In Vitro Systems Toxicology Assessment of a Candidate Modified Risk Tobacco Product Shows Reduced Toxicity Compared to a Conventional Cigarette Scientists investigated the biological impact of a candidate modified risk tobacco product, the tobacco-heating system 2.2, compared to the 3R4F reference cigarette in normal primary human bronchial epithelial cells. [Chem Res Toxicol] Abstract Cold Stress Increases Reactive Oxygen Species Formation via TRPA1 Activation in A549 Cells Researchers measured the changes of reactive oxygen species (ROS) and the cytosolic Ca2+ concentration ([Ca2+]c) in A549 cells. They observed that cold stress exposure of A549 cell resulted in an increase of ROS and [Ca2+]c, which was completely attenuated by removing Ca2+ from medium. [Cell Stress Chaperones] Abstract Cyclic Mechanical Stretch Down-Regulates Cathelicidin Antimicrobial Peptide Expression and Activates a Pro-Inflammatory Response in Human Bronchial Epithelial Cells Scientists showed that treatment of VA10 cells with vitamin D3 and/or 4-phenyl butyric acid counteracted cyclic stretch mediated down-regulation of cathelicidin antimicrobial peptide mRNA and protein expression. [PeerJ] Full Article LUNG CANCER A Novel Naphthalimide Compound Restores p53 Function in Non-Small-Cell Lung Cancer by Reorganizing the Bak-Bcl-xl Complex and Triggering Transcriptional Regulation NA-17 showed p53-dependent inhibition selectivity in different non-small-cell lung cancer cell lines due to the activation state of endogenous p53 in the background level. Further studies revealed that NA-17 caused cell cycle arrest at the G1 phase, changed cell size, induced apoptosis and cell death by increasing the proportion of sub-G1 cells. [J Biol Chem] Abstract | Full Article In Vivo Delivery of miR-34a Sensitizes Lung Tumors to Radiation through RAD51 Regulation Investigators demonstrated that miR-34a directly binds to the 3’ untranslated region of RAD51 and regulates homologous recombination, inhibiting double-strand-break repair in non-small-cell lung cancer cells. [Mol Ther Nucleic Acids] Full Article A Spontaneous Metastasis Model Reveals the Significance of Claudin-9 Overexpression in Lung Cancer Metastasis Scientists developed a spontaneous metastasis model that retains the egression step of metastasis. By repeated in vivo passaging of the poorly metastatic Lewis lung carcinoma cells, they generated a cell line that readily metastasizes to lungs and liver from subcutaneous tumors. [Clin Exp Metastasis] Abstract Γ-Ionizing Radiation-Induced Activation of the EGFR–p38/ERK–STAT3/CREB-1–EMT Pathway Promotes the Migration/Invasion of Non-Small Cell Lung Cancer Cells and Is Inhibited by Podophyllotoxin Acetate Researchers report a new intracellular signaling pathway involved in γ-ionizing radiation (IR)-induced migration/invasion and show that podophyllotoxin acetate inhibits the IR-induced invasion and migration of A549 cells. [Tumor Biol] Abstract Rap2b Promotes Proliferation, Migration, and Invasion of Lung Cancer Cells The authors investigated the effect of Rap2b on the lung cancer malignant phenotype, such as cell proliferation and metastasis. They found that Rap2b could promote the abilities of lung cancer cell wound healing, migration, and invasion via increasing matrix metalloproteinase-2 enzyme activity. [J Recept Signal Transduct Res] Abstract |