Genetic Inhibition of the Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated to F508del Cystic Fibrosis Mutation Researchers identified RNF5 as a protein whose inhibition promoted significant deletion of phenylalanine 508 (F508del)-CFTR rescue and displayed an additive effect with the investigational drug VX-809. In addition, they found that RNF5 is differentially expressed in human bronchial epithelia from cystic fibrosis vs. control patients. [Sci Rep] Full Article Analysis of the TGFβ-Induced Program in Primary Airway Epithelial Cells Shows Essential Role of NF-κB/RelA Signaling Network in Type II Epithelial Mesenchymal Transition Researchers applied next generation sequencing in primary human airway epithelial cells to understand the gene program controlling type II epithelial mesenchymal transition and how cytokine-induced inflammation modifies it. [BMC Genomics] Full Article The Kinetic Response of the Proteome in A549 Cells Exposed to ZnSO4 Stress The authors investigated the time-dependent differential expression of the proteome in A549 cells after administered with ZnSO4 for both 9 and 24 hours using 2DE. 123 differentially expressed protein spots were detected, most of which were up-regulated by Zn2+ treatment. [PLoS One] Full Article Maresin1 Inhibits Epithelial-to-Mesenchymal Transition In Vitro and Attenuates Bleomycin Induced Lung Fibrosis In Vivo Scientists investigated whether MaR1, a kind of pro-resolving lipid mediators, could inhibit TGF-β1 induced epithelial to mesenchymal transition in vitro and lung fibrosis in vivo. In in vitro studies, mouse type II alveolar epithelial cells were treated with different doses of MaR1 for 30 minutes and were exposed to TGF-β1 for 48 hours. [Shock] Abstract Cigarette Smoke Affects IL-17A, IL-17F and IL-17 Receptor Expression in the Lung Tissue: Ex Vivo and In Vitro Studies In vitro, human epithelial cell line 16HBE and A549 as well as PBMC from normal donors were stimulated with cigarette smoke extract to evaluate the IL-17A, IL-17F and IL-17R expression by flow cytometry. [Cytokine] Abstract LUNG CANCER EGCG Reverses Human Neutrophil Elastase-Induced Migration in A549 Cells by Directly Binding to HNE and By Regulating α1-AT Investigators demonstrated a novel mechanism by which EGCG reverses the neutrophil elastase-induced migration of A549 cells. [Sci Rep] Full Article Synergistic Effects between mTOR Complex 1/2 and Glycolysis Inhibitors in Non-Small-Cell Lung Carcinoma Cells By employing automated spectrophotometry, scientists found the level of glucose uptake was decreased in non-small-cell lung carcinoma (NSCLC) A549, PC-9 and SK-MES-1 cells treated with rapamycin or siRNA against Raptor, indicating that the inhibition of mammalian target of rapamycin (mTOR) complex 1 attenuated glycolytic metabolism in NSCLC cells. [PLoS One] Full Article Resveratrol Analogue 3,4,4′-Trihydroxy-Trans-Stilbene Induces Apoptosis and Autophagy in Human Non-Small-Cell Lung Cancer Cells In Vitro Treatment with 3,4,4′-trihydroxy-trans-stilbene concentration-dependently inhibited cell viability. It did not cause cell necrosis, but induced apoptosis accompanied by up-regulation of cleavaged PARP, caspase3/9 and Bax, and by down-regulation of Bcl-2 and surviving. [Acta Pharmacol Sin] Abstract 7-Epiclusianone, a Benzophenone Extracted from Garcinia brasiliensis (Clusiaceae), Induces Cell Cycle Arrest in G1/S Transition in A549 Cells The authors investigated the mechanisms involved with antiproliferative activity of 7-epiclusianone in A549 cells. They showed that 7-epiclusianone reduced the viability of A549 cells in a concentration-dependent manner. [Molecules] Abstract | Download Full Article |