Treatment of Cystic Fibrosis
Advanced Inhalation Therapies Ltd. announced that the U.S. Food and Drug Administration (FDA) granted orphan drug designation to AIT-CF, the company’s proprietary high dose formulation of nitric oxide for adjunctive treatment of cystic fibrosis. [Advanced Inhalation Therapies Ltd.] Press Release

University of Maryland Medicine Announces New National Program in Lung Healing to Develop Innovative Approaches for Preventing and Treating Acute Lung Disease
University of Maryland School of Medicine announced the official launch of a new “Program in Lung Healing,” that will further the school’s position as a national leader in research, education and clinical innovation for acute ailments of the lung and respiratory system. [University of Maryland School of Medicine] Press Release

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Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Media Development, High Throughput Screening (STEMCELL Technologies Inc.)

Scientist – Cell Culture Support Products (STEMCELL Technologies Inc.)

Director – Vector Production (Sangamo BioSciences, Inc.)

Senior Process Development Engineer (Sangamo BioSciences, Inc.)

PhD Studentships – Molecular Imaging and Sensing of Lung Inflammation, Infection and Regeneration (University of Edinburgh)

Postdoctoral Fellow – Lung Cancer Development and Progression (The University of Texas MD Anderson Cancer Center)

Postdoctoral Fellow – Lung Cancer (MD Anderson Cancer Center)

Postdoctoral Fellow – Cystic Fibrosis Research (University of Tennessee Health Science Center)

Postdoctoral Research Fellow – Post-Transcriptional Gene Regulation Governing Cancer Cell Behavior (Fred Hutchinson Cancer Research Center)

Postdoctoral Fellow – Wnt Signaling in Development and Disease (Van Andel Research Institute)

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