Surfactant Dysfunction during Over-Expression of TGF-β1 Precedes Profibrotic Lung Remodeling In Vivo Transforming growth factor beta1 (TGF-β1) effects on pulmonary structure and function were analyzed. Adenoviral mediated gene-transfer of TGF-β1 in mice lungs was performed and evaluated by design-based stereology, invasive pulmonary function testing and detailed analyses of the surfactant system one and two weeks after gene-transfer. [Am J Physiol Lung Cell Mol Physiol] Abstract The Vitronectin RGD Motif Regulates TGFβ Induced Alveolar Epithelial Cell Apoptosis To determine the role of specific matrix proteins on TGFβ-induced apoptosis, the authors studied primary alveolar epithelial cells cultured on different matrix conditions and utilized mice with deletion of vitronectin or mice in which the vitronectin RGD motif is mutated to non-integrin binding RGE. [Am J Physiol Lung Cell Mol Physiol] Abstract Linocin and OmpW Are Involved in Attachment of the Cystic Fibrosis-Associated Pathogen Burkholderia cepacia Complex to Lung Epithelial Cells and Protect Mice against Infection Scientists developed a new proteomics approach to identify bacterial proteins that are involved in the attachment of B. cepacia complex (Bcc) bacteria to lung epithelial cells. Fourteen proteins were reproducibly identified by two-dimensional gel electrophoresis from four Bcc strains representative of two Bcc species: B. cenocepacia, the most virulent, and B. multivorans, the most frequently acquired. [Infect Immun] Abstract miR-125b Inhibits Goblet Cell Differentiation in Allergic Airway Inflammation by Targeting SPDEF Expressions of miR-125b and SAM pointed domain-containing ETS transcription factor (SPDEF) in normal human tracheal epithelial and A549 cells stimulated with lipopolysaccharide for 2 hours were detected by qRT-PCR and western blot. [Eur J Pharmacol] Abstract LUNG CANCER Artemisinin and Its Derivatives Can Significantly Inhibit Lung Tumorigenesis and Tumor Metastasis through Wnt/β-Catenin Signaling Scientists used flow cytometry to show that artemisinin, dihydroartemisinin, and artesunate could inhibit the proliferation of A549 and H1299 cells by arresting cell cycle in G1 phase. Meanwhile, tumor malignancy including migration, invasion, cancer stem cells, and epithelial–mesenchymal transition were also significantly suppressed by these compounds. [Oncotarget] Full Article Oncogenic ALK Regulates EMT in Non-Small Cell Lung Carcinoma through Repression of the Epithelial Splicing Regulatory Protein 1 Investigators found that the anaplastic lymphoma kinase (ALK)-rearranged H2228 and DFCI032, but not the H3122, cell lines displayed a mesenchymal phenotype. In these cell lines, oncogenic ALK activity dictated an epithelial-mesenchymal transition (EMT) phenotype by directly suppressing E-cadherin and up-regulating vimentin expression, as well as expression of other genes involved in EMT. [Oncotarget] Abstract Reciprocal Negative Regulation between EGFR and DEPTOR Plays an Important Role in the Progression of Lung Adenocarcinoma A series of assays conducted in DEP domain-containing mTOR-interacting protein (DEPTOR) knockout or ectopic expression in A549 cells confirmed that DEPTOR inhibited proliferation, migration, and invasion as well as the in vivo tumor growth of lung adenocarcinoma. [Mol Cancer Res] Abstract Combined Inhibition of EZH2 and HDACs as a Potential Epigenetic Therapy for Non-Small Cell Lung Cancer Cells Researchers determined the effects of co-treatment with 3-deazaneplanocin A and the histone deacetylase inhibitor vorinostat in non-small cell lung cancer (NSCLC) cells. The co-treatment synergistically suppressed the proliferation of all tested NSCLC cell lines, regardless of their EGFR status. [Cancer Sci] Abstract |