Pulmonary Cell News Volume 4.14 | Apr 16 2015

Human Normal Bronchial Epithelial Cells: A Novel In Vitro Cell Model for Toxicity Evaluation
Scientists established human normal bronchial epithelial cells using a defined primary epithelial cell culture medium without transduction of exogenous genes. [PLoS One] Full Article

MAPK Pathway Mediates Epithelial-Mesenchymal Transition Induced by Paraquat in Alveolar Epithelial Cells
Investigators showed that paraquat-induced epithelial RLE-6NT cells developed mesenchymal cell characteristics, as indicated by a significant decrease in the epithelial marker E-cadherin and a significant increase in the extracellular matrix marker α-smooth muscle actin in a dose and time-dependent manner. [Environ Toxicol] Abstract

Evaluation of Gene Expression Changes in Human Primary Lung Epithelial Cells Following 24-Hr Exposures to Inorganic Arsenic and Its Methylated Metabolites and to Arsenic Trioxide
The concentration response for altered gene expression in primary lung epithelial cells was determined following two treatments with arsenicals: a mixture of trivalent arsenic compounds representative of urinary arsenic concentrations in exposed human populations, and arsenite a common form of inhaled arsenic dust that is frequently used in both in vivo and in vitro experimental exposures. [Environ Mol Mutagen] Abstract

HMGB1 Binding to Receptor for Advanced Glycation End Products Enhances Inflammatory Responses of Human Bronchial Epithelial Cells by Activating p38 MAPK and ERK1/2
Researchers investigated receptor expression and proinflammatory cytokine production by primary cultures of human bronchial epithelial cells stimulated by high mobility group box protein 1 (HMGB1). [Mol Cell Biochem] Abstract

LUNG CANCER

Collateral Chemoresistance to Anti-Microtubule Agents in a Lung Cancer Cell Line with Acquired Resistance to Erlotinib
Scientists investigated the sensitivity of erlotinib-resistant tumor cells to five cytotoxic agents using an in vitro epidermal growth factor receptor-tyrosine kinase inhibitor-resistant model. Four erlotinib-sensitive lung adenocarcinoma cell lines and their resistant derivatives were tested. [PLoS One] Full Article

Fructus phyllanthi Tannin Fraction Induces Apoptosis and Inhibits Migration and Invasion of Human Lung Squamous Carcinoma Cells In Vitro via MAPK/MMP Pathways
The authors investigated the anticancer mechanisms of F. phyllanthi tannin fraction in human lung squamous carcinoma cells in vitro. [Acta Pharmacol Sin] Abstract

EGFR-Independent Autophagy Induction with Gefitinib and Enhancement of Its Cytotoxic Effect by Targeting Autophagy with Clarithromycin in Non-Small Cell Lung Cancer Cells
Gefitinib, an inhibitor for EGFR tyrosine kinase, potently induced autophagy in non-small cell lung cancer cell lines such as PC-9 cells expressing constitutively activated EGFR kinase by EGFR gene mutation as well as A549 and H226 cells with wild-type EGFR. [Biochem Biophys Res Commun] Abstract

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DiscoveryBioMed, Inc. Is Developing a Novel “Dual Action” Small Molecule Monotherapy for All Cystic Fibrosis (CF) Patients
DiscoveryBioMed, Inc. has discovered and is developing a novel class of small molecule monotherapy that affects both major drug targets that are abnormal and underlie the pathophysiological features of CF. Proper function of the two target proteins that serve as transporters for salt and water into the airways is critical for hydration of the lung and airways and clearance of mucus and harmful inhaled materials. [DiscoveryBioMed, Inc. (Business Wire)] Press Release

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

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Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Professorship – Lung Epithelial Cell Plasticity (Ludwig-Maximilians-Universitat München)

PhD Position – Development of a 3D In Vitro Model of Infected Airway (Helmholtz Centre for Infection Research)

Research Assistant – Inflammation in the Lung during Viral Infection (Duke-NUS Graduate Medical School Singapore)

PhD Studentship – Cystic Fibrosis (University of East Anglia)

Postdoctoral Fellowship – Pulmonary Genetic Medicine (Cornell University)

Postdoctoral Researcher – Pulmonary Developmental Pathology (Drexel University College of Medicine)

Postdoctoral Position – Airway Disease and Inflammation (Brigham and Women’s Hospital Harvard Medical School)

Postdoctoral Scholar – Molecular Mechanisms of Lung Cancer, Lung Regeneration and Lung Development (University of California, San Francisco)

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