PARK2-Mediated Mitophagy Is Involved in Regulation of HBEC Senescence in COPD Pathogenesis Researchers sought to investigate to determine if PTEN-induced putative kinase 1-PARK2-mediated mitophagy is involved in the regulation of cigarette smoke extract-induced cell senescence and in COPD pathogenesis. Mitochondrial damage, reactive oxygen species production, and cell senescence were evaluated in primary human bronchial epithelial cells. [Autophagy] Abstract Pseudomonas aeruginosa-Induced Bleb-Niche Formation in Epithelial Cells Is Independent of Actinomyosin Contraction and Enhanced by Loss of Cystic Fibrosis Transmembrane-Conductance Regulator Osmoregulatory Function Scientists examined mechanisms for P. aeruginosa-induced bleb formation, focusing on its relationship to apoptosis and the cystic fibrosis transmembrane-conductance regulator (CFTR). Cells with defective CFTR displayed enhanced bleb formation upon infection, as demonstrated using bronchial epithelial cells from a patient with cystic fibrosis and a CFTR inhibitor, CFTR(Inh)-172. [MBio] Full Article Involvement of Urokinase in Cigarette Smoke Extract-Induced Epithelial-Mesenchymal Transition in Human Small Airway Epithelial Cells Researchers studied the urokinase-type plasminogen activator (uPA) regulation in cigarette smoke extract (CSE)-induced epithelial-mesenchymal transition in human small airway epithelial cell lines. uPA was overexpressed in the small airway epithelium of chronic obstructive pulmonary disease patients and CSE-treated cell lines. [Lab Invest] Abstract Direct Contact between Dendritic Cells and Bronchial Epithelial Cells Inhibits T Cell Recall Responses towards Mite and Pollen Allergen Extracts In Vitro Using an in vitro co-culture model of the airway epithelium, where a polarized cell layer of bronchial epithelial cells can interact with dendritic cells, scientists investigated recall T cell responses in allergic patients sensitized to house dust mite, grass, and birch pollen. [Clin Exp Immunol] Abstract LUNG CANCER Combinatorial Action of MicroRNAs let-7 and miR-34 Effectively Synergizes with Erlotinib to Suppress Non-Small Cell Lung Cancer Cell Proliferation Scientists examined the ability of two tumor suppressor microRNAs (miRNAs), let-7b and miR-34a to sensitize KRAS;TP53 mutant non-small cell lung cancer cells to the action of erlotinib. Treatment with these miRNAs, individually or in combination, resulted in synergistic potentiation of the anti-proliferative effects of erlotinib. [Cell Cycle] Abstract Cyclic AMP Signaling Reduces Sirtuin 6 Expression in Non-Small Cell Lung Cancer Cells by Promoting Ubiquitin-Proteasomal Degradation via Inhibition of the Raf-MEK-ERK Pathway Researchers examined the effects of cAMP signaling on sirtuin 6 expression and radiation-induced apoptosis in lung cancer cells. cAMP signaling in H1299 and A549 human non-small cell lung cancer cells was activated via the expression of constitutively active Gαs plus treatment with prostaglandin E2, isoproterenol, or forskolin. [J Biol Chem] Abstract | Full Article Trametinib with or without Vemurafenib in BRAF Mutated Non-Small Cell Lung Cancer Investigators found that the combination of vemurafenib and erlotinib was not synergistic to the inhibition of p-ERK signaling in BRAF-V600E cells. Vemurafenib caused significant apoptosis, G1 arrest and upregulation of BIM in BRAF-V600 cells. [PLoS One] Full Article Anticancer Effects of Adenovirus-Mediated Calreticulin and Melanoma-Associated Antigen 3 Expression on Non-Small Cell Lung Cancer Cells Researchers examined the effect of adenovirus-mediated melanoma-associated antigen 3 and/or calreticulin expression on the proliferation, invasion, and apoptosis of human non-small cell lung cancer cells and on the vascular tube formation of human endothelial cells as well as on dendritic cell activation and induced CD8+ cytotoxic T lymphocyte activity in vitro. [Int Immunopharmacol] Abstract |