Pressure Increases PD-L1 Expression in A549 Lung Adenocarcinoma Cells and Causes Resistance to Anti-ROR1 CAR T Cell-Mediated Cytotoxicity

Researchers constructed αROR1-CAR T cells and co-cultured with A549 cells with and without elevated pressure. They then measured apoptosis and cell death by flow cytometry and luciferase activity. They also measured cytokine release by ELISA.