Continuous Mucociliary Transport by Primary Human Airway Epithelial Cells In Vitro Scientists characterized a novel method that allows human airway epithelial cells to differentiate into a mucociliary epithelium that transports mucus in a continuous circular track. [Am J Physiol Lung Cell Mol Physiol] Abstract 14,15-Epoxyeicosatrienoic Acid Suppresses Cigarette Smoke Extract-Induced Apoptosis in Lung Epithelial Cells by Inhibiting Endoplasmic Reticulum Stress Investigators explored the effects of CYP2J2-epoxyeicosatrienoic acids on cigarette smoke extracts-induced apoptosis in human bronchial epithelial cell line and the possible mechanisms involved. [Cell Physiol Biochem] Full Article Induction and Repair of DNA Cross-Links Induced by Sulfur Mustard in the A-549 Cell Line Followed by a Comet Assay Using the A-549 lung cell line, the comet assay protocol was optimized for indirect detection of DNA cross-links induced by sulfur mustard. The method is based on the additional treatment of the assayed cells containing cross-links with the chemical mutagen, styrene oxide. [Chem Biol Interact] Abstract SIRT5 Prevents Cigarette Smoke Extract-Induced Apoptosis in Lung Epithelial Cells via Deacetylation of FOXO3 Researchers showed that cigarette smoke extract (CSE) induces SIRT5 to deacetylate forkhead box O3 (FOXO3) at K271 and K290. Deacetylation of FOXO3 promotes its nuclear localization. Notably, transfection with FOXO3 K271R- or K290R-attenuated CSE-induced apoptosis in SIRT5 knocked down cells, suggesting the protective effects of SIRT5, is mediated by FOXO3. [Cell Stress Chaperones] Abstract In Vitro Effects Induced by Diesel Exhaust at an Air-Liquid Interface in a Human Lung Alveolar Carcinoma Cell Line A549 Researchers examined the effects induced in vitro in human adenocarcinoma-derived alveolar basal epithelial A549 cells by diesel particulate matter administered into the culture medium or by diesel exhaust administered at an air-liquid interface. [Exp Toxicol Pathol] Abstract LUNG CANCER MALT1 Is Required for EGFR-Induced NF-κB Activation and Contributes to EGFR-Driven Lung Cancer Progression The authors found that mucosa-associated lymphoid tissue 1 (MALT1) is involved in epidermal growth factor receptor (EGFR)-induced nuclear factor kappa B (NF-κB) activation in cancer cells, and that MALT1 deficiency impaired EGFR-induced NF-κB activation. MALT1 mainly functions as a scaffold protein by recruiting E3 ligase TRAF6 to IKK complex to activate NF-κB in response to EGF stimulation. [Oncogene] Abstract The New InsP3Kinase Inhibitor BIP-4 Is Competitive to InsP3 and Blocks Proliferation and Adhesion of Lung Cancer Cells Determination of cellular uptake in lung cancer cells revealed that 6% of extracellular BIP-4 is internalized by non-endosomal uptake, showing that BIP-4 is not trapped inside endo/lysosomes but is available to inhibit cellular InsP3Kinase activity. [Biochem Pharmacol] Abstract The Long Noncoding RNA MEG3 Contributes to Cisplatin Resistance of Human Lung Adenocarcinoma Scientists observed the expression of maternally expressed gene 3 (MEG3) in lung adenocarcinoma (LAD) and evaluated its biological role and clinical significance in the resistance of LAD cells to cisplatin. [PLoS One] Full Article MicroRNA miR-10b Inhibition Reduces Cell Proliferation and Promotes Apoptosis in Non-Small Cell Lung Cancer (NSCLC) Cells To study the potential function of miR-10b involved in the regulation of lung tumors, researchers monitored NSCLC cell behavior including proliferation, apoptosis and cell cycle using CCK-8 and flow cytometry analysis. [Mol Biosyst] Abstract miR-183 Promotes Growth of Non-Small Cell Lung Cancer Cells through FoxO1 Inhibition The authors analyzed the effects of microRNA (miR)-183 on non-small cell lung cancer cell growth in vitro using a microculture tetrazolium assay and in vivo by visualizing tumor growth using bioluminescence assay. [Tumor Biol] Abstract |